Volume 15 Issue 10, October 2018

Speeding up spot-to-plunge in cryo-EM and how to keep a lab talking.

By incorporating an image-classification task into an online video game, the Human Protein Atlas project unlocks the gaming community as a citizen science force.

By coupling multiplex iterative indirect immunofluorescence imaging with computer vision methods, researchers can detect at least 40 different proteins with subcellular resolution.

CRISPR–Cas9 enables successive fusions of 16 native chromosomes into one artificial single chromosome.

Studies of tumor immunology will gain from coculture of tumor organoids and peripheral blood cells.

FluoEM aligns axon reconstructions from fluorescence and 3D electron microscopy on the same tissue to allow multicolor labeling of distant inputs.

A way to gene edit without double-stranded DNA breaks is now entering labs.

Building on methods from electron microscopy, researchers are applying nanoscale fluorescence imaging to address questions in structural biology.

A new article by Pandarinath et al. describes an artificial neural network model that captures some key aspects of the activity of populations of neurons in the primary motor cortex.

A new detector built for X-ray free-electron lasers provides unprecedented speed and accuracy for macromolecular crystallography at synchrotron radiation facilities—and finally allows crystallographers to harness the full capabilities of those sources.

A computational and analytical framework enables multicolor 3D particle reconstruction of protein complexes from 2D images. The authors demonstrate the power of the approach by reconstructing native proteins within the human centriole.

An all-to-all registration approach allows for improved, high-resolution, template-free single-particle reconstruction from localization microscopy data under realistic experimental conditions such as low labeling density.

In vivo probing of RNA structures with COMRADES yields insight into RNA folding of the ZIKA virus genome and its interaction with host RNAs.

Wang et al. demonstrate that the effects of aberrations and scattering caused by the mouse skull can be reduced with three-photon microscopy. Their approach allows structural and functional imaging of the brain through an intact skull.

Reducing the length of time that protein particles spend on a sample grid prior to freezing mitigates deleterious effects caused by particle adsorption to the air–water interface in single-particle cryo-EM.

The Qiita web platform provides access to large amounts of public microbial multi-omic data and enables easy analysis and meta-analysis of standardized private and public data.

A charge-integrating pixel-array detector called JUNGFRAU enables the collection of highly accurate X-ray crystallography data at synchrotron sources at unprecedented speeds.

LFADS, a deep learning method for analyzing neural population activity, can extract neural dynamics from single-trial recordings, stitch separate datasets into a single model, and infer perturbations, for example, from behavioral choices to these dynamics.

DeepSequence is an unsupervised deep latent-variable model that predicts the effects of mutations on the basis of evolutionary sequence information.

The synOptopatch approach enables all-optical access to synaptic communication via mutually exclusive expression of an optogenetic actuator and a voltage sensor in pre- and postsynaptic neurons, respectively.

TECtool identifies terminal exons from RNA-seq data, uncovering novel isoforms, many of which are translated.