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LiftPose3D infers three-dimensional poses from two-dimensional data or from limited three-dimensional data. The approach is illustrated for videos of behaving Drosophila, mice, rats and macaques.
Long-range CRISPR activation can be enhanced by concurrent recruitment of artificial TFs to the enhancer and promoter of a target gene. This CRISPR activation system can be employed to achieve allele-selective gene upregulation by differentially targeting single-nucleotide polymorphisms embedded in enhancers or other distally located sequences.
Acoustic reporter genes can be imaged with high sensitivity using the BURST imaging sequence, allowing the detection of single cells under optimal conditions.
e2gmm uses a deep neural network with a Gaussian representation to resolve the compositional and conformational variability within biomolecules using cryo-EM data.
RAPID (rapid autofocusing via pupil-split image phase detection) is a sample-agnostic real-time autofocus method for widefield microscopy. RAPID removes most image degradation in large, cleared samples for enhanced quantitative analyses.
CIM-seq offers an unsupervised deconvolution method to profile cell–cell interactions by sequencing cell multiplets of a given tissue, and was employed to analyze diverse tissues such as the intestinal epithelium, lung and spleen.
Paint server and homology optimization pipeline (PaintSHOP), an interactive platform for the design of oligo fluorescence in situ hybridization (FISH) experiments, democratizes and standardizes the process of designing complex probe sets for the oligo FISH community.
SpaceM combines light microscopy and MALDI-imaging mass spectrometry to enable single-cell metabolic profiling of cultured cells. SpaceM reveals coexisting metabolic states of hepatocytes and aims to democratize single-cell metabolomics.
Meta-analysis of more than 200 co-fractionation mass spectrometry experiments provides expanded protein–protein interaction resources and helps establish optimal protocols for experimental design and data analysis.
Multiangle projection imaging accelerates volumetric imaging by up to two orders of magnitude and is readily implemented on diverse microscopes, including spinning disk confocal and light-sheet microscopes.
Single-lysosome mass spectrometry (SLMS) integrates lysosomal patch-clamp recording and induced nanoESI/MS for concurrent metabolic and electrophysiological profiling of individual enlarged lysosomes.
ABEL-FRET combines anti-Brownian trapping for tether-free observation of biomolecules in solution with smFRET. ABEL-FRET offers ultrahigh resolution of FRET efficiency and enables simultaneous hydrodynamic profiling of molecular composition.
Super-resolution structured illumination microscopy reconstruction algorithms are described that can handle structured noise artifacts in two and three dimensions. The algorithms lack adjustable parameters and enhance objective representation of imaged objects.
Detect-seq, built upon chemical labeling and enrichment of intermediate deoxyuridine, offers an approach to profile different types of off-target mutation induced by cytosine base editors including unexpected edits outside of protospacer and on the target strand.
This work presents NeoLoopFinder, a computational method, for identifying chromatin interactions of structurally rearranged genomes. NeoLoopFinder was applied in 50 cancer datasets and identified genes associated with enhancer-hijacking events.