Featured
-
-
Article
| Open AccessSTRA8–RB interaction is required for timely entry of meiosis in mouse female germ cells
Female germ cells initiate meiosis within a limited time period in the fetal ovary. Here the authors show that the interaction between STRA8 and RB ensures precise timing of meiosis initiation and highlight the regulatory mechanisms underlying female-specific meiotic initiation in mice.
- Ryuki Shimada
- , Yuzuru Kato
- & Kei-ichiro Ishiguro
-
Article
| Open AccessPhase separation of BuGZ regulates gut regeneration and aging through interaction with m6A regulators
Phase separation serves to compartmentalize and concentrate cellular components to facilitate essential physiological processes. Here, the authors elucidate the role and mechanism of BuGZ-mediated phase separation in the context of gut regeneration and aging.
- Qiaoqiao Zhang
- , Kai Deng
- & Hao Jiang
-
Article
| Open AccessMetabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction
How cellular metabolism is connected to differentiation remains poorly understood. Here the authors report a regulatory cascade in which circulating citrate regulates sperm production by controlling protein stability via a specific protein post-translational modification.
- Charlotte M. François
- , Thomas Pihl
- & Bruno Hudry
-
Article
| Open AccessA DEAD-box helicase drives the partitioning of a pro-differentiation NAB protein into nuclear foci
The mechanism of spatiotemporal gene regulation during animal development is a fundamental question in biology. Here the authors show that the DEAD-box helicase DDX-23 controls stem cell fate by driving the formation of NAB repressive transcriptional condensates.
- Akiko Doi
- , Gianmarco D. Suarez
- & H. Robert Horvitz
-
Article
| Open AccessA spatial sequencing atlas of age-induced changes in the lung during influenza infection
Ageing is known to impair the immune response against infectious pathogens. Here, Kasmani et al. present a spatial and transcriptomic atlas of immune changes in the lungs of young and aged mice in response to influenza virus infection.
- Moujtaba Y. Kasmani
- , Paytsar Topchyan
- & Weiguo Cui
-
Article
| Open AccessConserved enhancers control notochord expression of vertebrate Brachyury
This study identifies three conserved shadow enhancers (T3, C, I) regulating notochord expression of Brachyury across vertebrates using genomic data, transgenic assays, and enhancer knockouts, which are critical for vertebrate notochord development.
- Cassie L. Kemmler
- , Jana Smolikova
- & Alexa Burger
-
Article
| Open AccessThe admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genes
The brushtail possum is a treasured Australian marsupial, but also a harmful pest introduced into New Zealand. Here, using functional genomics and a new chromosome-level genome assembly of New Zealand possums, Bond et al. quantify their genome admixture and identify unique parent-specific and weaning associated gene expression.
- Donna M. Bond
- , Oscar Ortega-Recalde
- & Timothy A. Hore
-
Article
| Open AccessALKBH5 controls the meiosis-coupled mRNA clearance in oocytes by removing the N 6-methyladenosine methylation
N6-methyladenosine (m6A) maintains maternal RNA stability in oocytes. Here, the authors identify demethylase ALKBH5 as a key determinant of oocyte quality and unveil the facilitating role of ALKBH5-mediated m6A removal in maternal RNA decay.
- Long Bai
- , Yu Xiang
- & Yimin Zhu
-
Article
| Open AccessFGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation
Restoring salivary acinar cells after gland damage is a major clinical challenge. Here, authors identify FGF7-FGFR2-MAPK signaling as a regenerative target, critical for myoepithelial-acinar crosstalk that regulates seromucous acinar differentiation.
- Marit H. Aure
- , Jennifer M. Symonds
- & Matthew P. Hoffman
-
Article
| Open AccessStochastic gene expression and environmental stressors trigger variable somite segmentation phenotypes
Mutations of several genes cause variable phenotypes. Here, the authors show stochastic her1 and her7 gene expression, which is affected by environmental conditions, underlies variability of somite segmentation defects.
