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Two incoherently superimposed orthogonal standing waves are used to create a pattern of 116,000 'doughnuts' for fast, highly parallelized coordinate-targeted super-resolution microscopy of living cells, with a large field of view.
RNA polymerase III–driven single guide RNA and a germ line promoter–driven expression of Cas9 enzyme allow heritable, targeted genome modifications in Caenorhabditis elegans.
Designed β-strand peptides stabilize integral membrane proteins for biochemical and structural studies, enabling electron microscopy analysis of the dynamic conformations of the ABC transporter MsbA.
Lipid Blast is an in silico–generated tandem mass spectral library covering more than 119,000 lipid compounds from 26 different classes, providing a useful tool for lipid identification in metabolomics studies.
A multiplexing strategy for data-independent acquisition (DIA)-based mass spectrometry addresses the limitation of low precursor selectivity to make DIA more practical for peptide analysis.
Four spectrally distinct near-infrared fluorescent proteins based on bacterial phytochromes are described, expanding the possibilities for multicolor in vivo imaging experiments in nontransparent organisms.
Conditional genetic knockout is achieved in the rat by using zinc-finger nucleases to place loxP sites at specific genomic locations and introducing Cre recombinase under the control of a native promoter.
A mass spectrometry–based method using serial enrichments of different post-translational modifications (SEPTM) enables high-coverage proteomic analysis of multiple PTMs from a single biological sample.
The integration of microRNA target sequence features and data from cross-linking and immunoprecipitation of Argonaute proteins, implemented in the hidden Markov model–based framework MUMMIE, provides accurate prediction of microRNA targets.
Contact spotting with standard microarray printing tools can be used to generate high-density arrays of living mammalian cells, permitting the arraying of cell libraries without complex fluid manipulation.
Surface expression of zinc-finger proteins coupled with double-stranded DNA probes permits programmable labeling and capture of subsets of cells in heterogenous populations.
Combining a method to label newly synthesized proteins and a strategy to distinguish two populations in cell culture allows quantitative assessment of intracellular protein dynamics.
A high-sensitivity, high-quality yeast two-hybrid screen using short-read sequencing as its readout is used to map the interactome of human methyltransferases.
An imaging modality that restricts the number of photons detected in each pixel of an electron-multiplying CCD to an average of <1 allows single-fluorophore localization accuracies that approach the ultimate limit.
Gene targeting via homologous recombination is achieved in the zebrafish with TALENs and double-stranded DNA donors, expanding the range of experimental possibilities in this organism.