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The authors present a simple and cost-efficient approach for the covalent coupling of enzymes on solid supports or for the intermolecular cross-linking of enzymes.
This protocol describes cfSNV, a user-friendly software package that comprehensively considers the unique properties of cell-free DNA for the sensitive detection of somatic mutations from blood samples.
In many environmental or clinical settings, it is useful to detect and quantify analytes instantaneously in situ. This protocol describes how to develop metal–organic frameworks for selective, sensitive analysis by luminescence.
A robust, mild and fast approach for the posttranslational, site-directed fluorination of protein sidechains, detectable via 19F-based magnetic resonance methods.
The authors present a protocol for the generation of human blastoids—structures morphologically and transcriptionally resembling complete blastocysts—from pluripotent stem cells and for their use in modeling implantation into hormonally stimulated endometrial cells.
This protocol for concomitant high-throughput mitochondrial DNA genotyping and accessible chromatin profiling of single cells allows paired analysis of clonal relationships and cell states.
The authors present a protocol for the modular 3D bioengineering of multilineage skeletal muscles from human induced pluripotent stem cells, along with assays to characterize morphological and functional features of the artificial muscle constructs.
The tandem mass spectrometers used in clinical chemistry are expensive. This protocol describes how to generate similar results using a single mass spectrometry detector by optimizing in-source fragmentation and data analysis via correlated ion monitoring.
The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.
This protocol describes a solvothermal-based process to prepare gram-scale ferrite nanoparticles with well-defined shapes (nanocubes, nanostars, faceted and spherical) having heating properties appealing for clinical magnetic hyperthermia treatments.
Single-cell screening and sorting is useful for addressing many biological questions. The high-throughput droplet analyzer and sorter described in this protocol has been designed so that it can be constructed without specialist engineering training.
A full-body nanobody-based immunolabeling and clearing method that renders mice transparent in 3 weeks, enhancing the signal of fluorescent proteins and allowing their reliable detection and quantification, at cellular resolution, within an entire body.
We present a protocol for the synthesis and usage of QM-FN-SO3, a near-infrared aggregation-induced-emission-active fluorescent probe capable of crossing the blood–brain barrier and ultrasensitively lighting up amyloid-β plaques in living mice.
The amplification of misfolded alpha-synuclein aggregates in vitro can be used for the detection, via fluorescent dyes, of pathologic amyloids in cerebrospinal fluid samples from patients affected by Parkinson’s disease, dementia with Lewy bodies or multiple-system atrophy.
This protocol describes how to use NetColoc, a freely available tool for evaluating the extent to which two related diseases, each characterized by a set of mapped genes, are colocalized in a reference gene interaction network.
A protocol for quantitative genome-wide detection of replication initiation, fork progression and termination, based on purification and strand-specific sequencing of Okazaki fragments isolated from mammalian or yeast cells.
Making microfluidic chips using 3D printers allows rapid fabrication of ready-to-use products from digital 3D designs with minimal human intervention. This ‘print–pause–print’ protocol describes how to make transparent, multimaterial chips.
This protocol combines shell-isolated nanoparticle-enhanced Raman spectroscopy and ab initio molecular dynamics simulations to unravel the directional molecular features of interfacial water, enabling a better understanding of electrocatalysis.
A surgical procedure that preserves hearing while creating an imaging window in the mouse cochlea, enabling the functional imaging of cochlear cells in vivo.
This protocol describes an in vitro vesicle formation system to reconstitute, purify and characterize plant-derived coat protein complex II vesicles from Arabidopsis thaliana suspension-cultured cells.