Abstract
The intimate relationship between the epithelium and immune system is crucial for maintaining tissue homeostasis, with perturbations therein linked to autoimmune disease and cancer1,2,3. Whereas stem cell-derived organoids are powerful models of epithelial function4, they lack tissue-resident immune cells that are essential for capturing organ-level processes. We describe human intestinal immuno-organoids (IIOs), formed through self-organization of epithelial organoids and autologous tissue-resident memory T (TRM) cells, a portion of which integrate within the epithelium and continuously survey the barrier. TRM cell migration and interaction with epithelial cells was orchestrated by TRM cell-enriched transcriptomic programs governing cell motility and adhesion. We combined IIOs and single-cell transcriptomics to investigate intestinal inflammation triggered by cancer-targeting biologics in patients. Inflammation was associated with the emergence of an activated population of CD8+ T cells that progressively acquired intraepithelial and cytotoxic features. The appearance of this effector population was preceded and potentiated by a T helper-1-like CD4+ population, which initially produced cytokines and subsequently became cytotoxic itself. As a system amenable to direct perturbation, IIOs allowed us to identify the Rho pathway as a new target for mitigation of immunotherapy-associated intestinal inflammation. Given that they recapitulate both the phenotypic outcomes and underlying interlineage immune interactions, IIOs can be used to study tissue-resident immune responses in the context of tumorigenesis and infectious and autoimmune diseases.
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Main
Organoids originating from adult stem cells model important aspects of human physiology and have applications in research related to genetic disorders, infectious disease, cancer, regenerative medicine and drug discovery4. However, they are epithelial-only structures, whereas native organs comprise multiple other compartments, including specialized immune cells that have essential roles in homeostasis and disease5,6. For example, the intestinal mucosal immune system—the largest pool of immune cells in the human body7—ensures homeostasis by perpetual policing of the interface between the intestinal barrier and luminal contents. Disruption of intestinal immune function can lead to various pathologies, including persistent infections and autoimmune and even malignant disease1,2,3,8. Whereas intestinal organoids can accurately model the differentiation and function of epithelial cell types9,10,11, they fall short in capturing key aspects of intestinal (patho)physiology owing to the absence of a tissue-specific immune compartment12.
To address these shortcomings, adult or induced pluripotent stem cell-derived organoids have been co-cultured with blood-derived innate or adaptive immune cells13,14,15. However, the incorporation of a mucosal lymphocyte compartment has proven elusive. One recent study described the generation of gut-associated lymphoid tissue in induced pluripotent stem cell-derived human intestinal organoids following transplantation in humanized mice16. Although representing an important advance, the in vivo formation of immune tissue removes the advantage of organoids as being controllable in vitro systems. Similarly, fetal intestinal organoids have been co-cultured with effector memory T cells but no model exists that includes a true adult tissue-resident lymphocyte compartment with the memory features acquired over the course of a human lifespan17.
We created a tractable intestinal immuno-organoid (IIO) model containing a tissue-resident and autologous immune compartment starting from readily available adult human tissue samples (Fig. 1a). We benchmarked cell states in IIOs through comparison with reference atlases using single-cell transcriptomes, and used IIOs to recapitulate and investigate drug-induced intestinal inflammation.
a, Schematic overview of establishment of autologous IIOs (adapted with permission from ref. 60). b,c, Flow cytometry-based, t-distributed stochastic neighbour-embedding (t-SNE) analysis of donor-matched gut TRM cell and circulating PBMC T cell subgroups based on surface marker expression, derived from one donor and representative of six biological replicates. t-SNE plot coloured according to original source of T cells (b; light grey for PBMCs and black for TRM cells), and expression of ten individual markers of naivety, memory and tissue residency (c). d, Fluorescent live image 24 h following IIO co-culture set-up (nuclei, teal; T cells, pink). Similar images were observed with four biological replicates. e–g, mIF staining of cultures 24 h following co-culture with autologous TRM cells (e,f) or PBMCs (g). Intestinal organoids highlighted by E-cadherin+ (ECAD) epithelium (red). CD4+ (green) and CD8+ (turquoise) TRM cells integrated within larger (e) or smaller (f) organoids whereas blood-derived CD4+ and CD8+ T cells surrounded the organoid (g). Box i and box ii highlight the presence (f) or absence (g) of immune cell integration into two representative regions (i and ii) of the epithelium. e–g, miF images representative of three independent biological replicates. IO, intestinal organoid. h, Immune cell count detected per organoid, each data point representing an individual organoid; n = 18 for PBMCs and n = 54 for TRM cells, two-tailed Mann–Whitney test. i, Ratio of epithelial to immune cells within each organoid (n = 35), 24 h following organoid supplementation with autologous TRM cells. h,i, Data represent the collation of two independent IIO cultures. Similar results were observed in three biological replicates. j, Elongated flossing T cell inserting between basal and lateral epithelial cell junctions. Scale bars, 25 µm (d–g, j).
In our efforts to introduce a relevant lymphocyte compartment into organoids, we focus on tissue-resident memory T (TRM) cells—antigen-experienced T cell populations, which take permanent residence in the intestinal mucosa, providing frontline defence against pathogens6. Given the absence of recirculation, they are an appropriate lymphocyte type for stable incorporation into organoid models. Furthermore, their memory character ensures functionality in the absence of antigen-presenting cells, lymphoid structures and the remaining immune cell recirculation apparatus. Because human TRM cells are difficult to incorporate into in vitro systems owing to their poor viability following enzymatic removal from tissue18,19, we adapted an enzyme-free, scaffold-based crawl-out protocol to isolate large numbers of intestinal immune cells20 (Fig. 1a). Even in the complete absence of cytokine or T cell receptor (TCR) stimulation, our approach liberated significantly more cells than enzymatic digestion-based protocols, meanwhile retaining similar proportions of immune cell types (Extended Data Fig. 1a,b). Flow cytometry analysis showed that the isolated cells expressed TRM cell markers pertinent to the intestine, including CD161 (IL-17A production21) and CD117 (Notch signalling22, encoded by KIT; Extended Data Fig. 1c), as well as surface molecules associated with tissue retention (CD69 (ref. 23)), extracellular matrix association (CD49a24, encoded by ITGA1) and epithelial cell integration (CD103 (ref. 25), encoded by ITGAE), all of which were barely detectable on blood-derived T cells (Fig. 1b,c). We deemed the lack of cytokine exposure crucial for retaining the tissue-like physiological properties of intestine-derived lymphocytes, as demonstrated by a change in key activation markers following exposure to IL-2 and IL-15 (Extended Data Fig. 1d). Our gut-derived T cells were exclusively memory cells (CD45RO+CD45RA−) with limited capacity for lymph node homing (CCR7−), and these expressed high levels of the IL-7 receptor CD127 (Extended Data Fig. 1e). The final pool of isolated immune cells sometimes contained myeloid cells and B lymphocytes, largely dependent on the tissue specimen itself (Extended Data Fig. 1b). Nonetheless, because the overwhelming majority (mean 84.5%) were TRM cells, we use the abbreviation TRM cells to refer to the pool of immune cells harvested from human intestinal samples.
We generated organoids and TRM cells from human intestinal specimens, additionally collecting matched peripheral blood mononuclear cells (PBMCs) from the same donor. Once established, organoids were combined with TRM cells or PBMCs within a three-dimensional extracellular matrix (ECM) at physiologically relevant cellular concentrations26 and in the absence of external stimulation. Confocal microscopy showed that, following co-culture, TRM cells were viable and closely associated with organoids (Fig. 1d and Supplementary Video 1). To examine organoid–TRM cell interactions in detail we generated histological sections of the models and visualized both epithelial and immune cells (Fig. 1e–g). We found that, whereas PBMCs occupied the ECM space with no apparent interaction with epithelial cells (Fig. 1g), a subpopulation of TRM cells infiltrated the organoids and integrated within the epithelial barrier in the absence of stimulation, resembling the behaviour of intestinal intraepithelial lymphocytes (IELs)27 (Fig. 1e,f,h). We estimated a median integration ratio of 16 epithelial cells per one immune cell (Fig. 1i)—highly similar to observations in the intestinal tract of healthy humans28. IELs showed an elongated morphology of up to around 60 µm in length (Fig. 1j and Extended Data Fig. 1f), around ten times the length of a naive blood-derived T cell29 and reminiscent of the ‘flossing’ behaviour described for murine IELs imaged in vivo27. With low-level cytokine support, immune-organoid cultures could be maintained for at least 14 days (Extended Data Fig. 1g–j). This model provides an example of self-organization between human immune cells and epithelial organoids to form an organoid system with a tissue-resident immune compartment. We termed these IIOs; henceforth we use IIOs to refer to organoids co-cultured with autologous gut-derived TRM cells and containing integrated IELs.
