Articles in 2010

Protein dynamics can be studied in single living cells by time-resolved fluorescent imaging of the unfolding of a fluorescence resonance energy transfer (FRET) probe—labeled protein as fast temperature jumps are applied.

Tumor-initiating cells can be isolated from human glioma or from glioma cultures based on cellular autofluorescence and morphological features alone.

Waltz is a position-specific amyloid-propensity prediction tool developed to distinguish between true amyloid sequences and amorphous β-aggregates.

The fates of cultured neural progenitor cells can be predicted by algorithmic information theory-based computational analysis of time-lapse images of the cells.

A Cre-loxP–based technique allows triggering of heritable coexpression of a fluorescent marker along with any desired transgene, providing a versatile tool for clonal analysis of gene function in the zebrafish.

The use of membrane-tethered toxins to selectively block ion channel function in vivo is demonstrated. The approach is applied to blockade of voltage-gated calcium channels for inhibition of neurotransmission in the mouse.

Microwestern arrays combine the advantages of scalability of reverse phase protein arrays and the information content of western blotting for analyzing protein abundance and modification state with high sensitivity and throughput. The method is demonstrated for analyzing phosphorylation state changes in the EGF receptor signaling network using Bayesian network modeling.