- Kemal Keseroglu
- , Oriana Q. H. Zinani
- & Ertuğrul M. Özbudak
-
Article
| Open AccessKDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitors
Zhang et al. report that KDM2B-∆CxxC activated Wnt signaling in the developing hippocampi, where the migration and differentiation of neural progenitors were blocked. KDM2B-∆CxxC mice exhibited defects of hippocampal morphology and related behaviors.
- Bo Zhang
- , Chen Zhao
- & Yan Zhou
-
Article
| Open AccessMulti-species atlas resolves an axolotl limb development and regeneration paradox
Single-cell transcriptomic cross-species comparison identifies critical limb developmental cell type apical-ectodermal ridge in the prime limb regeneration model axolotl, and its differential usage during amphibian limb regeneration.
- Jixing Zhong
- , Rita Aires
- & Can Aztekin
-
Article
| Open AccessUnaltered hepatic wound healing response in male rats with ancestral liver injury
How much the environment influences inherited adaptive traits is debated and challenging to demonstrate in mammals. Here the authors performed a multigeneration study that failed to morphologically replicate enhanced wound healing response following ancestral liver injury in rats. However, heritable transcriptional effects suggest transmission at the molecular level, albeit of unclear functional relevance.
- Johanna Beil
- , Juliane Perner
- & Rémi Terranova
-
Article
| Open AccessLuminal Rank loss decreases cell fitness leading to basal cell bipotency in parous mammary glands
Rocha and co-authors show that loss of luminal Rank signaling causes abnormal alveolar differentiation and lactation failure. Subsequent pregnancies activate bipotency in basal cells, replacing unfit luminal cells, and restoring lactation.
- Ana Sofia Rocha
- , Alejandro Collado-Solé
- & Eva González-Suárez
-
Article
| Open AccessThe endoribonuclease Arlr is required to maintain lipid homeostasis by downregulating lipolytic genes during aging
Fat is generally considered to have a negative effect on longevity but lipids can be beneficial for longevity. Here the authors show that fly Arlr endoribonuclease affects lipid metabolism by degrading the mRNAs of lipolysis genes.
- Xiaowei Sun
- , Jie Shen
- & Dan Wang
-
Article
| Open AccessThe USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6
Wnt receptor levels drive signaling. Here the authors find that precise signaling in the Wnt morphogen gradient relies on Usp46, a deubiquitylase that enhances target cells sensitivity by increasing cell surface levels of the Wnt receptor Arrow/LRP6.
- Zachary T. Spencer
- , Victoria H. Ng
- & Yashi Ahmed
-
Article
| Open AccessMacroscopic waves, biological clocks and morphogenesis driven by light in a giant unicellular green alga
Self-organised waves propagate throughout the alga Caulerpa. Light temporal patterns control the waves and algal morphology, potentially tying light-synchronized self-oscillations to one of the mysteries of single-cell development, morphogenesis.
- Eldad Afik
- , Toni J. B. Liu
- & Elliot M. Meyerowitz
-
Article
| Open AccessIFNγ-Stat1 axis drives aging-associated loss of intestinal tissue homeostasis and regeneration
Omrani, Krepelova et al. report that aging-induced proinflammatory IFNγ/Stat1 signalling primes intestinal stem cells to a secretory fate and to antigen presenting cells impairing the regenerative capacity of the aging gut epithelium.
- Omid Omrani
- , Anna Krepelova
- & Francesco Neri
-
Article
| Open AccessPAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development
A coding variant of the PAX4 transcription factor (p.Arg192His) is uniquely associated with Type 2 Diabetes in East Asian populations. Here, the authors show that two different coding gene variants of PAX4, p.Arg192His and the newly identified p.Tyr186X, can influence pancreatic beta cell development, identity, and function.
- Hwee Hui Lau
- , Nicole A. J. Krentz
- & Adrian Kee Keong Teo
-
Article
| Open AccessC. elegans germ granules sculpt both germline and somatic RNAome
Germ granules are membraneless organelles that act as organizing centers for small RNA biogenesis during germline development. Here they show the LOTUS domain protein eggd-1 organizes germ granules, and reveal a germ line-to-soma communication pathway activated upon perturbation of germ granules.