Elegant in vivo studies have dissected the dynamics of murine TCRγδ IELs and their interaction with the intestinal epithelium27. Owing to their poor survival in vitro, similar studies of human IEL behaviour have proved challenging18,19 and the mechanisms driving human-specific IEL integration are poorly elucidated. To understand how TRM cells and IELs integrate with intestinal epithelial cells in vitro, and how they differ from PBMCs in that regard, we used single-cell RNA sequencing (scRNA-seq) for analysis of IIO cultures derived from three different small intestinal samples. We ran scRNA-seq 24 h following IIO initiation because the integration of TRM cells is complete at this time point, as demonstrated by longitudinal flow cytometry experiments showing stable TRM cell composition and activation profiles within the first 4 days of co-culture, in the absence of any treatment (Extended Data Fig. 2a). We compared these with matched PBMCs co-cultured with organoids, and included gut-derived TRM cells cultured in Matrigel alone as an additional control (Fig. 2a). Heterogeneity analysis and visualization using uniform manifold approximation and projection (UMAP) embedding demonstrated the presence of 10 epithelial and 14 immune cell clusters across all three donors (Extended Data Fig. 2b–e). Epithelial cell proportions and gene expression were generally stable between IIOs and organoids cultured with PBMCs, although we observed that the presence of TRM cells led to a significant upregulation of ID3 and HES1 in IIO epithelial stem cells (Extended Data Fig. 2f,g), genes associated with intestinal stem cell maintenance30,31 and microbial defence32. We focused our analysis on T cells (and closely related natural killer (NK) cells), given their predominance within the tissue-derived population, and identified three distinct populations representing 15 clusters of blood-derived naive, blood- and gut-derived effector cells and gut-derived TRM cells (Fig. 2b,c, Extended Data Fig. 2h and Supplementary Table 1). Further interrogation of T cell populations based on previously published markers demonstrated that TRM cells cultured alone or within IIOs, unlike their matched blood-derived counterparts, were transcriptomically defined by: (1) the absence of receptors necessary for lymph node homing (SELL, CCR7), (2) intrinsically high expression of intestinal homing and integration factors (ITGA1, CCR9, JAML) and (3) a complete absence of cytotoxic granules (GZMB, GZMK, GNLY) (Fig. 2c and Supplementary Table 2). We observed representation across each cluster from all three donors, demonstrating the consistency and reproducibility of the different immune states (Fig. 2d). To identify the main functional differences between TRM cells and PBMCs we considered the top differentially regulated TRM cell genes (Fig. 2e). Gene Ontology analysis suggested an enrichment in transcriptomic programs related to immune cell chemotaxis and migration within TRM cells compared with PBMCs (Fig. 2f), which may explain their propensity for integration within the epithelium. Indeed, live-imaging experiments in which donor-matched gut TRM cells and blood T cells were tracked over time (Fig. 2g and Supplementary Videos 2 (TRM cells) and 3 (blood T cells)) showed a marked difference in morphology and migratory behaviour between the two populations. Whereas blood T cells were largely static and round (Supplementary Video 3), TRM cells exhibited elongated shapes and migrated dynamically within both the epithelial layers and ECM (Fig. 2g,h and Supplementary Video 2). We note that intestinal organoids are a sterile system and that the introduction of luminal microorganisms may lead to altered modes of migration and interaction with the epithelium, as described in mice27.
a, Overview of single-cell transcriptome analysis of organoids containing patient-matched TRM cells and PBMCs. Data were generated from TRM cells alone (TRM), organoids containing TRM cells (IIO) and organoids containing PBMCs (PBMC) from three small intestine donors (one jejunum, one duodenum, one undefined small intestine). Organoid schematic adapted with permission from ref. 60, human schematic adapted from ref. 61. b, Integrated UMAP embedding of transcriptome data from immune cells of each condition, showing 15 distinct cell clusters (colours, numbers) labelled based on analysis of marker genes (see Extended Data Fig. 2 for the entire dataset). R, resting. c, Dotplot summarizing marker gene expression across different clusters. Heatmap sidebar shows proportional distribution of each condition across clusters. d, Left, immune cell UMAP with each cell coloured by donor; right, stacked barplot showing donor cell proportion in each cluster in which each column sums to 100. e, Heatmap representing genes with enriched expression in PBMCs (left) or TRM cells (right). f, Plot showing significantly enriched Gene Ontolology biological processes of genes marking CD8+ and CD4+ TRM cell clusters. Enrichment P value was generated using hypergeometric test with Bonferroni’s multiple-hypothesis correction. g, Images showing migration analysis derived from IIO time-lapse imaging. Tracks are shown in cyan, immune cells in red. Data quantified in h. h, Comparison of TRM cell and blood-derived T cell shape (top) and speed (bottom) from 42 min time-lapse imaging of IIO and organoid + T cell cultures. Two-tailed Mann–Whitney test. Each dot represents a detected cell and is the collation of duplicate cultures from one representative experiment. Cell shape eccentricity, n = 471 for gut TRM cells and n = 593 for blood T cells; motility, n = 2,335 for gut TRM cells and n = 866 for blood T cells. Similar results were observed with three biological replicates. Scale bar, 100 µm.
For assessment of the impact of the epithelium on TRM cells within IIOs, relative to TRM cells cultured alone, we reconstructed a neighbourhood enrichment score (integration index) of T cells based on connectivity in canonical correlation analysis space. Comparison of neighbourhoods composed almost exclusively of IIO-derived TRM cells (bin 5) with those composed of TRM cells cultured alone (bin 3) demonstrated enriched expression of genes associated with cell motility and cytoskeletal rearrangement (ANXA1, ANXA2, LGALS3, S100A6, S100A10, S100A11) (Extended Data Fig. 2i–k). Conversely, TRM cells cultured alone showed heightened expression of genes required to navigate into the gut and around the ECM (JAML, SOS1, CD96, ITGA1), but not of those to integrate into the epithelium (Extended Data Fig. 2k). This indicated that the epithelium reinforced and amplified the intraepithelial character of TRM cells, presumably through the frequent cell-to-cell contacts we observed and describe in Fig. 1.
Next we tested whether differential transcriptomes and migration behaviours between TRM cells and PBMCs would translate into differences in effector function. In particular, we investigated whether IIOs could recapitulate clinical toxicities associated with cancer immunotherapy manifesting as severe intestinal inflammation33,34. We focused on solitomab—a bispecific T cell engager intended to cross-link activated T cells with solid tumours via the epithelial cell adhesion molecule (EpCAM)—which induced aggressive unintentional intestinal inflammation in patients, preventing escalation to therapeutic doses and ultimately leading to program termination34. Given the rapid onset of side effects, IELs localized within the basolateral epithelial junctions have been hypothesized to elicit this damage. To assess whether IEL-containing IIOs could have predicted targeting of the heatlthy epithelium we treated IIOs with an EpCAM-targeting, T cell-bispecific (TCB) molecule at clinically relevant concentrations34. Unlike organoids cultured with PBMCs, IIOs were targeted in a TCB dose-dependent manner at concentrations as low as 40 pg ml−1 and as early as 8 h following treatment, as demonstrated by the detection of caspase 3/7 (Fig. 3a–c and Extended Data Fig. 3a). We assessed T cell behaviour at early (5 h), middle (24 h) and late (48 h) time points by digestion and staining of IIOs for surface and intracellular markers of T cell activation and cytotoxicity (Extended Data Fig. 3b). Identification of effector populations, based on the expression of TNF, IFNγ and granzyme B (Gzmb), showed that over 90% of responding cells were intestinal TRM cells (Extended Data Fig. 3c). Furthermore, we observed that TRM cells began expressing CD38, HLA-DR and Ki67—activation and proliferation markers found on cells that drive colitis-like pathology in vivo35,36 (Extended Data Fig. 3d). Supernatant analysis reinforced these findings, with key cytokines TNF, IFNγ, Gzmb, IL-2 and GM-CSF all more detectable in TRM cell IIO cultures (Extended Data Fig. 3e)
a, Representative images examining induction of green caspase 3/7 signal within IIO co-cultures 24 h following supplementation with EpCAM TCB. b, Quantification of caspase 3/7 signal from a. Two-way analysis of variance (ANOVA) with Sidak’s multiple-comparisons test. Triplicate IIO cultures representative of experiments performed with three independent biological donors. MFI, mean fluorescence intensity. c, Quantification of caspase 3/7 signal in IIO co-cultures treated for 72 h across a range of EpCAM TCB. Two independent IIO cultures were run across a six-dose titration of EpCAM TCB; mean (black line) and s.d. (grey shading). d, Single-cell transcriptomic profiles of gut-derived immune cells from IIO model were integrated and grouped into 14 distinct cell states as represented by colours in the UMAP embedding. Organoid schematic adapted with permission from ref. 60. e, Dotplot summarizing the expression patterns of representative genes across the clusters identified in d. f, Integrated UMAP embedding (left) and proportional distribution (right) of gut-derived immune cells from IIO model, coloured by treatment and profiling time. g, Barplot showing significantly enriched Gene Ontology biological processes for activated cell states (top) and heatmap showing average expression profiles of corresponding associated genes (bottom). h, Dotplot summarizing the expression pattern of representative genes involved in proliferation, signalling and cytotoxicity in activated T cell populations, as captured by scRNA-seq snapshots at different time points and under various treatment conditions. i, Flow cytometry plots visualizing expression of TNF, IFNγ, Gzmb and Ki67 across different time points within CD4+ and CD8+ TRM cells isolated from IIO cultures. Representative of five biologically independent experiments. Scale bars, 1 mm.
We found that, although circulating memory cells were more potent than their naive counterparts, responses were significantly weaker than those of matched TRM cells (Extended Data Fig. 3f). Within TRM cells, CD103− (lamina propria) and CD103+ (intraepithelial) fractions triggered equivalent epithelial targeting (Extended Data Fig. 3g,h), which was also maintained in longer-term IIO cultures with TCB added 10 days following co-culture (Extended Data Fig. 3i).
To investigate whether TRM cell-mediated inflammatory responses might affect circulating immune cells, we supplemented IIOs with PBMCs and treated them with TCB (Extended Data Fig. 4a). The presence of TRM cells led to enhanced PBMC integration within organoids compared with organoid + PBMCs cultured in the absence of TRM cell (Extended Data Fig. 4b). Furthermore, we noted synergistic induction of myeloid-associated cytokines IL-1β, IL-6 and IL-8 in IIO cultures supplemented with PBMCs, suggesting that a tissue-resident immune compartment can potentiate responses of peripheral immune cells within the system (Extended Data Fig. 4b–d).
