Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
A nanobody-based immunolabeling method, vDISCO, boosts the signal of fluorescent proteins and allows imaging of subcellular details in intact transparent mice. It uncovers neuronal projections and skull–meninges connections in whole adult mice.
Using a deep learning approach to track user-defined body parts during various behaviors across multiple species, the authors show that their toolbox, called DeepLabCut, can achieve human accuracy with only a few hundred frames of training data.
The authors introduce a variant GCaMP that is targeted to axons. Due to its strong brightness, signal-to-noise ratio, and photostability, this tool, named axon-GCaMP, enables robust Ca2+ imaging in individual axons, including in vivo in awake mice.
The authors present a new approach to create and edit custom spatiotemporal neural activity patterns in awake, behaving animals with extremely high spatial and temporal precision. They present novel opsins optimized for multiphoton optogenetics.
Rabies viral vectors are important tools in neuroscience, but their cytotoxicity usually limits their use. Chatterjee et al. introduce a new class of double-deletion-mutant rabies viral vectors that leaves neurons alive and healthy indefinitely.
This study describes single-nucleus ATAC-seq, a method to profile open chromatin in individual nuclei from frozen tissues. It is used to examine gene regulation in 15,000 nuclei comprising 20 distinct cell types in the developing mouse forebrain.
CRISPR interference-based gene silencing was adopted to achieve highly efficient multiple and conditional gene knockdown in the mouse brain with negligible off-target effects, providing a rapid gene interrogation tool in the mammalian brain.
dCas9-mediated activation has been verified and widely used in vitro. Here the authors generated a potent in vivo activation platform and applied it to control the transcription of multiple genetic elements in the mammalian brain.