Correction to: Nature Protocols https://doi.org/10.1038/s41596-022-00681-y. Published online 23 March 2022.

In the version of this article initially published, the fifth image in the 5.0 ng/mL column of Fig. 6b was a duplicate of the second image in that column, and in Fig. 6c, the third image in the SOX2 column was a duplicate of the second image; the figure has been updated. Figure callouts on pages 1273, 1276, 1279, 1300 and 1302 were incorrect and are now updated. The “Recombinant human BMP4” row of Table 6 showed an incorrect vendor (R&D Systems) and catalog number; the entry is updated to read “PeproTech, cat. no. 120-05”. In Table 7, in the “Working concentration” (originally “Final concentration”) column for “Recombinant human bFGF,” the text “(50 ng/mL 5×)” originally appeared in the “Stock concentration” column. On page 1280, catalog numbers for BSA, PBS, citric acid, HSA and DMSO have been updated, as well as the vendor for DMSO, and on page 1282, the vendor and catalog number for parafilm and the catalog number for the 0.22 μm filter have been updated. In the Fig. 3 legend, text has been corrected to say that the periderm is visible in organoids around days 40–75 (not 25–60 as originally stated). The changes have been made in the HTML and PDF versions of the article.