The mechanisms of severe rapid-onset toxicities caused by T cell-targeted therapies are unclear, because patients who experience them are not biopsied in the acute phase. IIOs, which we show can recapitulate aspects of clinical outcomes, provide the opportunity for in-depth analysis of the underlying immunological events. We used scRNA-seq to interrogate the transcriptomic dynamics underlying TCB-dependent TRM cell activity at the onset (4 h) and peak (48 h) of epithelial cell targeting. Lymphocyte populations within the integrated dataset were annotated using differential gene expression, together with previously published signatures and surface markers35,37,38,39,40, showing predominantly T cell, but also macrophage and B cell, populations in this particular specimen (Fig. 3d,e and Supplementary Tables 1 and 2). TCB treatment induced a shift in proportions of both T and B cell states relative to the non-targeting control (Fig. 3f), driven by the emergence of several effector populations at both time points. Particularly prominent at 4 h was a CD4+ T helper 1-like (TH1) population (c9) (Fig. 3d–f), characterized by rapid induction of TNF and IFNG signalling that became downmodulated over time (Fig. 3g,h). At 48 h we observed the emergence of an activated CD8+ IEL population (c5) (Fig. 3d–f) expressing genes related to cytotoxicity (such as GZMB), TCR signalling and T cell migration (Fig. 3g,h). Concurrently, a population of cycling (MKI67-expressing) CD4+ T cells (c12) and an activated population of B cells (c11) appeared whereas the regulatory T cell population (c1) diminished (Fig. 3d–g). Importantly, key gene expression changes detected by scRNA-seq were mirrored by changes at the protein level, as recorded by flow cytometry (Fig. 3i). We observed early induction of TNF in CD4+ cells at 4 h (corresponding to c9) whereas cytotoxic Gzmb was upregulated at 48 h, in line with the appearance of c5. Likewise, Ki67 was strongly upregulated within a subset of CD4+ cells at 48 h, co-occurring with the appearance of c12 within the scRNA-seq analysis. IIO cell heterogeneity dynamics bear striking similarities to those observed within primary samples from patients experiencing spontaneous and immune checkpoint inhibitor (ICI)-induced intestinal inflammation. For example, the emergence of a cytotoxic CD8+ T cell population was one of the main features of patients experiencing ICI-induced colitis35,36. Likewise, regulatory T cell transition towards pathogenic cytokine production and the appearance of activated IFNγ-responsive B cell populations are well-described features of colitis pathophysiology41,42,43. These similarities suggest that IIOs may be used to recapitulate and study intestinal inflammation in a tractable in vitro setting.
Next we charted the activation dynamics of the clinically relevant CD8+ T cell population. Using Diffusion Maps44, we computed a pseudotemporal ordering of populations c3 and c5 (Fig. 4a and Extended Data Fig. 5a) and observed a marked correlation with experimental time (Fig. 4b and Extended Data Fig. 5b). This reconstructed activation trajectory allowed for characterization of the transcriptional dynamics underlying CD8+ TRM cell activation (Extended Data Fig. 5c). We observed induction of glycolytic regulators ENO1 and HIF1A and suppression of TCF7 and ZBTB32 (refs. 45,46,47,48) that probably facilitate the appropriate metabolic profile for full activation. Simultaneously CCL5, important for immune cell recruitment49, and IL7R, which mediates cytotoxic activation50, correlated with the induction of cytotoxicity genes GZMA, GZMB and NKG7. Sequencing of biopsies of patients suffering from drug-induced colitis demonstrated the presence of both cytotoxic (CTL) and IEL CD8+ T cell populations35. By cross-referencing TCB-treated IIOs to this dataset, we found that IIO CD8+ T cells acquired gene signatures related to a cytotoxic and IEL state, mirroring those observed in clinical samples (Extended Data Fig. 5d,e). The concurrent increase in lymphocyte–epithelium association and cytotoxicity may underlie the severe clinical adverse events triggered by these molecules.
a, CD8+ T cell activation in the IIO model was analysed using diffusion maps. Plot represents CD8+ T cells on the first two diffusion components (DCs), coloured by cell state. b, Density plot showing distribution of CD8+ T cells along the reconstructed pseudotime (x axis) grouped by treatment condition and time point. c,d, Barplot showing differentially expressed genes for CD4+ cytotoxic TH1 population (c9) (c) and T-bet+ effector B cells (c11) (d) at 4 h (light grey) and 48 h (dark grey) following EpCAM TCB treatment. e, Ligand–receptor pairing analysis of IIO immune cell populations. Ligands and receptors are coloured based on coexpression module, with some representative genes labelled. Heatmap (right) shows the average expression of each gene within a module across each cell cluster. f, Circle plots illustrate signalling interactions received by T-bet+ effector B cells (c11) (left) and CD8+ Act. IELs (c5) (right). g, In silico perturbation analysis simulating the loss (KO) of TNF in the IIO model treated with EpCAM TCB. Plots show predicted perturbation-induced state transition (top) and enrichment (bottom). h, Schematic of experiments performed to inhibit TNF signalling and cell migration. Organoid cartoon adapted with permission from ref. 60. i, Representative images showing induction of caspase 3/7 signal within IIO co-cultures 40 h following supplementation with either a non-targeting control TCB or 5 ng ml−1 EpCAM TCB, with or without 10 µM ROCKi or a TNF blocking antibody. j, Bi-hourly quantification of caspase 3/7 signal in IIO cultures; top, comparison of EpCAM TCB + isotype with EpCAM TCB + adalimumab; bottom, comparison of EpCAM TCB + vehicle with EpCAM TCB + ROCKi. Data represent mean and standard deviation (grey shading) of three independent biological replicates; two-tailed unpaired t-test of the area under the curve generated for each condition. Scale bar, 200 µm. FC, fold change.
Aside from CD8+ CTLs, other populations showed clinically relevant dynamics of early- versus late-transcriptional hallmarks. Consistent with clinical reports51, a TH1 population (c9) shifted from a cytokine-producing to a cytotoxic state (Fig. 4c and Extended Data Fig. 5f). Likewise, the early IFN-responsive B cell population (c11) showed a transcriptional landscape distinct from that of the late, activated state (Fig. 4d and Extended Data Fig. 5g). We performed a receptor–ligand pairing analysis and investigated how activated phenotypes might emerge via intercellular signalling (Fig. 4e). Our model implicates TH1 cells (c9) as a major organizational hub, instructing B cells (c11) and CD8+ T cells (c5) through the secretion of numerous signalling factors (Fig. 4f and Extended Data Fig. 5h,i). Meanwhile, the CD4+ T cell (c9) expression of TNF corresponded to increased CALM1 expression in activated CD8+ IELs (c5), potentially augmenting TCR-induced calcium signalling and T cell maturation52 (Fig. 4f and Extended Data Fig. 5j). Ligand-to-target signalling models and network propagation analysis53 reaffirmed these observations, suggesting that TH1-produced IFNG may mediate B cell activation whereas TNF and IL2 act in tandem to orchestrate cytotoxic CD8+ cell maturation (Extended Data Fig. 5h,i).
A key advantage of human model systems is their amenability to experimental manipulation as a means of defining the roles of putative regulators. Given the effectiveness of TNF-blocking antibodies in the treatment of autoimmune disease, and the early induction of TNF in our model (Fig. 3h,i), we investigated its role in the promotion of differentiation and activation. In silico perturbation analysis predicted that the complete removal of TNF from IIOs would prevent TRM cell activation (Fig. 4g). Antibody neutralization of TNF using adalimumab in IIOs following TCB treatment confirmed this forecast, significantly reducing the TRM cell expression of key activation and differentiation markers ICAM-1, 4-1BB, CD25 and Gzmb (Extended Data Fig. 6a). Furthermore, expression of CCL2, a chemokine expressed by the epithelium in response to inflammation54, as well as epithelial cell apoptosis itself, were significantly reduced (Fig. 4h–j and Extended Data Fig. 6b). This effect appeared to be mediated by TNF effects on TRM cells rather than direct cytotoxicity to the epithelium, because direct organoid exposure to recombinant TNF had no impact on epithelial caspase signal (Extended Data Fig. 6c,d). Having confirmed the impact of neutralization of a clinically validated pathway, we used the transcriptomic data defining TRM cell identity (Fig. 2) to suggest new factors that could be manipulated to quell inflammation. Given the rapid TRM cell locomotion within the ECM, we hypothesized that T cell motility might, in part, be responsible for the outcome. To test this hypothesis we used the ROCK1/2 inhibitor (ROCKi) Y-27632 to abrogate cell motility within TCB-treated IIOs. Strikingly, we found that ROCK inhibition reduced epithelial cell apoptosis even more efficiently than TNF blockade (Fig. 4h–j), simultaneously suppressing TRM cell motility (Supplementary Video 4 and Extended Data Fig. 6e) and the induction of T cell activation markers and cytolytic molecules such as perforin and Gzmb (Extended Data Fig. 6f). Intracellular staining showed that TNF induction was also suppressed but overall T cell viability was unchanged (Extended Data Fig. 6g,h). In addition to TNF- and Rho-mediated signalling, the transcriptomic and ligand–receptor analyses shown provide a wide range of putative targets for managing inflammation, which are worth investigation in future studies.
Immune checkpoint inhibitors such as anti-CTLA-4 and anti-PD-1 induce durable responses in a wide range of human cancers but simultaneously trigger enterocolitic symptoms in 10–15% of patients by exacerbation of contemporaneous immune responses to gut microflora or viral infections35,55,56,57. Because CD8+ TRM cells have been described as key drivers of this pathology, we tested whether our IIO cultures could recapitulate this phenomenon. We pretreated TRM cell or matched PBMC controls with allogeneic dendritic cells to recapitulate the role on antigen-presenting cells, either in the presence or absence of anti-CTLA-4 (ipilimumab) and anti-PD-1 (nivolumab), and then exposed them to organoids. Although we did not detect changes in caspase signal (Extended Data Fig. 7a), the more sensitive lactate dehydrogenase (LDH) assay showed a statistically significant increase in signal in one of the two TRM cell biological replicates treated with ICIs (Extended Data Fig. 7b). The response was associated with an increase in CD8+ TRM cells expressing CD38, Gzmb and Ki67 (Extended Data Fig. 7c,d), similar to the disease-driving populations described in patients35,36. Interestingly, both PBMCs and TRM cells from either donor showed augmented activation following ICI treatment (Extended Data Fig. 7e), but the increase in LDH signal was present only under the TRM cell condition in which pathogenic CD8+ T cell numbers were increased (Extended Data Fig. 7c,d), suggesting that close association with the epithelium might be required for damage.
