Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

CATALYTIC DNA

How DNA catalyses RNA ligation

Nature Catalysis volume 2, pages 483–484 (2019)Cite this article

Subjects

A synthetic DNA enzyme catalyses the formation of a native phosphodiester bond between two RNA fragments, but the molecular details of the mechanism remained elusive. Research using computational and biochemical approaches now suggests that the DNA enzyme recruits two magnesium ions to assist in the catalysis of RNA ligation.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: DNA-catalysed RNA ligation.

References

  1. Joyce, G. F. Angew. Chem. Int. Ed. 46, 6420–6436 (2016).

    Article  Google Scholar 

  2. Breaker, R. R. & Joyce, G. F. Chem. Biol. 1, 223–229 (1994).

    Article  CAS  Google Scholar 

  3. Purtha, W. E., Coppins, R. L., Smalley, M. K. & Silverman, S. K. J. Am. Chem. Soc. 127, 13124–13125 (2005).

    Article  CAS  Google Scholar 

  4. Ponce-Salvatierra, A., Wawrzyniak-Turek, K., Steuerwald, U., Höbartner, C. & Pena, V. Nature 529, 231–234 (2016).

    Article  CAS  Google Scholar 

  5. Aranda, J.,Terrazas, M., Gomez, H., Villegas, N. & Orozco M. Nat. Catal. https://doi.org/10.1038/s41929-019-0290-y (2019).

  6. Raper, A. T., Reed, A. J. & Suo, Z. Chem. Rev. 118, 6000–6025 (2018).

    Article  CAS  Google Scholar 

  7. Mattioli, E. J., Bottoni, A. & Calvaresi, M. J. Chem. Inf. Model. 59, 1547–1553 (2019).

    Article  CAS  Google Scholar 

  8. Wachowius, F. & Höbartner, C. J. Am. Chem. Soc. 133, 14888–14891 (2011).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Organic Chemistry, Julius-Maximilians-University Würzburg, Würzburg, Germany

    Claudia Höbartner

Authors
  1. Claudia Höbartner
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Claudia Höbartner.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Höbartner, C. How DNA catalyses RNA ligation. Nat Catal 2, 483–484 (2019). https://doi.org/10.1038/s41929-019-0295-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41929-019-0295-6

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing