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RNA sequencing

Mapping mRNA modifications for functional studies

Nature Biotechnology volume 41, pages 324–325 (2023)Cite this article

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New methods for mapping the mRNA modifications N6-methyladenosine and pseudouridine enable large-scale functional analyses.

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Fig. 1: Transcriptome-wide measurements of Ψ and m6A mRNA modification stoichiometry.

References

  1. Jonkhout, N. et al. RNA 23, 1754–1769 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Liu, C. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01487-9 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  3. Dai, Q. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01505-w (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  4. Khoddami, V. et al. Proc. Natl Acad. Sci. USA 116, 6784–6789 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Moshitch-Moshkovitz, S., Dominissini, D. & Rechavi, G. Cell 185, 764–776 (2022).

    Article  CAS  PubMed  Google Scholar 

  6. Kierzek, E. et al. Nucleic Acids Res. 42, 3492–3501 (2014).

    Article  CAS  PubMed  Google Scholar 

  7. Yeo, J., Goodman, R. A., Schirle, N. T., David, S. S. & Beal, P. A. Proc. Natl Acad. Sci. USA 107, 20715–20719 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Higuchi, M. et al. Cell 75, 1361–1370 (1993).

    Article  CAS  PubMed  Google Scholar 

  9. Eyler, D. E. et al. Proc. Natl Acad. Sci. USA 116, 23068–23074 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Karijolich, J. & Yu, Y.-T. Nature 474, 395–398 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Michigan, Ann Arbor, MI, USA

    Joshua D. Jones, Daniel E. Eyler & Kristin S. Koutmou

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  1. Joshua D. Jones
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  2. Daniel E. Eyler
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  3. Kristin S. Koutmou
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Correspondence to Kristin S. Koutmou.

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The authors declare no competing interests.

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Jones, J.D., Eyler, D.E. & Koutmou, K.S. Mapping mRNA modifications for functional studies. Nat Biotechnol 41, 324–325 (2023). https://doi.org/10.1038/s41587-022-01537-2

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