We thus describe human IIOs, comprising human intestinal epithelium and autologous TRM cells, a subpopulation of which is directly integrated within the IIO epithelial barrier, as orchestrated by TRM cell-enriched transcriptomic programs governing cell motility and adhesion. Crucially, IIOs formed following co-culture with TRM cells but not blood-derived lymphocytes, which failed to migrate and interact with organoids. Although epithelial cell identity did not shift markedly following TRM cell integration, we hypothesize that TRM cells influence epithelial barrier integrity, defence and regeneration5,6. Given that these shifts probably manifest under conditions of challenge, adaptations to the model to allow luminal access and routine introduction of gut pathogens is an exciting area we are actively pursuing.
The inclusion of a tissue-appropriate immune compartment extends the utility of organoids beyond epithelium-centred questions. We use IIOs to recapitulate intestinal inflammation caused by TCBs. IIOs treated with TCBs undergo rapid apoptosis, consistent with early-onset diarrhoea and epithelial lesions in patients34. Importantly, whereas co-cultures with PBMCs have been shown to capture similar outcomes, effects occur at concentrations 1,000-fold higher than clinical doses and, even then, with a delayed onset58. Through dissection of the transcriptomic changes induced by TCB treatment, we show that adverse outcomes are underpinned by dynamic interlineage immune interactions that parallel mechanisms associated with checkpoint inhibitor-induced intestinal inflammation and inflammatory bowel disease35,36,51.
Our model provides the advantages of direct perturbation and hypothesis testing. Whereas multiomic analyses of primary patient samples provide rich catalogues of differences between normal and diseased states, the exact roles of differentially regulated parameters are difficult to ascertain. Having demonstrated that IIOs respond to clinically effective mitigation strategies, we use them to identify new approaches for the management of TCB-mediated toxicities. We find that blocking of TRM cell motility through the Rho pathway helps dampen inflammation. Intriguingly, small molecules that target this pathway are being developed as fibrosis inhibitors for inflammatory bowel disease59. Bearing in mind that our simple model recapitulates both the phenotypic outcomes and cellular interactions that mediate them, IIOs can help investigate tissue-resident immune responses in contexts far beyond drug-induced inflammation, including tumorigenesis and infectious and autoimmune diseases.
Methods
Human samples
Human intestinal tissue samples and annotated data were obtained, and experimental procedures performed, within the framework of the non-profit foundation HTCR (Munich, Germany), including informed patient consent. The framework of the HTCR Foundation has been approved by the ethics commission of the Faculty of Medicine in the Ludwig Maximilian University (no. 025-12) and the Bavarian State Medical Association (no. 11142). Twenty-seven consenting patients underwent visceral surgery with partial resection of intestine (16 males, 11 females, age range 40–89 years; precise intestinal regions are listed in Supplementary Table 4) for various oncologic indications, including pancreaticojejunostomy due to pancreatic adenocarcinoma (example no. ICD10, codes C18.x, C24.x and C25.x). We used micro- and/or macroscopically tumour-free regions of resectates for further preparation.
Isolation of TRM cells and donor-matched crypts for organoid generation
Intestinal tissue was first processed by removal of the underlying muscularis, serosa and fat from the basal side of the tissue by pinching with forceps and trimming away with scissors. The remaining mucosal tissue was washed with PBS (supplemented with penicillin (1,500 U ml−1), streptomycin (1,500 μg ml−1) and gentamicin (500 μg ml−1)) multiple times before using a scalpel to remove excess mucus from the luminal side and blood vessels from the basal side. Trimmed, cleaned tissue was then cut into square explants (approximately 5 × 5 mm3) with a scalpel. Lymphoid follicles such as Peyer’s patches were not separated from lamina propria tissue. Two methodologies were used to isolate intestinal TRM cells. For the scaffold-based egression protocol (adapted from ref. 20), each explant was loaded onto a 9 × 9 × 1.5 mm3 tantalum-coated, carbon-based scaffold (Ultramet) and cultured in 24-well plates containing 1 ml of cytokine-free cell culture medium (RPMI 1640, 10% fetal calf serum (FCS), penicillin (1,500 U ml−1), streptomycin (1,500 μg ml−1), gentamicin (500 μg ml−1) and amphotericin (12.5 μg ml−1)). Twenty-four hours later, scaffolds and tissue were removed and egressed cells were harvested from the bottom of the culture well via pipetting. For the enzymatic digestion protocol, explants were digested using the Human Tumour Dissociation Kit with the gentleMACS Octo Dissociator program 37C_h_TDK_1 (Miltenyi Biotec), as per the manufacturer’s instructions, reducing Enzyme R content by 80% to minimize surface antigen cleavage. Cells were then counted, phenotyped by flow cytometry and frozen before use in downstream applications. For isolation of donor-matched crypts for organoid generation, a lamelle was scraped over a 3 cm2 piece of trimmed tissue to remove intestinal protrusions/villi. Tissue was then incubated in ice-cold PBS + 10 mM EDTA with vigorous shaking for 30 min to break down epithelial cell junctions. Tissue was retrieved and a lamelle used to scrape off crypts into DMEM-F12 1% bovine serum albumin (BSA) collection buffer. Crypts were centrifuged, resuspended in Matrigel Matrix GFR (Corning) and cultured in IntestiCult Organoid Growth Medium with 10 μM Y-27632 (STEMCELL Technologies). Absolute authentication was confirmed by expression of cell lineage-defining markers and transcriptomic analysis. All lines used in the studies were verified as negative for mycoplasma before further experimentation.
Preparation and culture of IIOs, including treatment
In-passage organoids, approximately 2 weeks to 1 month following initial isolation, were cultured for 4 days post split in IntestiCult Organoid Growth Medium and then switched to IntestiCult Organoid Differentiation Medium (STEMCELL Technologies) for 72 h to promote epithelial stem cell differentiation. On the day of co-culture set-up, medium was aspirated from the well and organoids were washed with PBS and treated with ice-cold Cell Recovery Solution (Corning) for 40 min. The solution was collected and centrifuged to harvest the liberated organoids. Donor-matched TRM cells or PBMCs were thawed, combined with the liberated organoids and resuspended in either Matrigel Matrix GFR (Corning) or, if co-cultures were to be formalin fixed, a 50:50 mixture of Matrigel Matrix GF and 4 mg ml−1 Cultrex Rat Collagen I (R&D Systems). For time-lapse live-imaging experiments, immune cells were labelled with CellTrace Far Red or CFSE (ThermoFisher) before mixing with organoids. Organoids were used at a concentration double their standard passaging density, whereby a 20 µl dome was harvested and resuspended in 10 µl of matrix, whereas immune cells were used at a density of 15,000 mm−3 of resuspension volume. A 50:50 ratio of RPMI 1640 10% FCS and IntestiCult Organoid Differentiation Medium was used for culture. For assessment of TCB-based cytotoxicity, an EpCAM-CD3 bispecific antibody, or its associated non-targeting control (which contains a CD3 binder on one arm and a non-specific DP47 arm), was supplemented into the culture medium following co-culture set-up, or 10 days following co-culture set-up in the case of experiments with longer-term IIO cultures, typically at a concentration of 5 ng ml−1. When used, blocking antibodies to TNF (adalimumab, InvivoGen) were added at a concentration of 1 µg ml−1. To investigate the role of ROCK signalling on TRM cell activation, 10 µM Y-27632 was added daily for the duration of the co-culture. For longer-term co-cultures (up to 1 month), a 50:50 ratio of RPMI 1640 10% FCS and IntestiCult Organoid Growth Medium, supplemented with IL-2 (10 IU ml−1) (Roche) and IL-15 (2 ng ml−1; BioLegend) was used. Medium was changed three times per week and the culture split once per week. Cultures were supplemented with 10 µM Y-27632 (STEMCELL Technologies) following splitting. For investigation of TRM cell-generated inflammation to study recruitment of circulating T cells into the epithelium, CellTracker CMFDA Green (ThermoFisher) was used to stain PBMCs at a final working concentration of 1 µM. For single TRM cell–organoid and PBMC–organoid co-cultures, the density of immune cells previously described was used. However, for TRM cell–PBMC–organoid co-culture, both TRM cell and PBMC compartments were seeded at 15,000 cells mm−3 of resuspension volume.
Flow cytometry analysis of immune-organoids
Triplicate wells of immuno-organoid co-cultures from each condition were harvested 5, 24 and 48 h post treatment. At 4 h preceding culture harvest at each time point, wells were treated with Protein Transport Inhibitor Cocktail (ThermoFisher) to facilitate intracellular accumulation of cytokine protein. Co-cultures were washed with PBS and then digested to single cells using Accutase solution (STEMCELL Technologies) at room temperature for approximately 30 min. Cell suspensions were passed through a 70 μm strainer and stained for surface proteins (Supplementary Table 3). Cells were then fixed and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (ThermoFisher), and subsequently stained for intracellular and intranuclear proteins. Stained cell suspensions were acquired on a BD Fortessa X-20 using BD FACSDiva Software v.9.7 and analysed using FlowJo v.10. Example gating strategies are shown within the source data of the relevant figures in Supplementary Information. To facilitate visual representation across one plot, samples from different time points and treatments were concatenated and separated along the y axis.
Luminex supernatant analysis of immune-organoids
Following sampling, supernatants were immediately stored at −80 °C until measurement. For measurement of cytokines (Gzmb, IFNγ, IL-2, IL-4, IL-6, IL-10, TNF, MCP-1, IP-10 and GM-CSF) the customized Invitrogen ProcartaPlex multiplex immunoassay (reference no. PPX-10-MXFVMZC) was applied and used according to the manufacturer’s instructions. In short, capture beads were added to a 96-well flat-bottom plate, washed with an automatic plate washer (405TS microplate washer, Bioteck) and incubated with either the diluted supernatants or provided standards for 2 h at room temperature. Next the beads were washed and detection antibodies added to the plate for 30 min at room temperature, followed by a further wash step. The beads were then incubated with Streptavidin-PE for a further 30 min at room temperature before final washes. Lastly the beads were resuspended in the acquisition buffer and the plate was read on a Bio-Plex-200 instrument (Bio-Rad) using the corresponding Bio-Plex Manager Software v.6.2.
