Ten years of development in light-sheet microscopy have led to spectacular demonstrations of its capabilities. The technology is ready to assist biologists in tackling scientific problems, but are biologists ready for it? Here we discuss the interdisciplinary challenges light-sheet microscopy presents for biologists and highlight available resources.
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References
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).
Huisken, J. & Stainier, D.Y.R. Opt. Lett. 32, 2608–2610 (2007).
Dodt, H.-U. et al. Nat. Methods 4, 331–336 (2007).
Wu, Y. et al. Nat. Biotechnol. 31, 1032–1038 (2013).
Chen, B.-C. et al. Science 346, 1257998 (2014).
Tomer, R., Khairy, K., Amat, F. & Keller, P.J. Nat. Methods 9, 755–763 (2012).
Krzic, U., Gunther, S., Saunders, T.E., Streichan, S.J. & Hufnagel, L. Nat. Methods 9, 730–733 (2012).
Schmid, B. et al. Nat. Commun. 4, 2207 (2013).
Pitrone, P.G. et al. Nat. Methods 10, 598–599 (2013).
Gualda, E.J. et al. Nat. Methods 10, 599–600 (2013).
Kaufmann, A., Mickoleit, M., Weber, M. & Huisken, J. Development 139, 3242–3247 (2012).
Desmaison, A., Lorenzo, C., Rouquette, J., Ducommun, B. & Lobjois, V. J. Microsc. 251, 128–132 (2013).
Wu, J., Li, J. & Chan, R.K.Y. Opt. Express 21, 14474–14480 (2013).
Capoulade, J., Reynaud, E.G. & Wachsmuth, M. in Imaging Marine Life (ed. Reynaud, E.G.) 186–209 (Wiley-VCH, 2013).
Preibisch, S., Saalfeld, S., Schindelin, J. & Tomancak, P. Nat. Methods 7, 418–419 (2010).
Preibisch, S. et al. Nat. Methods 7, 418–419 (2010).
Saalfeld, S., Cardona, A., Hartenstein, V. & Tomancak, P. Bioinformatics 25, 1984–1986 (2009).
Amat, F. et al. Nat. Methods 11, 951–958 (2014).
Eliceiri, K.W. et al. Nat. Methods 9, 697–710 (2012).
Acknowledgements
We thank all members of the ever growing light-sheet community who push this field forward. All participants, speakers and instructors of the first EMBO practical course on light-sheet microscopy in Dresden inspired us to get this piece written. We thank J. Oegema for Figure 3b and L. Gutierrez-Heredia for Figure 2b.
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Reynaud, E., Peychl, J., Huisken, J. et al. Guide to light-sheet microscopy for adventurous biologists. Nat Methods 12, 30–34 (2015). https://doi.org/10.1038/nmeth.3222
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DOI: https://doi.org/10.1038/nmeth.3222
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