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Flow Cytometry
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Flow cytometry has been an essential technique in modern biological research. It was originally used almost exclusively for fluorescence-based analysis and sorting of cells labeled with fluorescent antibodies, and this is still the main application. But recently, the number and variety of applications using flow cytometers has been expanding rapidly. This collection of articles highlights recent methodological advances in the use of flow cytometry and the powerful new applications being developed.
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Flow Cytometry collection
Letter
Tumour evolution inferred by single-cell sequencing
Nicholas Navin, Jude Kendall, Jennifer Troge, Peter Andrews, Linda Rodgers, Jeanne McIndoo, Kerry Cook, Asya Stepansky, Dan Levy, Diane Esposito, Lakshmi Muthuswamy, Alex Krasnitz, W. Richard McCombie, James Hicks & Michael Wigler
Nature 472, 90–94 (2011)
doi:10.1038/nature09807
Letter
Sensitive digital quantification of DNA methylation in clinical samples
Meng Li, Wei-dong Chen, Nickolas Papadopoulos, Steven N Goodman, Niels Christian Bjerregaard, Søren Laurberg, Bernard Levin, Hartmut Juhl, Nadir Arber, Helen Moinova, Kris Durkee, Kerstin Schmidt, Yiping He, Frank Diehl, Victor E Velculescu, Shibin Zhou, Luis A Diaz Jr, Kenneth W Kinzler, Sanford D Markowitz & Bert Vogelstein
Nature Biotechnology 27, 858–863 (2009)
doi:10.1038/nbt.1559
Article
Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression
Marlon Stoeckius, Jonas Maaskola, Teresa Colombo, Hans-Peter Rahn, Marc R Friedländer, Na Li, Wei Chen, Fabio Piano & Nikolaus Rajewsky
Nature Methods 6, 745–751 (2009)
doi:10.1038/nmeth.1370
Article
Knocking out multigene redundancies via cycles of sexual assortment and fluorescence selection
Yo Suzuki, Robert P St Onge, Ramamurthy Mani, Oliver D King, Adrian Heilbut, Vyacheslav M Labunskyy, Weidong Chen, Linda Pham, Lan V Zhang, Amy H Y Tong, Corey Nislow, Guri Giaever, Vadim N Gladyshev, Marc Vidal, Peter Schow, Joseph Lehár & Frederick P Roth
Nature Methods 8, 159–164 (2011)
doi:10.1038/nmeth.1550
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