- Ian F. Price
- , Jillian A. Wagner
- & Wen Tang
-
Article
| Open AccessSMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease
Here the authors reveal that a neomorphic mutation in chromatin protein SMCHD1 enhances SMCHD1-mediated gene silencing, including at the FSHD disease-relevant locus, while depleting SMCHD1-mediated chromatin interactions, suggesting these SMCHD1 functions are unlinked.
- Andres Tapia del Fierro
- , Bianca den Hamer
- & Marnie E. Blewitt
-
Article
| Open AccessMaintenance of pluripotency-like signature in the entire ectoderm leads to neural crest stem cell potential
How the neural crest gains its pluripotency-like stem cell potential is unclear. Here, the authors show that the entire post-gastrula ectoderm maintains expression of pluripotency genes, leading to the high stem cell capacity in the neural crest.
- Ceren Pajanoja
- , Jenny Hsin
- & Laura Kerosuo
-
Article
| Open AccessNon-uniform temporal scaling of developmental processes in the mammalian cortex
Marsupial mammals take much longer to develop than similarly sized placental mammals, though how brain development occurs across these different periods is unclear. Here they show that the neurodevelopmental events of cortical neurogenesis, cell migration and axon extension do not all temporally scale to the same extent.
- Annalisa Paolino
- , Elizabeth H. Haines
- & Laura R. Fenlon
-
Article
| Open AccessA branching model of lineage differentiation underpinning the neurogenic potential of enteric glia
The enteric nervous system encompasses the gut-intrinsic neuroglial networks that regulate gastrointestinal functions. Based on single-cell analysis, the authors propose a model in which neurogenic differentiation paths branch from a gliogenic trajectory.
- Anna Laddach
- , Song Hui Chng
- & Vassilis Pachnis
-
Article
| Open AccessThe TNFR Wengen regulates the FGF pathway by an unconventional mechanism
Mechanistic studies of receptor action have aided our understanding of developmental processes and facilitated drug development. Here they show that the TNFR-Wengen acts by forming a complex with the FGFR-Breathless, regulating its activity during cell differentiation in the developing respiratory system of Drosophila.
- Annalisa Letizia
- , Maria Lluisa Espinàs
- & Marta Llimargas
-
Article
| Open AccessSelf-organized and directed branching results in optimal coverage in developing dermal lymphatic networks
The lymphatic system is a transport network that controls immune response and tissue fluid circulation in the body. Here the authors combine experiment and theory to reveal that developing lymphatic capillary networks exploit complementary branching strategies to optimize tissue coverage.
- Mehmet Can Uçar
- , Edouard Hannezo
- & Kari Vaahtomeri
-
Article
| Open AccessFgf signalling triggers an intrinsic mesodermal timer that determines the duration of limb patterning
The limb bud is patterned by crosstalk between the mesoderm and the overlying apical ectodermal ridge, but it has been difficult to determine the requirement for different ligands in this process. Here the authors use a chick wing explant system to show that fibroblast growth factors trigger a mesodermal programme that is key for timing limb bud patterning.
- Sofia Sedas Perez
- , Caitlin McQueen
- & Matthew Towers
-
Article
| Open AccessInterlocking of co-opted developmental gene networks in Drosophila and the evolution of pre-adaptive novelty
During evolution, genes can be recruited to new positions to perform novel functions. This study shows one such co-option event, where the reused gene networks are initially interlocked, so that any changes because of their function in one organ are mirrored in the other organs even if they provide no selective advantage, opening the potential for acquiring a novel function.
- Sara Molina-Gil
- , Sol Sotillos
- & James C.-G. Hombría
-
Article
| Open AccessMultimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis
Multimodal spatiotemporal transcriptomic resolution of palatal osteogenesis identifies previously unreported enriched genes in developing palate, paving the way toward viable diagnostic and therapeutic targets for cleft palate disorders.
- Jeremie Oliver Piña
- , Resmi Raju
- & Rena N. D’Souza
-
Article
| Open AccessActivator-blocker model of transcriptional regulation by pioneer-like factors
How gene expression timing is regulated during development remains a key area of research. Here they show that zebrafish genome activators Pou5f3 and Nanog block each other’s activity on the enhancers of differentiation genes, preventing their premature expression.
