News & Comment

Blanpain and colleagues provide evidence that the small RHO GTPase, RHOJ, mediates resistance to chemotherapy in tumour cells that have undergone epithelial-to-mesenchymal transition by enabling these cells to tolerate replicative stress and promote DNA damage repair.

In this Tool of the Trade article, Tatiana Flisikowska describes the development and use of humanized minipigs that allow the preclinical safety testing of human therapeutic antibodies.

In this Tool of the Trade article, Mateusz Legut describes the development and use of OverCITE-seq, a new single-cell multiomics approach enabling the high-throughput quantification of the transcriptome and surface antigens in cells expressing a library of open reading frames.

In this study, Weems et al. demonstrate that detachment-induced dynamic blebbing leads to the assembly of pro-survival signalling molecules at the plasma membrane, which protects melanoma cells from anoikis.

Han et al. employed an in vivo imaging workflow that coupled positron emission tomography imaging to micro-computed tomography and 3D serial block-face electron microscopy to produce a detailed structural and functional map of mitochondrial networks in lung cancer.

In this Tools of the Trade article, Alexandros P. Drainas describes the development and use of EpicMIBI, a multiplexed imaging technology that enables the study of spatial clonality in cancer.

In this study, Bhardwaj et al. highlight the mechanistic link between elevated body mass index and breast epithelial cell DNA damage in individuals carrying BRCA mutations.

In this article, Efrat Shema describes EPINUC, a liquid biopsy method based on epigenetic profiling of nucleosomes from cell-free DNA in the plasma. EPINUC, combined with protein biomarker measurements, enables the accurate differentiation of samples from healthy volunteers and patients.

In this Tools of the Trade article, Francisco Barriga describes the development and use of MACHETE, a flexible deletion engineering strategy, which enables the functional characterization of the large genomic deletions that occur in cancer.

In this study, the authors investigate the mechanistic basis of alternative telomere lengthening, a process that certain cancer cells use to overcome telomere shortening.

Altea-Manzano et al. find that factors secreted by primary breast tumours or high-fat diet feeding enriches the fatty acid palmitate in distant organs, which primes pre-metastatic niches enabling metastatic growth.

In this study, Linde et al. demonstrate how therapeutic activation of neutrophils leads to tumour eradication and reduced metastatic seeding in multiple mouse models of cancer.

In this Tools of the Trade article, Susan Bullman describes the development and use of INVADEseq, a single-cell RNA sequencing approach that facilitates the simultaneous capture of both eukaryotic and bacterial transcripts in host cells to functionally understand the intratumoural microbiota.

In this Tools of the Trade article, Lee describes Select-seq, a novel method for generating epitranscriptomic data from microniches within the tumour. The author reveals how the method can be used to identify novel biomarkers from rare cell subpopulations such as cancer stem cells in tumours.

In this study, Jung-Garcia et al. use in vitro and in vivo modelling to demonstrate the role of LAP1 in promoting melanoma cell invasion and highlight its link to metastatic dissemination.

This study shows how the selective immune pressure in early-stage tumours drives interferon-γ-dependent metabolic reprogramming in cancer cells to mediate immune escape.

To understand malignant progression, Yuan et al. delineate the complex crosstalk between cancer stem cells and their microenvironment that is initiated by oncogenic RAS.

In a study in Nature, Wang et al. describe how circadian rhythms can impact tumour suppression through their effects on dendritic cells, a finding that could help to optimize clinical trials of cancer immunotherapies.