Abstract
Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
Design Type(s) | cell organelle imaging objective |
Measurement Type(s) | X-ray diffraction data |
Technology Type(s) | X-ray free electron laser |
Factor Type(s) | |
Sample Characteristic(s) | Halothiobacillus neapolitanus • carboxysome |
Machine-accessible metadata file describing the reported data (ISA-Tab format)
Similar content being viewed by others
Background & Summary
X-ray free-electron lasers generate ultra-short and extremely bright coherent X-ray pulses. Such pulses can be used to produce diffraction patterns from a single particle before it is destroyed due to radiation-induced processes1–3, as demonstrated in recent experiments2,4–6. Sample heterogeneity, inherent in biology, interferes with crystallisation leading to systematic gaps of knowledge in structural biology. This prevents most large biological entities (e.g., cell organelles, large virus particles, flexible protein complexes) from being crystallized, and even when crystallization is successful only the reproducible regions can be resolved. Flash X-ray imaging (FXI) does not rely on crystallisation and thus has the potential to study structures of single particles and biomolecules that exhibit heterogeneity. High-throughput FXI is needed to measure three-dimensional structures, to achieve high-resolution and may even allow solving flexible structures. It has been shown that the concept of ‘diffraction before destruction’1 gives a significant advantage for resolving radiation sensitive material7,8. Although simple in concept, collecting sufficient high-quality diffraction patterns and separating the different structures present in the data is challenging.
We publish here an FXI dataset on aerosolised particles from a suspension of carboxysomes that were purified from the proteobacterium Halothiobacilus neapolitanus. This is the same data set used in the publication of Hantke et al.5. Carboxysomes are polyhedral cell organelles that vary in size and in shape (Fig. 1a). The purified carboxysomes were complementary studied prior to injection by single-particle tracking analysis and electron microscopy (Fig. 1a). The size range is wide and peaks at 115 nm and the carboxysomes share an icosahedral appearance.
The experimental design is depicted in Fig. 1b,c. Aerosolised carboxysomes are injected into the X-ray pulse train of the Linac Coherent Light Source (LCLS) and diffraction patterns of isolated particles are recorded with an area detector. The direct beam passes through a narrow gap between the two detector halves and is absorbed by a beam dump behind the detector.
Methods
These methods are expanded from the previous descriptions in ref. 5.
Sample preparation
Carboxysomes were purified from Halothiobacillus neapolitanus 15147 cells. Cells were broken up by sonication and the debris pelleted at 12,000 g. The supernatant containing carboxysomes was centrifuged at 40,000 g and the pellet was re-suspended in a 1:1 mixture of TEMB buffer (10 mM Tris-HCl, 10 mM MgCl2, 20 mM NaHCO3, 1 mM EDTA, pH 8.0) and B-PERII (Thermo Scientific). The centrifugation and resuspension steps were repeated twice. The final pellet was resuspended in TEMB and filtered through a 0.2 μm membrane. Carboxysomes were transferred to 20 mM ammonium acetate (pH 7.5), using a PD MiniTrap G-25 column (GE Healthcare), shortly before experiments at the LCLS.
Injection
An aerosol injector9 designed by our group in Uppsala4,5 was used to introduce free-flying particles into the pulse train of the X-ray laser at a reduced pressure. Purified carboxysomes were transferred into a volatile buffer (20 mM ammonium acetate, pH 7.5) and were aerosolised with helium, using a gas dynamic virtual nozzle10. The sample consumption was 2–4 μlmin−1 from a solution of 1.2×1011 particles per ml. The aerosol entered the injector via an inlet nozzle coupled to a skimmer. Excess nebulising gas was pumped away at this stage. The concentrated aerosol passed through a relaxation chamber from where the adiabatically cooled particles entered an aerodynamic lens4,11,12. The injected sample volume was 36 μl.