Isolation of T cell subsets for organoid co-culture
For organoid co-culture with specific subsets of immune cells, magnetic-activated cell separation MS columns and MicroBeads (Miltenyi Biotec) were used for subset enrichment following the manufacturer’s instructions. To enrich memory T cells from PBMCs, CD45RO MicroBeads were used (Miltenyi Biotec). To enrich for CD103+/− cells from TRM cells, cells were first labelled with biotin anti-human CD103 antibody (BioLegend) and subsequently purified using anti-biotin MicroBeads (Miltenyi Biotec). Purity of the enriched cells was validated by flow cytometry.
Caspase 3/7-based epithelial cell cytotoxicity assay
Intestinal immuno-organoid cultures were prepared in 4 µl of Matrigel Matrix GF per well of a PhenoPlate 96-well microplate (Revvity), with cell ratios and media as described above. Apoptosis was assessed using the CellEvent Caspase 3/7 Detection Reagent (Invitrogen), either during TCB treatment or at specific intervals. CellEvent Caspase 3/7 Detection Reagent was added to culture medium at 1:1,000. Samples were imaged in confocal mode at ×5 magnification (air objective) with an Operetta CLS (Perkin Elmer) covering approximately a 450 µm z-stack, starting at −150 µm. Distance between z-stacks was set to the minimum of 27 µm for the ×5 objective (autofocus, two-peak; binning, 2). Channels selected were bright-field and predefined AlexaFluor 488. Per well, five fields were acquired, covering nearly the entire surface of the 96-well PhenoPlate plate; CO2 was set to 5% and temperature to 37 °C. Caspase 3/7 fluorescence signal intensity was quantified using Opera Harmony software v.4.9 (PerkinElmer). Briefly, segmentation of organoids was done using ‘Find Texture Regions’ based on the bright-field signal only, followed by ‘Select Region’ and ‘Find Image Region’ to segment single organoids as objects. Next, ‘Calculate Morphology Parameters’ was performed to select objects above 10,000 µm2 with ‘Select Population’. Next, Caspase 3/7 fluorescence signal per individual organoid was determined using ‘Calculate Intensity Properties’ of the AF 488 channel within these objects.
Time-lapse imaging of IIOs
Before IIO or organoid + PBMC-derived T cell co-culture, matched TRM cells and PBMCs were thawed and apoptotic cells removed using the Dead Cell Removal Kit (Miltenyi Biotec). T cells were isolated from PBMCs using the EasySep Human T Cell Isolation Kit (no. 17951, STEMCELL Technologies) and the EasySep Magnet (no. 18000, STEMCELL Technologies), following the manufacturer’s instructions. Immune cells were labelled with CellTrace Far Red (no. C34564, ThermoFisher) and IIO or PBMC + organoid co-cultures were prepared as described in ‘Preparation and culture of intestinal immune-organoids, including treatment’. Time-lapse live imaging was performed 16 h post co-culture set-up with a Leica STELLARIS 8 confocal microscope using a water-immersion objective (HC FLUOTAR L VISIR ×25/0.95 numerical aperture WATER) and 0.85 zoom. Images were obtained in bidirectional mode with 1,024 × 1,024 pixels at 600 Hz. Images were acquired every 38 s with 84 µm z-stacks (z-steps, 4 µm). Samples were imaged between 42 and 60 min, where indicated. During imaging, samples were maintained in an incubation chamber (The Box, Life Imaging Services) at 37 °C and 5% CO2. Following acquisition, maximum-intensity projections were generated with Leica Las X software and later exported as AVI files using ImageJ v.1.54i. Cell videos were analysed using CellProfiler v.4.2.5. The number of frames per condition was equalized for direct comparison. Briefly, cells were segmented at each frame with Multi-Otsu, morphological parameters were extracted and segmented cells were tracked over time using an overlap of two pixels. The output per cell was analysed using KNIME v.5.2.4. The means and standard deviation of morphological features per track over time were calculated. The standard deviation of morphological features over time is a measure of the dynamism of cells because they use amoeboid motility. In addition, track lifetime and track total distance were calculated. Tracks with fewer than 20 frames were discarded.
Monocyte-derived dendritic cell generation for ICI experiments
Monocyte-derived dendritic cells were generated using the Mo-DC generation Toolbox (Miltenyi Biotec) following the manufacturer’s instructions. Briefly, monocytes were isolated from PBMCs by magnetic separation over magnetic-activated cell separation columns with CD14 MicroBeads. Monocytes were cultured in standard tissue culture flasks in the provided Mo-DC Differentiation Medium for 7 days, with renewal of the medium every 2–3 days. Maturation of dendritic cells was initiated at day 7 for 3 days by the addition of 6,000 IU ml−1 human TNF (Miltenyi Biotec). One day before usage, 100 ng ml−1 lipopolysaccharide (Sigma-Aldrich) was added to activate dendritic cells overnight. Cells were harvested by incubation in 2× EDTA in PBS for 10 min at 37 °C. Dendritic cells were counted and plated at 40,000 per well of an ultralow-attachment, round-bottom, 96-well plate (Corning). TRM cells and PBMCs from the same donors were thawed, counted and combined with dendritic cells at 120,000 cells per well. Either immune checkpoint inhibitors (nivolumab and ipilimumab, both Bristol-Myers Squibb) or isotype controls were added at 20 µg ml−1, in RPMI 1640 with 10% FCS. Dendritic/T cell co-cultures with or without ICIs were cultured for 4 days before combining immune cells with matched organoids. Organoids were harvested as described in the preparation and culture of IIO before being mixed with the corresponding cells from the mixed lymphocyte reaction. IIO cultures were plated in 5 µl of Matrigel Matrix GFR domes and maintained in 50:50 RPMI 1640 with 10% FCS (ThermoFisher) and IntestiCult Organoid Growth Medium (STEMCELL Technologies) for 48 h, with or without ICIs. Cell death was monitored by live imaging with Caspase 3/7, and LDH in the supernatant was measured every 24 h.
LDH assay
Supernatants of the triculture were used directly following sampling for measurement. A cytotoxicity detection kit (Roche) was used according to the manufacturer’s instructions. Briefly, the standard curve was prepared and supernatants were diluted in PBS and incubated with the reaction mix for 30 min at room temperature in the dark. Following incubation the plate was read using a PerkinElmer Envision 2104 Multilable reader with absorbance at 490 nm.
Recombinant TNF treatment of organoid cultures
Four days following passage, organoids were harvested with cell recovery solution (Corning) and seeded onto a PhenoPlate 96-well microplate (Revvity) in 5 µl of Matrigel Matrix GFR droplets. Organoids were treated for 72 h with human recombinant TNF (Miltenyi Biotec; highest dose, 156 ng ml−1, seven-dose titration, fivefold dilution). TNF-induced apoptosis was monitored by live imaging every 2 h on an Operetta using the cell event Caspase 3/7 detection reagent as described above.
FFPE embedding of co-cultures
To formalin-fixed, paraffin-embedded (FFPE) co-cultures the samples were seeded in a 50% (v/v) Matrigel–Collagen I matrix. Wells were washed once with 1× DPBS before fixation with 4% paraformaldehyde (PFA) in the 24-well Clear TC-treated plate. Following 30 min of fixation at room temperature, the wells were washed three more times before complete aspiration of 1× DPBS; 400 µl of preliquefied HistoGel (ThermoScientific) was then dispensed into 24-well Clear TC-treated plates. Following polymerization of HistoGel (10 min at 4 °C), the organoid–HistoGel ‘platelet’ was carefully lifted out of the 24-well Clear TC-treated plate using a thin metallic spatula. Samples were then distributed into biopsy cassettes and dehydrated overnight using a Vacuum filter processor (Sakura, TissueTek VIP5). The following day, samples were embedded in liquid paraffin.
Microtome sectioning
FFPE blocks were, in general, sectioned at a thickness of 5 µm and transferred on Superfrost Plus Adhesion microscope slides (Epredia). Where indicated, thickness differs. Slides were incubated in a slide oven overnight at 37 °C.
FFPE-based mIF
Multiplex immunofluorescence (mIF) staining of FFPE slides was performed using a Ventana Discovery Ultra automated tissue stainer (Roche Tissue Diagnostics). Slides were first baked at 60 °C for 8 min and subsequently further heated to 69 °C for 8 min for subsequent deparaffinization. This cycle was repeated twice. Heat-induced antigen retrieval was performed with Tris-EDTA buffer pH 7.8 (Ventana) at 92 °C for 32 min. After each blocking step with Discovery Inhibitor (Ventana) for 16 min, the Discovery Inhibitor was neutralized. Primary antibodies were diluted in Discovery Ab diluent (Ventana). Primaries were detected using appropriate anti-species secondary antibodies conjugated to horseradish peroxidase (HRP, OmniMap Ventana; Supplementary Table 3). Subsequently, the relevant Opal dye (Akoya Biosciences) was applied. Following every application of a primary, respective secondary antibody and Opal dye, an antibody neutralization and HRP-denaturation step was applied to remove residual antibodies and HRP before starting the staining cycle again with the Discovery Inhibitor blocking step. Lastly, samples were counterstained with DAPI (Roche).
mIF staining using Opal dyes from Akoya was digitized with multispectral imaging by Vectra Polari (PerkinElmer) using MOTiF technology at ×20 magnification for all seven colours (Opal 480, Opal 520, Opal 570, Opal 620, Opal 690, Opal 780 and DAPI). Slides were scanned in a batch manner to ensure identical imaging settings and cross-comparability for subsequent image analysis with HALO AI. Next, unmixing of channels and tiling of images was performed with PhenoChart (v.1.0.12) and inForm (v.2.4). Tiles were fused in HALO (Indica labs, v.3.2.1851.328).
High-resolution mIF was obtained using a STELLARIS 8 microscope (Leica) with a ×40/1.1 numerical aperture water-immersion objective (HC FLUOTAR L VISIR ×25/0.95 numerical aperture WATER) and 1.0 zoom. A white-light laser (440–790 nm) facilitated imaging of all Opal dyes mentioned above, and channels were acquired sequentially to reduce cross-talk. Images were obtained in bidirectional mode with 2,048 × 2,048 pixels (pixel size, 273.8 × 273.83 nm2) at 600 Hz. Where indicated, images were acquired with z-stacks of 10–15 µm (z-steps, 1 µm), three-dimensionally reconstructed and shown in ‘Maximum’ mode using Leica Application Suite X software (Leica).
