Individual cells of the same phenotype are commonly viewed as identical functional units of a tissue or organ. However, the deep sequencing of DNA and RNA from single cells suggests a more complex ecology of heterogeneous cell states that together produce emergent system-level function. Continuing development of high-content, real-time, multimodal single-cell measurement technologies will lead to the ultimate goal of understanding the function of an individual cell in the context of its microenvironment.
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References
Navin, N. et al. Nature 472, 90–94 (2011).
Xu, X. et al. Cell 148, 886–895 (2012).
Buss, L.W. The Evolution of Individuality (Princeton Univ. Press, 1987).
Islam, S. et al. Genome Res. 21, 1160–1167 (2011).
Buckley, P.T. et al. Neuron 69, 877–884 (2011).
Hashimshony, T. et al. Cell Rep. 2, 666–673 (2012).
Shiroguchi, K. et al. Proc. Natl. Acad. Sci. USA 109, 1347–1352 (2012).
Singer, R.H. & Ward, D.C. Proc. Natl. Acad. Sci. USA 79, 7331–7335 (1982).
Sul, J.Y. et al. Proc. Natl. Acad. Sci. USA 106, 7624–7629 (2009).
Kim, T.K. et al. Cell Rep. 5, 114–125 (2013).
Yakubov, E. et al. Biochem. Biophys. Res. Commun. 394, 189–193 (2010).
Sul, J.Y. et al. Trends Biotechnol. 30, 243–249 (2012).
Zangi, L. et al. Nat. Biotechnol. 31, 898–907 (2013).
Kim, J. & Eberwine, J. Trends Cell Biol. 20, 311–318 (2010).
Giannoukos, G. et al. Genome Biol. 13, R23 (2012).
Turnbaugh, P.J. et al. Proc. Natl. Acad. Sci. USA 107, 7503–7508 (2010).
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The aRNA procedure has been patented by the US PTO (#5,514,545, 5,723,290, 5,958,688 and 6,291,170) and the TIPeR procedure has a patent application pending in Pennsylvania. J.-Y.S. and J.K. are also co-inventors on the pending TIPeR patent.
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Eberwine, J., Sul, JY., Bartfai, T. et al. The promise of single-cell sequencing. Nat Methods 11, 25–27 (2014). https://doi.org/10.1038/nmeth.2769
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DOI: https://doi.org/10.1038/nmeth.2769
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