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A statistical method that uses spike-ins to model the dependence of technical noise on transcript abundance in single-cell RNA-seq experiments allows identification of genes wherein observed variability in read counts can be reliably interpreted as a signal of biological variability as opposed to the effect of technical noise.
An algorithm and software tool, Borges, utilizes nonspecific tertiary-structure fragment information available in the Protein Data Bank to phase protein X-ray diffraction data.
The addition of a low percentage of DMSO into liquid chromatography solvents strongly enhances peptide electrospray ionization, substantially improving proteome analysis by liquid chromatography–tandem mass spectrometry.
A proteomic method to identify human proteins post-translationally modified by poly(ADP-ribosyl)ation is reported, which will help yield further insights into the biological role of this modification.
Renewable affinity reagents with high specificity and affinity for histone modifications perform well in ChIP-seq and other applications in epigenetics research.
To determine microbial community structure, the UPARSE software extracts operational taxonomic unit (OTU) representative sequences with high accuracy on the basis of amplified marker-gene sequences.
A combination of allele-specific and non–allele-specific probes allows in situ detection and quantification of mRNA transcripts that differ by only a few SNPs.
A combination of detection probes, targeting a single-nucleotide variant on individual transcripts, and guide probes to diminish the amount of false positives, enables quantification of allele-specific gene expression.
Synchrotron-based Fourier transform infrared (FTIR) spectro-microtomography is a nondestructive, label-free imaging technique that allows chemical fingerprinting of intact, three-dimensional biological samples.
The specI software automatically and highly accurately delineates and assigns bacterial species based on a set of universal marker genes that it extracts from sequenced genomes.
Synthetic transcription factors based on the RNA-guided CRISPR-Cas9 system are used to activate endogenous genes in human cells. Also online, Gersbach and colleagues report similar developments at multiple other loci.
Synthetic transcription factors based on the RNA-guided CRISPR-Cas9 system are used to activate specific endogenous genes in human cells. Also online, Joung and colleagues report similar developments at two other loci.
An RNA aptamer specific for a protein of interest, when fused to an RNA sensor that activates a small-molecule fluorophore, can quantitate protein expression in live bacteria.
A method based on in situ sequencing by ligation enables direct reading of short segments of RNA or sequence tags in preserved tissue sections and cells.
A growing collection of segmented and feature-extracted videos recording locomotive behavior in hundreds of C. elegans mutant strains is made available for phenotyping and further analysis.