Eukaryotes possess several clamp loaders comprising four common subunits and a fifth subunit unique to each complex. The RFC-A–E clamp loader loads the PCNA clamp at 3′-recessed structures for DNA replication. However, swapping a single subunit, Rad24, for RFC-A yields a clamp loader that prefers the 911 clamp and 5′-recessed DNA. Three new studies reveal detailed views of the clamp loading reaction and provide insights into substrate preferences of each loader.
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Jeruzalmi, D. The partner-swapping sliding clamp loader exposed. Nat Struct Mol Biol 29, 283–286 (2022). https://doi.org/10.1038/s41594-022-00761-3
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DOI: https://doi.org/10.1038/s41594-022-00761-3