Diffraction
The experiment was carried out with the CAMP instrument13,14 at the Atomic, Molecular and Optics (AMO) end-station15,16 of the Linac Coherent Light Source17 (LCLS). A sketch of the experimental set up with the most important parameters is depicted in Fig. 1c. LCLS delivered X-rays at 1.10 keV (1.13 nm wavelength) in pulses of 120 fs full-duration at half maximum. For the photon energy the band width was 1% and the standard deviation between shots was 0.002 keV. The X-ray beam was focused to a spot of about 5 μm full-width at half maximum, giving about 6.8×1010 photons per μm2 in the center of the beam, assuming a Gaussian beam profile. The particle beam with a diameter of about 15 μm and a particle flux of 108 particles per minute was intersected with the X-ray laser beam. Diffraction patterns were recorded with a pnCCD area detector14 that was positioned at a distance of 741 mm from the interaction region. The detector consists of two panels with 512×1,024 pixels each. The direct beam passes through a gap of 3.7 mm between the panels. The edge length of a single pixel is 75 μm. The detector was operated at low gain with high-charge handling capacity. Seven analog-to-digital units (ADU) measure one photon. The quantum efficiency of the detector at the given photon energy is 0.88. LCLS and the pnCCD detectors were running at 120 Hz and data were recorded over 12 min non-stop.
Data pre-processing
Data were pre-processed with the Cheetah software package18. The 'pedestal-corrected raw data' consist of minimally processed data with dark frame subtraction. Electronic background was removed by subtraction of the average value of 4,834 dark exposures that were recorded prior to the 12 minute-long measurement. The generation of 'pre-processed data' includes labelling and masking of pixels, compensation of detector artefacts, pixel arrangement according to the experimental geometry, persistent photon background subtraction and hit finding. Defective pixels and hot pixels were masked out. Pixels that measured high signal had a non-linear detector response, and were adjusted with an empirical formula derived from measurements with known illumination. Persistent contributions of the photon background were suppressed by subtracting the median pixel values gathered from the ‘misses’ (i.e., non-hits). Pixels that were exposed to a highly fluctuating photon background (standard deviation greater than 3 photons) were masked out. The 'pre-processed data' is immediately useable with minimum additional effort.
Hit finding
A down-sampled pixel was considered ‘lit’ if it contained more than 36 photons. Patterns in which at least 400 8-by-8 down-sampled pixels were lit were considered hits. For defining this threshold diffraction patterns were sorted according to the number of ‘lit’ pixels. Sequences of 100 diffraction patterns in this sorted array were manually inspected and the hit ratios in these subsets were determined (see Fig. 2). The error function was fitted to the data points and the hit threshold was set to the number of lit pixels at the inflection point.
Data Records
Extracted raw and pre-processed diffraction data are saved to the file amo55912-r0121.cxi in the Hierarchical Data Format version 5 (HDF5, http://hdfgroup.org). The data layout is documented at the Coherent X-ray Imaging Data Bank19 (CXIDB). The three main data sets are described in Table 1. Data set /entry_1/data_1/data contains the raw data. Pre-processed data are provided in data set /entry_1/image_1/data. The arrangement of the 2-dimensional data array corresponds to the true physical arrangement, which results in the slightly larger array size compared to the raw data. Due to the high sampling ratio of the diffraction patterns we also provide the data set /entry_1/image_2/data, which contains pre-processed frames that were down-sampled 4 by 4.
All files of data record 1 are listed in Table 2. In addition to extracted and pre-processed data we also provide the original files in the eXtended Tagged Container (XTC) format that contain all data recorded during the experiment. We provide also the calibration and configuration files that were used to extract data from the XTC files with the Cheetah software package18 (git commit 43b4cf5).
The data is deposited on the CXIDB under entry ID 25 (Data Citation 1).