Image analysis by FFPE-based mIF
Image analysis of mIF images was performed with HALO AI (Indica Labs, v.3.2.1851.328). Briefly, single organoids were automatically detected using a deep learning algorithm trained to distinguish matrix and organoids (iterations, 5,000; cross-entropy, 0.32; DenseNet AI V2 Plugin). Following rapid validation, organoids were annotated as individual regions of interest, objects. Only objects over 7,500 µm2 were considered positive.
The HighPlex FL v.4.2.14 module was used to perform nuclear segmentation based on DAPI+ cells (assisted by HALO’s integrated AI-default ‘nuclear segmentation type’) and specific marker identification. For quantification, DAPI+ nuclei and markers for each distinct cell type of interest were merged (taking membranous and nuclear signals into account). Either secondary-only negative controls on the tissue of origin or organoid samples embedded within the same block served, and were then exposed to the full antibody-staining panel as a negative control sample to set the threshold for prevention of biased adjustments. The HighPlex FL analysis module was deployed on previously generated regions of interest of the organoids using integration of the classifier in the module. The number of T cells integrated/infiltrated into the organoid was normalized according to either the number of epithelial cells (non-TCB-treated organoids) or organoid area (EpCAM-targeted organoids). Surface markers of distinct T cells were used to phenotype these accordingly. Data was exported from HALO and analysed in GraphPad prism.
Single-cell dissociation of IIOs
Intestinal immuno-organoids were dissociated as described previously62. In short, organoids were dislodged, mechanically dismantled and transferred to 1% BSA-coated tubes. Organoid fragments were centrifuged at 400g for 4 min at room temperature. The supernatant was removed and enzymes of the neural tissue dissociation kit (Miltenyi Biotec) were mixed in HBSS/1%BSA buffer. Organoid fragments were then dissociated to single cells for a total of 30 min with thorough pipetting every 7 min. Next, cells were filtered through a 40 μm filter, with single cells centrifuged at 450g for 4 min at room temperature and subsequently resuspended in DPBS 1% BSA. Single-cell libraries were prepared on the 10X Genomics platform using the Chromium Next GEM Single Cell 3′ Kit v.3.1.
scRNA-seq data preprocessing
CellRanger (v.6.0.2, 10X Genomics) was used to extract unique molecular identifiers, cell barcodes and genomic reads from the sequencing results of 10X Chromium experiments. Next, count matrices, including both protein coding and non-coding transcripts, were constructed aligning against the annotated human reference genome (GRCh38, v.3.0.0, 10X Genomics). For removal of potentially damaged or unhealthy cells and improvement in data quality, the following filtering steps were performed in addition to the built-in CellRanger filtering pipeline. Cells associated with over 50,000 transcripts—usually less than 1% of the total number of samples—were removed; and cells associated with a low number of unique transcripts—fewer than 500 unique transcripts detected (1% of the total number of samples)—were removed. Cells with over 20% of mitochondrial transcripts were removed. Transcripts mapping to ribosomal protein coding genes, as well as to mitochondrial genes, were removed, together with transcripts detected in fewer than ten samples.
Normalization with SCTransform
For normalization and variance stabilization of the molecular count data of each scRNA-seq experiment we used the modelling framework of SCTransform in Seurat v.3 (ref. 63). In brief, a model of technical noise in scRNA-seq data is computed using ‘generalized gamma poisson regression’64. The residuals for this model are normalized values that indicate divergence from the expected number of observed unique molecular identifiers (UMIs) for a gene in a cell, given that gene’s average expression in the population and cellular sequencing depth. In addition, a curated list of cell cycle-associated genes, available within Seurat, was used to estimate the contribution of cell cycle and remove this source of biological variation from each dataset to increase the signal deriving from more interesting processes. The residuals for the top 2,000 variable genes were used directly as input for computation of the top 100 principal components by principal component analysis (PCA) dimensionality reduction through the RunPCA() function in Seurat. Corrected UMIs, which are converted from Pearson residuals and represent expected counts if all cells are sequenced at the same depth, were log transformed and used for visualization and differential expression analysis.
Both primary intestinal biopsy samples and primary multiorgan biopsy samples were processed as described above. However, these did not undergo any cell filtering because quality control steps had already been performed in the respective published studies.
Doublet removal with DoubletFinder
For each scRNA-seq experiment, DoubletFinder65 (v.2.3.0) was used to predict doublets in the sequencing data. In brief, this tool generates artificial doublets from existing scRNA-seq data by merging randomly selected cells, which are then preprocessed together with real data and jointly embedded on a PCA space that serves as a basis for finding each cell’s proportion of artificial k-nearest neighbours. For this step we restricted the dimension space to the top 50 principal components. Finally, the proportions of artificial k-nearest neighbours values were rank ordered according to the expected number of doublets, and optimal cut-off selected through receiving operating characteristic analysis across pN–pK parameter sweeps for each scRNA-seq dataset: pN describes the proportion of generated artificial doublets, with pK defining principal component neighbourhood size. To achieve maximal doublet prediction accuracy the mean variance-normalized bimodality coefficient was leveraged. This provides a ground-truth-agnostic metric that coincides with pK values that maximize area under the curve in the data. To overcome DoubletFinder’s limited sensitivity to homotypic doublets, we consider doublet number estimates based on Poisson statistics with homotypic doublet proportion adjustment assuming 1/50,000 doublet formation rate of the 10X Chromium droplet microfluidic cell loading.
Ambient messenger RNA signal removal
Following doublet prediction and removal we analysed each scRNA-seq dataset to estimate the extent of ambient mRNA contamination in every single cell and correct it. We used the R package Cellular Latent Dirichlet Allocation (CELDA)66 (v.1.16.1), which contains DecontX and is a method based on the Bayesian statistical framework used to computationally estimate and remove RNA contamination in individual cells without empty droplet information. We applied the DecontX() function in CELDA to the raw count matrices with default parameters. Subsequently, we removed all cells with contamination values above 0.5 and used the decontaminated count matrices resulting from DecontX() for downstream analysis.
Geometric sketching
Geometric sketching is a downsampling technique that helps explore and interpret scRNA-seq data more effectively by providing a concise and intuitive representation of the cellular landscape that preserves rare populations. We used the sketchData() function from CellChat67, with default parameters, to select one-third of the sequenced cells for each donor in the homeostatic samples described in Extended Data Fig. 2 (scRNA-seq samples for donor no. 1–3). This strategy was used to avoid variability in sequence efficiencies that influence the computation of lower-dimensional embeddings and heterogeneity analysis.
Data integration
Individual datasets—following preprocessing, doublet removal and ambient mRNA regression—were aggregated according to specific criteria (for example, tissue of origin, profiling time, culture condition) and underwent a joint normalization step with SCTransform to mitigate technical confounding factors, which also served as a means for selection of a meaningful set of the 2,000 most variable global genes before data integration. Integration of different conditions (culture model, treatment and time points) was performed using the log-normalized, corrected UMI count data in two steps. First, the residuals for the top 2,000 global variable genes were used as input in computing the top 100 principal components through the RunPCA() function in Seurat. The 30 leading principal components and 50 nearest neighbours were then used to define the shared neighbourhood graph with the FindNeighbors() function in Seurat. Subsequently, datasets were clustered according to the shared neighbourhood graph using the Louvain algorithm68 through the Seurat function FindClusters(), with resolution 0.8. Finally, we used these high-resolution clusters to define a restricted, noise-reduced and cell state-specific set of genes (‘Differential expression analysis’). In the second step of the integration process we compiled a list consisting of the ensemble of the 30 top differentially expressed genes for each cluster and used it to focus and repeat PCA dimensionality reduction. The first 30 principal component vectors of the new PCA space served as the basis for obtaining a two-dimensional representation of the data through UMAP69 implemented in RunUMAP() with the 50 nearest neighbours. We then computed a shared neighbourhood graph on the UMAP lower-dimensional space and computed the final integrated clusters with resolution parameter 0.2.
Integration index
The integration index of T cells in the homeostatic samples described in Fig. 2 and Extended Data Fig. 2 was computed by identification of the 50 closest neighbours of each T cell on the lower-dimensional space defined by the 30 leading CCA vectors. Subsequently, the proportion of PBMC-derived T cell neighbours was subtracted from the proportion of IIO-derived T cell neighbours of each individual cell and the resulting index was mapped to an interval of 0–1.
Differential expression analysis
Gene differential expression analysis between distinct cell populations in scRNA-seq data was assessed by performing Wilcoxon rank-sum tests and area under the receiving operating characteristic analysis, as implemented by the Presto (v.1.0.0) package in R. log-transformed, corrected UMIs were used as input for differential expression statistical tests, and genes were called differentially expressed if the associated adjusted P value (Bonferroni method) was lower than 0.05, area under the curve value above 0.6 and log fold change greater than 0.15. In addition, we also set thresholds on detection rates of differential expression genes. In particular, a given gene was assigned as overexpressed in the analysed group if it was detected in at least 30% of the samples of that group, whereas detection rate in background samples was at most 70% of the detection rate of the analysed group.
CD8+ T cell activation trajectory reconstruction
For reconstruction of the continuum of the CD8+ T cell activation trajectory in IIO models challenged with bispecific antibodies, we took advantage of diffusion pseudotime as implemented in the destiny package (v.3.14.0) in R. In brief, diffusion pseudotime uses random-walk-based distance, computed on the leading eigenvectors of a transition matrix, to order scRNA-seq data according to differentiation stages44,70. We used the DiffusionMap() function in the destiny package on the space identified by the 30 leading principal component vectors of the integrated PCA embedding of CD8+ clusters. Pseudotime values were then computed with the DTP() function in destiny on the diffusion map object using default parameters. Similarly global pseudotime, following TNF perturbation simulation, was based on a random-walk approach on the cell-state transition matrix.
