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S-palmitoylation is a protein post-translational modification involved in trafficking, compartmentalization and membrane-tethering of various proteins. A palmitic acid analog, 17-octadecynoic acid, serves as a metabolically incorporated bioorthogonal click chemistry probe for detecting S-palmitoylated human proteins by mass spectrometry or in-gel fluorescence.
Pseudorabies viruses encoding fluorescent proteins are a powerful method for mapping neuronal circuits. Now a series of pseudorabies virus strains encoding fluorescent sensors and time-shifted florescent proteins allow dissection of complex circuits with concurrent activity analysis while defining an analysis period during which the neurons are still healthy.
