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The combination of a direct electron-detection camera that can count individual electrons and an algorithm for correcting for beam-induced motion in cryo-EM will facilitate determination of three-dimensional structures of smaller, lower-symmetry macromolecular complexes to higher resolution than previously possible.
Unlike hybrid approaches that use multiple libraries for de novo assembly, the hierarchical genome-assembly process uses data from only a single long-read SMRT sequencing library to produce high-quality finished microbial genome or BAC assemblies in an automated workflow.
A method for computing the intrinsic resolution of a super-resolution image that accounts for localization uncertainty, labeling density and image anisotropy is described. This work extends and builds on the Fourier ring correlation method used in cryoelectron microscopy.
A method to measure reversals in gene expression between cell types is used to identify transcriptional regulators important for lineage specification. The approach should help identify putative factors for direct fate conversion.
With a multiplexed, sensitive, selected reaction monitoring–based mass spectrometry approach, transcription factor copy numbers can be accurately quantified during terminal fat cell differentiation.
Differences in substrate adhesion strength are exploited in a label-free approach to separate human pluripotent stem cells from other cell types. This approach can separate fully and partially reprogrammed human induced pluripotent stem cells.
Pairwise siRNA combinations and multiparametric imaging reveal positive and negative genetic interactions of epigenetic regulators in human cancer cells.
Light-sheet microscopy using a laser beam reflected off a mirrored AFM cantilever provides high signal-to-background images suitable for high-speed quantitative single-molecule imaging of transcription factor binding to DNA in the nucleus of living mammalian cells.
Whole-brain imaging of neuronal activity with cellular resolution at almost a brain per second is demonstrated using high-speed light-sheet microscopy in the larval zebrafish brain.
By using a bicistronic design, with a leader peptide that overlaps with and contains the Shine-Dalgarno site for a downstream gene of interest, the authors demonstrate reliable, context-independent gene expression.
This linear ANOVA-based method quantifies the activity of different combinations of genetic elements and assigns a score that indicates the variation in performance across changing contexts.
This paper reports an analytical method for single-particle tracking data. It identifies diffusive states of intracellular proteins and the rates of transition between them.
A combination of clickable, photoreactive sterol probes and mass spectrometry yields a chemoproteomic strategy for profiling protein-cholesterol interaction in living cells.
A single-wavelength genetically encoded sensor of extracellular glutamate is reported. The sensor—iGluSnFR—is bright and photostable under both one- and two-photon illumination and is shown to work for in vivo imaging in worms, zebrafish and mice.
A combination of in situ hybridization and a proximity ligation assay allows the visualization of histone modifications at single genomic loci with single-cell resolution in fixed tissue.
Replication-deficient lentiviral vectors are used as insertional mutagens for cancer gene discovery in the mouse. They are applied to identify oncogenes in hepatocellular carcinoma.
Classifying G protein–coupled receptors by ligand binding similarity leads to unexpected links between receptors unrelated by sequence or structure, possibly revealing insights into receptor evolution.