Intercellular communication analysis
For investigation of ligand–receptor-mediated cell–cell communication during immune cell activation in our IIO models we focused on the signals exchanged between TH1 cells, activated T-bet B cells and CD8+ CTLs. For this analysis we extracted genes labelled as either ligands or receptors from curated databases53 and required that these genes be differentially expressed between the three populations under investigation, which facilitated retrieval of directional information about signal exchange. To gain insights into functional cell–cell communication we used the NicheNet (v.02.01.2000) pipeline, which considers the influence of sender-cell ligands on receiver-cell gene expression53. NicheNet’s analysis pipeline provided us with a ranking of predicted ligands that most probably affect gene expression in activated T-bet B cells and CD8+ CTLs, highlighting the role of critical TH1-secreted factors in driving immune cell phenotypes within IIOs.
Functional enrichment analysis
To gain an understanding of the mechanisms underlying phenotypes in our data, differentially expressed genes were analysed for Gene Ontology biological process enrichment using one-sided hypergeometric testing. P values were adjusted for multiple testing hypotheses by the Bonferroni method, and only those enrichment results below a 5% significance level threshold were considered. For this analysis we considered only those biological processes consisting of sets with more than 10 but fewer than 300 mapped genes.
In silico perturbation analysis
To simulate dynamic shifts in cell identity resulting from ligand signalling cascade activation we used Nichenet’s prior model53. The first step involved the generation of simulated values by applying the gene regulatory network as a function and propagating the relative changes in gene expression following k-nearest-neighbour imputation of the gene expression data. This iterative (three times) signal propagation enabled us to calculate the broad, downstream effects of ligand perturbation, thereby estimating the global transcriptional shift. The estimation of cell-identity transition probability was accomplished by comparing this gene expression shift with that of local neighbours, utilizing a likelihood-based dynamical model. By doing so we could establish a measure of how cell identities transition in response to ligand perturbation. Finally, the transition probabilities were transformed into a weighted local average vector map, encoding the simulated directionality of cell-state transition for each cell. This workflow results from an adaptation and integration of CellOracle71 and scVelo72 in Python (v.3.7).
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
Sequencing files in FASTQ format supporting single-cell transcriptomic analysis of homeostasis conditions, as well as EpCAM TCB treatment, have been deposited in ArrayExpress and are accessible at E-MTAB-14171 and E-MTAB-14170, respectively. Raw count matrices, together with metadata information, for all scRNA-seq samples discussed in this study have been deposited in Mendeley and are available at https://data.mendeley.com/datasets/5h3ym82bnb/1. Source data underlying all graphical representations used in the figures are provided as Supplementary Information. Source data are provided with this paper.
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Acknowledgements
We thank R. Sriram for supporting us with the establishment and oversight of the research agreements governing access to human intestinal specimens. We acknowledge the support of the non-profit foundation HTCR, which holds human tissue on trust, making it broadly available for research on an ethical and legal basis.
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T.R., N.G., J.G.C., L.C. and M. Bscheider conceived the study. T.R., N.G. and J.G.C. wrote the manuscript. T.R., L.S. and M.F.H. were involved in designing and performing most experiments in the manuscript. B.G. and L.A. led the bioinformatics efforts and analysed all scRNA-seq data. T.R., L.S., M. Bellavista, U.K. and D.P. performed scRNA-seq experiments. L.S., M.F.H., K.K., M.P.M. and G.L. performed revision experiments. M.N., R.K. and M. Bickle performed or analysed live-imaging experiments. T.R., L.S., R.G., M.P.M. and B.S. isolated and banked organoids and TRM cells from primary intestinal samples.
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All authors are employees of Hoffmann-LaRoche. The company provided support in the form of salaries for authors but did not have any additional role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
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Extended data figures and tables
Extended Data Fig. 1 Intestinal TRM isolation and comparison to circulating T cells.
a, Comparison of viable CD45+ count in matched donors subjected to either digestion- or crawl out- based isolation. Two-tailed paired T-test. 7 biological replicates. b, Comparison of immune cell proportions subjected to either digestion- or crawl out- based isolation. Values represent percentages of parent population listed above each bar, as determined via flow cytometry. LPL lamina propria lymphocyte, IEL intraepithelial lymphocyte. Mean ± SD of 5 (Digest CD4 LPL, CD4 IEL, CD8 LPL, CD8 IEL, B cells) or 6 (all other conditions) biological replicates. Different symbol shapes represent individual donors. c, Flow cytometry-generated tSNE analysis of one intestinal lymphocyte isolation incorporating lineage-defining immune cell markers. Leftmost plot represents annotated cell populations with smaller plots displaying heatmaps for each of the 10 key surface markers assessed. DP double positive, DN double negative. Data is representative of six biologically-independent replicates. d, Bar charts comparing expression of key activation markers in gut-derived CD4+ and CD8+ T cells isolated via crawl out in cytokine-free or cytokine-supplemented (10IU/ml IL-2, 2 ng/ml IL-15) media in four biological replicates. Two-tailed paired T-test. e, Flow cytometry assessment of key functionality markers in CD4+ T cells (left graph) and CD8+ T cells (right graph) after crawl out isolation. Violin plots collate data from 10 (8 for CD117) independent intestinal tissue resections, 5 (3 for CD117) of which have matched blood-derived comparators, with 1 additional blood only sample. Two-tailed unpaired T-test between gut- and blood-derived expression values for each marker. f, mIF image of an elongated flossing T cell inserting itself between basal-lateral epithelial cell junctions. The 3 images are from the same region: upper image shows the full stain, middle image contains only CD4 and DAPI to emphasize the unusual shape of the T cell, lower image shows only DAPI and cytokeratin to reveal the space in the epithelium generated by the CD4 cell integration. Diameter of the T cell from head to tail is listed. Similar elongated T cells were observed in all IIO cultures analysed by mIF (n = 5). g, Representative mIF image of the IIO culture over time (upon immune cell introduction, 7 and 14 days later). Markers for T cell (CD3) and epithelial cell (ECAD: E-cadherin) identity, and tissue-residence (CD103 and CD69) are included. Data are quantified in (h-j). h, Ratio of epithelial cells to immune cells within identified organoids, during the course of a 14 day IIO culture. Line at median. i-j, Percent of CD3+ T cells expressing CD69 (i) or CD103 (j) during the course of a 14 day IIO culture, as determined by mIF. Data from h-j is derived from 3 biological replicates, with each individual donor represented by a different symbol.
Extended Data Fig. 2 Single-cell transcriptome analysis of homeostatic IIO model.
a, Flow cytometry plots displaying daily CD8+ TRM IEL proportions and GzmB expression in baseline (untreated) IIO cultures after digestion, across 96 h. Plots are representative of 3 independent IIO experiments. b-d, UMAP embeddings of single-cell transcriptome data colored by cell cluster (b), condition (c) and donor origin (d). The sequenced conditions include intestinal organoids containing TRMs (IIO), organoids containing PBMCs (PBMC) and TRMs cultured in matrigel (TRM). e, Dotplot summarizing marker gene expression across different immune (left) and epithelial (right) cell clusters. f, Boxplots displaying the minimum, mean and maximum number of cells across all donors (n = 3) for clusters of epithelial cells cultured with TRMs (IIO) or PBMCs (PBMC). g, Violin plots illustrating the expression of HES1 and ID3 transcription factors in intestinal epithelial stem cells cultured with TRMs or PBMCs. h, UMAP embedding of subsetted T cells colored by condition as portrayed in Fig. 2b. i, Heatmaps representing T cell densities along the reconstructed integration index vector for each condition (rows) and donor (groups) membership. j, Stacked barplot showing the proportion of TRMs cultured with epithelium (IIO), TRMs cultured in matrigel (TRM) and PBMCs cultured with epithelium (PBMC) within 5 equidistant bins of the integration index. k, Dotplot summarizing marker gene expression of TRMs cultured in matrigel (left, bin3) and TRMs cultured with epithelium (right, bin5).
Extended Data Fig. 3 TRMs exhibit rapid and aggressive targeting of healthy epithelium following EpCAM-TCB treatment.
a, Bihourly quantification of caspase 3/7 signal in IIO co-cultures for 68 h treated with either 5 ng/ml EpCAM TCB or a non-targeting control molecule, IIO triplicates representative of 3 independent biological replicates, mean ± SD. Two-tailed unpaired T-test of the area under the curve for each condition. b, Flow cytometry-generated tSNE analysis of all T cells at all timepoints, derived from one representative IIO co-culture and its matched PBMC-organoid co-culture control, treated with 5 ng/ml EpCAM TCB. Plots display heatmaps for each of the 12 surface and intracellular markers assessed by flow cytometry. c, Gating strategy for identifying responder cells (those that expressed TNF-α, IFNγ or GzmB), and the ungating of the concatenated flow cytometry files to reveal the original source of responder T cells. d, Expression of key soluble and cell surface factors in matched TRM and PBMC CD4+ (top row) and CD8+ (bottom row) T cells over 48 h, following IIO or matched PBMC-organoid co-culture treatment with 5 ng/ml EpCAM TCB, as determined via flow cytometry. Mean and SEM, 5 biological replicates (4 for Ki67). Statistically significant values between TRM and PBMC condition at each timepoint are displayed, 2-way ANOVA with Tukey’s Multiple Comparisons Test. e, Cytokine fold-increase in IIO culture supernatants 48 h after 5 ng/mL EpCAM TCB treatment, relative to the matched PBMC-organoid co-culture control, as analysed via Luminex technology. Mean of 3 biological replicates. f, Quantification of caspase 3/7 signal in IIO co-cultures, versus matched organoid-PBMC co-cultures containing either circulating naive and memory T cells for 66 h treated with 5 ng/ml EpCAM TCB, culture quadruplicates representative of 3 independent biological replicates, mean and SD. One-way ANOVA of the area under the curve calculated for each condition. g, Quantification of caspase 3/7 signal in IIO co-cultures containing matched CD103+ and CD103- TRMs for 66 h treated with 5 ng/ml EpCAM TCB or a non-targeting TCB, IIO quadruplicates (triplicates for CD103-ve TRM) representative of 3 independent biological replicates, mean and SD. One-way ANOVA of the area under the curve calculated for each condition. h, Flow cytometry assessment of TNF-α (24 h), IFNγ (48 h) or GzmB (48 h) in CD103+ and CD103- CD4+ T cells (left graph) and CD8+ T cells (right graph) from unsorted IIO cultures after treatment with 5 ng/ml EpCAM TCB. Violin plots collate data from 5 biological replicates. i, Quantification of caspase 3/7 signal in 10-day old IIO co-cultures treated with 5 ng/ml EpCAM TCB or a non-targeting TCB, IIO triplicates (non-targeting) or quadruplicates (EpCAM TCB) representative of 2 biological-independent replicates, mean and SD. Two-tailed unpaired T-test of the area under the curve calculated for each condition.