- Aileen Julia Riesle
- , Meijiang Gao
- & Daria Onichtchouk
-
Article
| Open AccessMonolayer platform to generate and purify primordial germ-like cells in vitro provides insights into human germline specification
Generation of primordial germ cell-like cells (PGCLCs) from human pluripotent cells (hPSCs) offers insight into the mechanisms underlying human reproduction, but often requires complex methods. Here they describe a simplified monolayer protocol to differentiate and purify PGCLCs for further analysis.
- Sivakamasundari Vijayakumar
- , Roberta Sala
- & Vittorio Sebastiano
-
Article
| Open AccessTime space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
Gastrulation entails a series of events that are highly coordinated in space and time. Here they construct a spatiotemporal molecular atlas of lineage trajectories in the gastrulating mouse embryo by mapping single cells to spatial coordinates in the germ layers with reference to positional data in the transcriptome.
- Ran Wang
- , Xianfa Yang
- & Naihe Jing
-
Article
| Open AccessGut barrier defects, intestinal immune hyperactivation and enhanced lipid catabolism drive lethality in NGLY1-deficient Drosophila
NGLY1 mutations cause a multisystem developmental disorder. Here they show that this enzyme is required for normal gut barrier function, and when mutated, causes immune and metabolic abnormalities, contributing to lethality.
- Ashutosh Pandey
- , Antonio Galeone
- & Hamed Jafar-Nejad
-
Article
| Open AccessCell diversity and plasticity during atrioventricular heart valve EMTs
Lotto et al. delineate cell diversity and mechanisms during heart valve development using scRNA-seq. They identify distinct cell types and states, the emergence of epithelial-mesenchymal plasticity, and cell interactions that may govern this process.
- Jeremy Lotto
- , Rebecca Cullum
- & Pamela A. Hoodless
-
Article
| Open AccessSerotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages
Serotonin signaling is well known for modulating animal behavior. Here the authors discovered it plays a developmental role in regulating actomyosin contractility driven cellular mechanics and tissue flows during gastrulation of insect and bird.
- Sanjay Karki
- , Mehdi Saadaoui
- & Thomas Lecuit
-
Article
| Open AccessCoordination of alternative splicing and alternative polyadenylation revealed by targeted long read sequencing
In this study using a targeted long read RNA sequencing approach called PL-Seq, the authors uncover coordination of alternative splicing and alternative polyadenylation within individual genes in Drosophila melanogaster.
- Zhiping Zhang
- , Bongmin Bae
- & Pedro Miura
-
Article
| Open AccessSinus venosus adaptation models prolonged cardiovascular disease and reveals insights into evolutionary transitions of the vertebrate heart
Nr2fs are conserved transcription factors that regulate atrial chamber and venous development. Here, the authors use adult zebrafish nr2f1a mutants to investigate compensatory remodeling of the inflow tract and hypotheses of cardiac evolution.
- Jacob T. Gafranek
- , Enrico D’Aniello
- & Joshua S. Waxman
-
Article
| Open AccessNotch and retinoic acid signals regulate macrophage formation from endocardium downstream of Nkx2-5
A subset of endocardial cells gives rise to hematopoietic cells which are important for the formation of cardiac valves. In this follow up study, authors report the molecular regulatory mechanisms behind the formation of macrophages in the heart.
- Norika Liu
- , Naofumi Kawahira
- & Atsushi Nakano
-
Article
| Open AccessPlacental growth factor exerts a dual function for cardiomyogenesis and vasculogenesis during heart development
Growth factors play key roles during heart development. Here they show that PLGF has both autocrine and paracrine roles during cardiomyogenesis and vasculogenesis, suggesting it may have therapeutic potential for heart disease.
- Nevin Witman
- , Chikai Zhou
- & Makoto Sahara
-
Article
| Open AccessDeciphering early human pancreas development at the single-cell level
Here, the authors revealed molecular heterogeneity, developmental trajectory and regulatory network of early human pancreas development, and depict the whole progression of pancreatic organogenesis during the first trimester at the single-cell level.
