Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
Measuring force exerted on an oil microdroplet within living embryonic mouse tissue. Cover by Erin Dewalt, based on an image provided by Otger Campàs. Article p183
Bar charts are too frequently used to communicate data that they cannot represent well. We strongly encourage the use of more appropriate plots to display statistical samples.
A new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics while minimizing artifacts caused by movement.
Optofluidic biolasers are emerging as a highly sensitive way to measure changes in biological molecules. Biolasers, which incorporate biological material into the gain medium and contain an optical cavity in a fluidic environment, take advantage of the amplification that occurs during laser generation to quantify tiny changes in biological processes in the gain medium. This Perspective describes the principle of the optofluidic biolaser, reviews recent progress and provides outlooks on potential applications and directions for developing this enabling technology.
This Analysis reports the development and assessment of 645 multiple reaction monitoring (MRM) mass spectrometry assays to quantify 319 targeted human breast cancer proteins. The results of this pilot project coordinated among three individual groups suggest that an organized international effort to generate MRM assays to the human proteome will be possible.
A system using the human glycine receptor expressed in Xenopus oocytes allows characterization of the photoactivation efficiency of photoactivatable and photoconvertible fluorescent proteins at the single-molecule level, providing crucial data for using these probes for quantitative super-resolution microscopy.
With an optimized protocol and unique molecular identifiers (UMIs) to tag individual transcripts, the mRNA complement of a single cell can be quantified on an absolute scale with almost no amplification bias.
A data-independent acquisition (DIA) mass spectrometry approach, ultradefinition (UD)MSE, offers high reproducibility and improved proteome coverage over alternative DIA and data-dependent acquisition workflows.
EC-BLAST is a Web-based tool allowing quantitative similarity searches between enzymes at the levels of bond-change, reaction-center or reaction-structure similarity. The tool may help improve the annotation of enzyme function.
A collection of improved FRET-based calcium biosensors, called Twitch sensors, is described. Twitches have a reduced number of calcium binding sites per sensor and display high sensitivity in in vivo imaging experiments in mouse brain and lymph node.