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A set of targeted mass spectrometry assays for 'sentinel' proteins allows the activation of 188 yeast biological processes to be simultaneously monitored in 1 hour.
A microfluidic chip is used to construct a microarray of proteins, each labeled with a dockerin tag, for high-throughput single-molecule force spectroscopy experiments using a single cohesin-modified cantilever.
An adaptive optics method using multiplexed light measurement and modulation in multiple pupil segments improves structural and functional in vivo imaging over large volumes in strongly scattering mouse brain with only a single aberration correction.
A 'protein quake' is directly monitored on the picosecond timescale using the method of time-resolved wide-angle X-ray scattering at an X-ray free-electron laser.
Single guide RNAs driven by a T7 promoter target Cas9 to two endogenous loci, leading to fast and efficient genome editing in the malaria parasite P. falciparum.
Integration of genome visualization with Bioconductor-based analysis tools allows rapid and interactive analysis of genomes, transcriptomes and epigenomes.
High-resolution, three-dimensional protein structures can be solved using MicroED, an electron diffraction method that uses three-dimensional microcrystals. An improved MicroED data collection approach described here increases data quality and resolution and extends its broad applicability.
To find causative mutations in rare disorders, the Phen-Gen software combines disease symptoms and sequencing data with prior knowledge about the gene.
The compositional heterogeneity of proteoliposome reconstitution can skew the results of ensemble-average measurements of transmembrane protein structure and function. These compositional heterogeneities can be exploited, however, with a single-proteoliposome, high-content screening method.
This paper describes immunocompromised rag2 mutant zebrafish, which allow efficient and robust cell transplantations in adult zebrafish, thereby facilitating stem cell, cancer and regeneration research.
A series of technical and analytical improvements to light sheet microscopy is described, permitting dynamic imaging of the beating zebrafish heart at cellular resolution.
Far-red fluorogenic probes for live-cell imaging of either actin or tubulin are described and used for super-resolution microscopy of various structures in a variety of cell types.
This paper reports the use of light-field microscopy for fast, large-scale imaging of neuronal activity in vivo. It is applied to image the entire animal in the worm and the whole brain in zebrafish.
By combining the use of relatively large crystals and an X-ray free-electron laser, a radiation damage–free three-dimensional structure of a radiation-sensitive protein (bovine cytochrome oxidase) was solved at 1.9-Å resolution.
This paper combines cryo-electron tomography with super-resolution fluorescence microscopy for precise localization of molecular tags on a cellular or macromolecular structure, without fixation.
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9–based transcriptional repressors can be easily engineered to give rise to a large library of orthogonal devices for complex circuits in mammalian cells.