- Zhuo Ma
- , Xiaofei Zhang
- & Tao Xu
-
Article
| Open AccessEpigenetic inheritance is unfaithful at intermediately methylated CpG sites
Here the authors question the prevalent view of DNA methylation as a stably inherited epigenetic mark, revealing that the existing model does not apply when considering genomic loci that are intermediately methylated.
- Amir D. Hay
- , Noah J. Kessler
- & Anne C. Ferguson-Smith
-
Article
| Open AccessNacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila
Protein homeostasis is crucial for maintaining cellular homeostasis. Here, the authors show that proteotoxic stress caused by Nacalpha mutants specifically and progressively induces cell death in the apoptosis-resistant Drosophila larval fat body.
- Takayuki Yamada
- , Yuto Yoshinari
- & Takashi Nishimura
-
Article
| Open AccessTargeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development
Highly localized mechanical forces that shape in vivo tissue development remain challenging to recapitulate in vitro. Here the authors use magnetically actuated nanoparticles to generate spatially defined forces within organoids, which guide the spatial organization of tissue patterning and growth.
- Abdel Rahman Abdel Fattah
- , Niko Kolaitis
- & Adrian Ranga
-
Article
| Open AccessThe non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters
The role of PIWI proteins in mammalian reproduction remains poorly understood. Here, using golden hamsters, the authors establish expression profiles for all four PIWIs during gametogenesis and characterize their associated reproductive defects.
- Xiaolong Lv
- , Wen Xiao
- & Ligang Wu
-
Article
| Open AccessA transcriptional response to replication stress selectively expands a subset of Brca2-mutant mammary epithelial cells
Here the authors study how BRCA2 mutations affect mammary epithelial subpopulations. They report that Brca2mut/WT mammary organoids subjected to replication stress activate a transcriptional response that selectively expands Brca2mut/WT HR- luminal cells.
- Maryam Ghaderi Najafabadi
- , G. Kenneth Gray
- & Mona Shehata
-
Article
| Open AccessSYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility
The cytoplasmic droplet is an elusive structure of mammalian sperm. Here, the authors uncover a key gene that defines a vesicular pathway essential for cytoplasmic droplet formation and male fertility.
- Jiali Liu
- , Louis Hermo
- & Chen Chen
-
Article
| Open AccessBat teeth illuminate the diversification of mammalian tooth classes
Despite their key role in the evolution of mammals, the origin and diversification of tooth classes (incisors, canines, premolars and molars) remain relatively obscure. Here the authors use integrative approaches to explain the independent evolution and rapid diversification of two tooth classes, premolar and molars, in noctilionoid bats.
- Alexa Sadier
- , Neal Anthwal
- & Karen E. Sears
-
Article
| Open AccessEarly-adulthood spike in protein translation drives aging via juvenile hormone/germline signaling
The transient elevation in protein translation during early-adulthood in Drosophila imposes long-lasting negative impacts on future aging trajectories by triggering proteostatic dysfunction at old ages.
- Harper S. Kim
- , Danitra J. Parker
- & Andrew M. Pickering
-
Article
| Open AccessATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expression
Muscle regeneration relies on activation and expansion of skeletal muscle stem cells. Here, authors show that ATF3 induction prevents precocious activation of skeletal muscle stem cells by binding and promoting the transcription of Histone2B.
- Suyang Zhang
- , Feng Yang
- & Huating Wang
Browse broader subjects
Browse narrower subjects
- Ageing
- Angiogenesis
- Bone development
- Bone remodelling
- Cartilage development
- Cell growth
- Cell proliferation
- Ciliogenesis
- Differentiation
- Disease model
- Embryogenesis
- Embryology
- Epigenetic memory
- Experimental organisms
- Germline development
- Haematopoiesis
- Intrauterine growth
- Lymphangiogenesis
- Morphogenesis
- Neurogenesis
- Organogenesis
- Pattern formation
- Pluripotency
- Reprogramming
- Self-renewal
- Senescence
- Stem-cell niche
- Stem cells
- Transdifferentiation