Technical Validation
We show in Fig. 3a the diffraction pattern of a single carboxysome and in Fig. 3b a detector read out from a blank shot that demonstrates high ratio of signal to noise in the data. In Fig. 3c we show a subset of diffraction patterns and the distribution of the brightness of the diffraction patterns. We find that from about 87,000 recorded exposures about 70,000 were hits on at least one particle. Most hits were faint. In the size domain expected for our carboxysomes (100–130 nm) many objects showed straight edges, resembling icosahedra. The predominant fraction of objects in the data set are round. Such round objects are probably some other material of similar density; carboxysomes were purified on the basis of their size and density and impurities can be expected. Nevertheless, we can separate the different structures from each other computationally5.
A first analysis on these data5 demonstrated sorting of the diffraction patterns and automatic reconstruction of projection images. The analysis reproduced the size distribution observed in solution (Fig. 4a). In the reconstructions of projection images from individual diffraction patterns phases were retrieved up to a full-period resolution of 18.2 nm. Multiple hits can be discerned from single hits from the autocorrelation function (Fig. 4b).
Figure 5 shows the effect of the missing data regions. This was estimated for 115-nm-sized particles with a missing mode analysis tool4,20. The four least constrained modes have singular values of 0.862, 0.606, 0.520 and 0.509 respectively (Fig. 5a, top). Assuming uniform noise the missing data corresponds to an increase of the noise level by a factor of 7.27, 2.54, 2.08, and 2.04 for the respective modes (Fig. 5a, bottom). Due to the fact that these least constrained modes are most prominent in the centre of the pattern (Fig. 5b) where the signal-to-noise ratio is high suggests that the effect of the missing data is negligible.
Additional Information
How to cite this article: Hantke, M. F. et al. A data set from flash X-ray imaging of carboxysomes. Sci. Data 3:160061 doi: 10.1038/sdata.2016.61 (2016).
References
References
Neutze, R., Wouts, R., van der Spoel, D., Weckert, E. & Hajdu, J. Potential for biomolecular imaging with femtosecond X-ray pulses. Nature 406, 752–757 (2000).
Chapman, H. N. et al. Femtosecond diffractive imaging with a soft-X-ray free-electron laser. Nat. Phys 2, 839–843 (2006).
Bergh, M., Huldt, G., Timneanu, N., Maia, F. R. N. C. & Hajdu, J. Feasibility of imaging living cells at subnanometer resolutions by ultrafast X-ray diffraction. Q. Rev. Biophys. 41, 181–204 (2008).
Seibert, M. M. et al. Single mimivirus particles intercepted and imaged with an X-ray laser. Nature 470, 78–U86 (2011).
Hantke, M. F. et al. High-throughput imaging of heterogeneous cell organelles with an X-ray laser. Nat. Photonics 8, 943–949 (2014).
van der Schot, G. et al. Imaging single cells in a beam of live cyanobacteria with an X-ray laser. Nat. Commun 6, 5704 (2015).
Johansson, L. C. et al. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. Nat. Commun 4, 1–7 (2013).
Kern, J. et al. Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature. Science 340, 491–495 (2013).
Bogan, M. J. et al. Single particle X-ray diffractive imaging. Nano Lett 8, 310–316 (2008).
DePonte, D. P. et al. Gas dynamic virtual nozzle for generation of microscopic droplet streams. J. Phys. D. Appl. Phys 41, 195505 (2008).
Murphy, W. K. & Sears, G. W. Production of Particulate Beams. J. Appl. Phys. 35, 1986 (1964).
Bogan, M. J. et al. Single particle X-ray diffractive imaging. Nano Lett. 8, 310–316 (2008).
Epp, S. W. et al. CAMP—A new endstation for simultaneous detection of photons and charged particles in free electron lasers experiments. J. Phys. Conf. Ser 194, 142017 (2009).
Strüder, L. et al. Large-format, high-speed, X-ray pnCCDs combined with electron and ion imaging spectrometers in a multipurpose chamber for experiments at 4th generation light sources. Nucl. Instruments Methods Phys. Res. Sect. A Accel. Spectrometers, Detect. Assoc. Equip 614, 483–496 (2010).