Extended Data Fig. 4 TRM-generated inflammation promotes recruitment of circulating T cells into the epithelium.
a, mIF images of TRM + PBMC (IIO + PBMC), TRM-alone (IIO) and PBMC-alone co-cultures with intestinal organoids, 18 h post treatment with 100 ng/ml EpCAM TCB treatment. EpCAM+ organoids (red) surrounded by CD3+ TRMs (yellow) and CellTracker CMFDA Green+ CD3+ PBMCs (cyan+yellow). Caspase 3 (green) captures TCB-triggered immune-induced apoptosis, nuclei are stained with DAPI (blue). Scale bar, 100 µm. Images are representative of four independent IIO experiments. b, Immune infiltration of TRMs, PBMC and TRMs+PBMC-organoid into organoids at 30 h post EpCAM TCB treatment. Data represented as boxplots, with whiskers showing all points (minimum to maximum) of the mean number of immune cells per individual organoid, summed and normalized to the sum organoid area for 4 (PBMC alone), 5 (TRM + PBMC) or 6 (TRM alone) independent IIO replicates. One-way ANOVA with Tukey’s multiple comparisons test. c, mIF images of TRM + PBMC, TRM-alone and PBMC-alone co-cultures with intestinal organoids following 4 h treatment with 100 ng/ml EpCAM TCB. Monocytes (yellow) surround organoids (pink), nuclei are stained with DAPI (blue). Scale bar, 100 µm. Images are representative of four independent IIO experiments. d, Luminex quantification of IL-1β, IL-6 and IL-8 in the supernatants of the TRM + PBMC co-culture with organoids and the respective single immune cell condition, 30 h after treatment with 100 ng/ml EpCAM, 3 independent IIO replicates, representative of 3 biologically independent experiments. Mean and SD.
Extended Data Fig. 5 Extended analysis of cell states and interactions in IIOs after treatment.
a, Plot represents IIO CD8+ T cells on the first two diffusion components colored by reconstructed diffusion pseudotime. b, Volcano plot showing variable genes along reconstructed CD8+ T cell trajectory. x-axis represents spearman correlation value between gene expression and pseudotime value, y-axis represents detection rates of genes in the CD8+ T cell populations. c, Expression patterns of most variable TFs (left) and effector genes (right) along the reconstructed CD8+ T cell trajectory. d, Boxplot summarizing the expression pattern of CD8+ CTL (left) and CD8+ Trm IEL (right) gene signatures described in ref. 45 along the reconstructed activation trajectory of IIO CD8+ T cells. x-axis represents 5 equidistant bins of the reconstructed pseudotime. Boxes represent the mean ± 95% CI computed from 100 bootstraps of the cells in each bin. e-g, Barplot showing differentially expressed genes in the CD8+ T cell trajectory (e), in the CD4+ cytotoxic Th1 population (c9) (f) and in the T-bet+ effector B cells (c11) (g) at 4 h and 48 h after EpCAM TCB treatment. h, Heatmap summarizes directed ligand-receptor pairing interactions of T-bet+ effector B cells (c11) and T cell populations (clusters 5 and 9) in the IIO model. i-j, Predicted regulatory potential of CD4+ cytotoxic Th1 cells (c9) ligands towards signature genes of T-bet+ effector B cells (c11) (i) and CD8+ activated IELs (c5) (j).
Extended Data Fig. 6 Inhibition of TNF-α signaling and the ROCK1/2 pathway quells TRM-driven intestinal inflammation.
a, Line graphs quantifying expression of predicted TNF-α targets associated with T cell activation and cytotoxicity, 48 h after treatment with 5 ng/ml EpCAM TCB in the presence of the TNF-α-neutralizing antibody Adalimumab or an isotype control. Left graph displays expression in CD4+ T cells and the right graph displays expression in CD8+ T cells. Two-tailed paired T-test. Data derived from three biological replicates. b, Luminex quantification of CCL2 chemokine in the supernatant of IIO cultures, 48 h after treatment with 5 ng/ml EpCAM TCB in the presence of the TNF-α-neutralizing antibody Adalimumab or an isotype control. Two-tailed paired T-test. Data derived from three biological replicates. c, Luminex quantification of TNF-α in the supernatants of IIO cultures, 24 h and 48 h after treatment with either 5 ng/ml EpCAM TCB or a non-targeting control. Data derived from three biological replicates. Mean and SD. d, Bihourly quantification of caspase 3/7 signal in organoid cultures treated with increasing concentrations of recombinant TNF-α cytokine for 40 h. TRM-containing IIO cultures treated with 5 ng/ml EpCAM TCB served as a positive control for caspase 3/7 induction. Technical triplicates representative of 2 independent biological replicates, mean and SD. e, CellProfiler assessment of TRM migration speed during 1 h live-imaging of IIO cultures, either in the presence of 10 µM ROCKi or vehicle control. Each dot represents a migrating cell tracked for more than 20 frames. Violin plots are a collation of duplicate videos from one IIO culture, n = 471 for vehicle and 546 for ROCKi treated. Similar results were observed in 3 biologically-independent IIO cultures. Bar shows mean. Two-tailed unpaired Mann-Whitney test. f, Line graphs quantifying expression of CD25, GzmB and perforin in CD4+ T cells (left graph) CD8+ T cells (right graph) isolated from IIO co-cultures 72 h after treatment with 5 ng/ml EpCAM-targeting T-cell bispecific antibodies in the presence of 10 µM ROCKi or vehicle control, as determined via flow cytometry. Two-tailed paired T-test, 3 biological replicates. g, Expression of intracellular TNF-α in CD4+ T cells (left graph) CD8+ T cells (right graph) isolated from IIO co-cultures 72 h after treatment with 5 ng/ml EpCAM-targeting T-cell bispecific antibodies in the presence of 10 µM ROCKi or vehicle control. Two-tailed paired T-test, 3 biological replicates, mean and SD h, Viability of T cells within IIO co-cultures 72 h after treatment with 5 ng/ml EpCAM-targeting T-cell bispecific antibodies in the presence of 10 µM ROCKi or vehicle control, as determined via flow cytometry. Cells were first gated based on CD3 expression and defined as viable via the live/dead dye Efluor780. n = 3 biological replicates, mean and SD.
Extended Data Fig. 7 IIO cultures provide a route to study ICI-induced colitis.
a, Bihourly quantification of caspase 3/7 signal in ICI- or isotype- treated IIOs or matched PBMC-organoid co-cultures. 3-independent co-cultures from two biological replicates are shown. Mean and SD. b, LDH signal in the supernatant of the conditions described in a after 48 h of culture. Two-tailed paired T test. c, Flow cytometry quantification of the number of cytotoxic CD38+ Ki67+ GzmB+ CD8+ T cells within the conditions described in a after 48 h of culture. d, Boolean gating-derived flow cytometry plots showing the counts of CD38+ GzmB+ Ki67+ “pathogenic” CD8+ T cells and allogeneic DCs after 48 h of IIO culture in each of the TRM donors, treated with either an isotype control or ICI e, Flow cytometry quantification of the number GzmB+ T cells within the conditions described in a after 48 h of IIO culture. ICI immune checkpoint inhibitor, R1 replicate 1, R2 replicate 2. DC dendritic cell.
Supplementary information
Supplementary Tables
Supplementary Table 1. scRNA-seq of blood- and gut-derived immune cells. The table reports embedding coordinates, annotations and other metadata information for all scRNA-seq data presented in this study. Table 2. Cluster markers for scRNA-seq datasets. The table reports top gene markers for all immune cell populations identified in the scRNA-seq data and described in this study. Table 3. The table reports information on antibodies used for flow cytometry and immunofluorescence analyses. Table 4. All biological material contributing to data plotted in the manuscript; includes information on the precise region of the intestine from which the biological material was derived, and exactly to which data (figures and tables) this material contributed.
Supplementary Video 1
Interaction between epithelial cells and TRM cells within IIOs. Three-dimensional rendering of a video generated from a confocal time-lapse experiment, depicting the dynamic interactions between epithelial cells (blue) and TRM cells (magenta) and the continuous changes in morphology of the latter. Total duration of the video is 1 h; IIOs were imaged at 1 min intervals.
Supplementary Video 2
TRM cell migration within IIOs. Video generated from a confocal time-lapse experiment, depicting the movement of TRM cells (red) in co-culture with autologous human intestinal organoids (not rendered). Migration tracks are shown in cyan. Total duration of the video is 1 h; cells were imaged at 38 s intervals.
Supplementary Video 3
Peripheral blood T cell migration within PBMC–organoid co-cultures. Video generated from a confocal time-lapse experiment, depicting the movement of T cells isolated from PBMCs (red) in co-culture with autologous human intestinal organoids (not rendered). Migration tracks are shown in cyan. Total duration of the video is 42 min; cells were imaged at 38 s intervals.
Supplementary Video 4
Effect of ROCKi on TRM cell motility within IIOs. Video generated from a confocal time-lapse experiment, depicting the movement of TRM cells (red) in co-culture with autologous human intestinal organoids treated with the ROCK inhibitor Y-27632 (10 µM). Total duration of the video is 1 h; cells were imaged at 38 s intervals.
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Recaldin, T., Steinacher, L., Gjeta, B. et al. Human organoids with an autologous tissue-resident immune compartment. Nature 633, 165–173 (2024). https://doi.org/10.1038/s41586-024-07791-5
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DOI: https://doi.org/10.1038/s41586-024-07791-5
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