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Long-read metagenomics offers a valuable approach for profiling bacterial communities. This work presents a long-read assembler, metaFlye, that specifically addresses the challenges of assembling metagenomes.
MSFragger-Glyco allows identification of N- and O-linked glycopeptides using the localization-aware open search strategy of the MSFragger search engine.
EthoLoop enables real-time tracking and behavioral analysis of animals in naturalistic environments and can be combined with behavioral conditioning, optogenetic stimulation or wireless recording of neural activity. The system is illustrated with freely behaving mice and mouse lemurs.
A genetically encoded acetylcholine sensor with improved sensitivity allows detection of cholinergic neurotransmission in vivo in the Drosophila and mouse brain.
It remains impossible using conventional Hi-C to differentiate interactions between and along sister chromatids. SisterC relies on selective destruction of nascent DNA and in combination with Hi-C offers a means to study intra- and inter-sister interactions independently.
Plasmonic scattering microscopy (PSM) enables the imaging of single proteins on SPR instruments. The method enables measurement of protein size and binding kinetics and is fully compatible with simultaneous traditional SPR measurements.
Tension-PAINT integrates molecular tension probes with DNA-PAINT to enable ~25-nm-resolution mapping of piconewton mechanical events. Tension-PAINT can be used to study dynamic forces, and an irreversible variant integrates force history over time.
Temporal decomposition through manifold fitting (TDM) is an analysis technique that decomposes blood oxygenation level dependent (BOLD) responses in task-based fMRI into different components that likely correspond to microvasculature- and macrovasculature-driven signals.
Red and yellow versions of the genetically encoded dopamine sensor dLight1 have been developed and allow multiplexed imaging of dopamine with neurotransmitter or cell-type-specific calcium combined with green sensors or actuators, as demonstrated ex vivo and in behaving rodents.
ScNT-seq leverages metabolic labeling of nascent RNA and droplet-based sequencing for parallel analysis of newly transcribed and pre-existing mRNAs, which enables time-resolved analysis of dynamic biological processes in single cells.
Scale-free vertical tracking microscopy based on a ‘hydrodynamic treadmill’ enables measuring long-range movements of freely suspended organisms with high spatiotemporal resolution.
Genetically encoded cysteine-rich tags enable formation of gold nanoparticles in situ for single-molecule imaging of individual proteins in the context of cellular ultrastructure in bacterial, yeast and mammalian cells.
Chemically inducible trimerization tools based on split FRB or FKBP with full-length FKBP or FRB, respectively, expand the chemogenetics toolbox. Their efficiency and fast kinetics enable new types of protein manipulation in live cells.
A general tuning strategy is introduced for improving the utility of rhodamines for biological imaging applications. The strategy yielded bright, versatile and bioavailable far-red and near-infrared ‘Janelia Fluor’ dyes.
OligoFISSEQ combines Oligopaints with fluorescence in situ sequencing to enable the 3D mapping of many regions across the genome in human cells to interrogate genome organization at improved genomic resolution. OligoFISSEQ is compatible with immunochemistry and OligoSTORM for super-resolution imaging.