Focus on deep imaging of live tissue
One of the goals of biological imaging is to watch biological processes where they occur – within living tissue or even within a living animal. But in vivo imaging presents a set of challenges that are not encountered when imaging relatively small, flat samples like cells. In this Focus issue, we bring together papers on methods for optical imaging within living tissue. Two Reviews discuss: microscopy methods for functional brain imaging and the principles and practicalities of the different flavours of light sheet microscopy. A Perspective describes the use of adaptive optics to correct aberrations in scattering samples. This issue also includes primary research papers on multiphoton microscopy, an editorial that reminds our readers of the many areas of this large and exciting field that we do not cover, and a collection of recent papers on deep imaging from Nature Research journals.