Bozek, J. D. AMO instrumentation for the LCLS X-ray FEL. Eur. Phys. J. Spec. Top 169, 129–132 (2009).
Bostedt, C. et al. Ultra-fast and ultra-intense x-ray sciences: first results from the Linac Coherent Light Source free-electron laser. J. Phys. B At. Mol. Opt. Phys 46, 164003 (2013).
Emma, P. et al. First lasing and operation of an ångstrom-wavelength free-electron laser. Nat. Photonics 4, 641–647 (2010).
Barty, A. et al. Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data. J. Appl. Crystallogr 47, 1118–1131 (2014).
Maia, F. R. N. C. The Coherent X-ray Imaging Data Bank. Nat. Methods 9, 854–855 (2012).
Thibault, P., Elser, V., Jacobsen, C., Shapiro, D. & Sayre, D. Reconstruction of a yeast cell from X-ray diffraction data. Acta Crystallogr. Sect. A 62, 248–261 (2006).
Data Citations
Hantke, M. F. Coherent X-ray Imaging Data Bank http://dx.doi.org/10.11577/1169545 (2014)
Acknowledgements
This work was supported by the Swedish Research Council, the Knut and Alice Wallenberg Foundation, the Swedish Foundation for Strategic Research, the European Research Council, the Röntgen-Ångström Cluster, Stiftelsen Olle Engkvist Byggmästare, the Ministry of Education, Youth and Sports of the Czech Republic (ELI-Beamlines Registered No. CZ.1.05/1.1.00/02.0061), the Academy of Sciences of the Czech Republic (M100101210), and EMBO ALTF 1531–2010. Portions of this research were carried out at the Linac Coherent Light Source, a national user facility operated by Stanford University on behalf of the US Department of Energy, Office of Basic Energy Sciences. The authors thank the scientific and technical staff of the LCLS for support. The authors thank the CAMP collaboration for giving access to their experimental set-up and for supporting the experiment at the LCLS. The authors also acknowledge the Max Planck Society for funding the development and operation of the CAMP instrument.
Author information
Authors and Affiliations
Contributions
M.F.H., F.R.N.C.M., T.E., and J.H., developed the imaging concept and conceived the experiment. M.F.H., F.R.N.C.M., T.E., A.B., N.D.L., A.M., G.V.D.S., and D.L. developed ideas and software to process the diffraction data. D.H., K.J., G.H.C., M.S., M.I., and I.A. prepared and characterized carboxysomes for the study. J.H., B.I., J.B., C.U., D.P.D., R.A.K., M.S., J.A., M.M.S., D.W., and S.B. developed and operated the sample injector. J.D.B., C.B., S.C., L.F., N.T., M.S., and M.M.S. operated the beamline at the LCLS. R.H., and N.K. operated the pnCCD detectors. M.F.H., F.R.N.C.M., T.E., N.D.L., G.V.D.S., A.B., J.A., M.M.S., M.S., M.L., F.S., D.R., A.R., S.W.E., H.N.C., and J.H. characterized the imaging apparatus and carried out the experiment. M.F.H., F.R.N.C.M., and T.E. processed the data. M.F.H., J.H., and F.R.N.C.M. analysed the results and wrote the manuscript with input from the others.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
ISA-Tab metadata
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
About this article
Cite this article
Hantke, M., Hasse, D., Ekeberg, T. et al. A data set from flash X-ray imaging of carboxysomes. Sci Data 3, 160061 (2016). https://doi.org/10.1038/sdata.2016.61
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/sdata.2016.61
This article is cited by
-
Microfluidic sample delivery for serial crystallography using XFELs
Analytical and Bioanalytical Chemistry (2019)
-
Coherent diffractive imaging of microtubules using an X-ray laser
Nature Communications (2019)
-
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene
Nature Communications (2018)
-
The trickle before the torrent—diffraction data from X-ray lasers
Scientific Data (2016)