Abstract
The gill apparatus of gnathostomes (jawed vertebrates) is fundamental to feeding and ventilation and a focal point of classic hypotheses on the origin of jaws and paired appendages. The gill skeletons of chondrichthyans (sharks, batoids, chimaeras) have often been assumed to reflect ancestral states. However, only a handful of early chondrichthyan gill skeletons are known and palaeontological work is increasingly challenging other pre-supposed shark-like aspects of ancestral gnathostomes. Here we use computed tomography scanning to image the three-dimensionally preserved branchial apparatus in Ptomacanthus, a 415 million year old stem-chondrichthyan. Ptomacanthus had an osteichthyan-like compact pharynx with a bony operculum helping constrain the origin of an elongate elasmobranch-like pharynx to the chondrichthyan stem-group, rather than it representing an ancestral condition of the crown-group. A mixture of chondrichthyan-like and plesiomorphic pharyngeal patterning in Ptomacanthus challenges the idea that the ancestral gnathostome pharynx conformed to a morphologically complete ancestral type.
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Introduction
The pharynx of jawed vertebrates (gnathostomes) plays a fundamental role in feeding and respiration. Furthermore, the skeletal pharyngeal arches are central to theories on the origin of jaws and shoulder girdle1,2,3,4, making the pharynx of interest to those seeking the origins of these structures. Information with which to establish its ancestral form can be found in the anatomy of fossilised Palaeozoic gnathostomes: members of now extinct gnathostome groups, as well as early members of the two modern gnathostome clades (the cartilaginous and bony fishes). However, fossils that actually preserve the fragile pharyngeal skeleton are vanishingly rare. Major differences, such as that between the compact, subcranial pharynx of osteichthyans (bony fishes) and the caudally extended pharynx of most chondrichthyans (cartilaginous fishes), remain difficult to reconstruct. Similarly, the polarity of a wealth of phylogenetically important differences in the arrangement of pharyngeal elements are poorly understood. Palaeozoic taxa in which we do understand pharyngeal morphology can prove decisive in resolving these problems and allow us to arbitrate on scenarios of the anatomical and functional evolution of the pharynx5,6,7.
Views of pharyngeal evolution have long been coloured by a perception that modern chondrichthyans are especially primitive2. Palaeontological discoveries have gradually overturned this view, revealing that the superficially basic chondrichthyan anatomy of cartilages and simple scales is in fact derived states8,9,10,11. However, chondrichthyans have continued to be used as proxies for plesiomorphic gnathostome conditions in the pharynx2,3,12, as well as fins13, jaws2,14 and genomes15. Recent investigations using computed tomography scanning have revealed a spate of new data on the gill skeletons of early gnathostomes5,6,16. However, chondrichthyan examples display a pharyngeal skeleton already showing an elasmobranch-like caudal extension. Combined with phylogenetic uncertainty amongst early chondrichthyans this leaves many questions about primitive early chondrichthyan pharyngeal structure open.
Here we use computed tomography to reveal the pharyngeal morphology in an acanthodian-grade stem-chondrichthyan: Ptomacanthus anglicus. Ptomacanthus is from the Lochkovian (419.2–410.8 Mya) of Herefordshire, UK17,18,19, making this the earliest three-dimensionally articulated branchial skeleton known in a gnathostome. The pharynx of Ptomacanthus is organised like that of osteichthyans, lying entirely beneath the neurocranium and covered by a bony operculum. This contrasts with the caudally extended pharynx of most chondrichthyans. Meanwhile the patterning of the skeletal elements in the pharynx of Ptomacanthus comprises a combination of plesiomorphic and chondrichthyan-like character states. We incorporate this new information into a phylogenetic analysis, allowing us to constrain the elongation of the chondrichthyan pharynx to a specific region of the stem-group. We reconstruct an ancestral gnathostome pharynx which displays a mixture of chondrichthyan-like and plesiomorphic states. Ptomacanthus supplies a key reference with which to interpret other early chondrichthyan branchial skeletons, to decipher comparative pharyngeal structure across modern gnathostome groups, and to address current phylogenetic uncertainty deep in the chondrichthyan stem-group.
Results
Pharyngeal morphology in Ptomacanthus
Almost the entire visceral skeleton of Ptomacanthus is preserved: Meckel’s cartilages, the hyoid arch, and five branchial arches on either side (Figs. 1, 2, Supplementary Figs. 1, 3). There is a single, median basihyal, followed by a paired series of ceratohyals and ceratobranchials. Dorsally, on the anatomical left side, there is a complete series of epibranchials preserved, and three partially preserved epibranchials on the right side (likely corresponding to arches 1, 2, and 4). On the left side, a series of largely indistinct mineralisations are present and correspond to pharyngobranchials. There is an empty field between the ceratobranchials, suggesting basibranchial or hypobranchial cartilages were present but not mineralised. However, the ceratobranchial of the fifth arch on the anatomical left-hand side terminates in a particularly large crescentic structure, possibly corresponding to a hypobranchial or basibranchial. The front-most arches, including the first, are extremely crushed and difficult to distinguish from one another, particularly distally. Posteriorly the three-dimensional nature of the arch elements can be seen more clearly (Fig. 2, Supplementary Fig. 1d).
Between the ceratohyals of Ptomacanthus lies a trapezoidal, flattened basihyal (Fig. 2, Supplementary Fig. 1a). It appears to consist of globular calcified cartilage (Supplementary Fig. 2). The anterior corners of the basihyal are closely associated with the ceratohyals, overlying the tip of the left ceratohyal and lying laterally to the right. This suggests that it lay dorsal to the ceratohyals in life as in chondrichthyans5. Posteriorly this basihyal lies next to, and presumably also articulated with, the anteriormost branchial arch (Fig. 2, Supplementary Fig. 1a). There is no evidence for unpaired mineralisations posterior to the basihyal. The close proximity of the first ceratobranchials and the basihyal suggests that hypobranchials were absent in the first branchial arch, as seen in Acanthodes (see Discussion) and Gladbachus6.
The ventral halves of all five of the branchial arches in Ptomacanthus are separate, and comprise a parallel series of long, curved, posteriorly grooved ceratobranchials (Fig. 2b, c, Supplementary Fig. 1a). The arches taper to meet the upper branchial elements – this proximal region is more intact on the anatomical right-hand side of the specimen where a canal running oblique to the direction of the arch is visible on the first and second arches (Supplementary Fig. 1c). On the anatomical right hand side of the branchial skeleton each lower element is divided by a break oblique to the element’s direction (Fig. 2b, c, Supplementary Fig. 1a). On the anatomical left-hand side this division is not apparent, but does correspond with a point at which the arches sharply kink. Distally the arches terminate with box like ends.
Each epibranchial is posteriorly grooved (Fig. 2b, Supplementary Fig. 1a) and curves ventrally to meet its corresponding ceratobranchial. The epibranchials lack any posterior flanges as in some chondrichthyans6. The distal ends of the elements are expanded and appear to link antero-posteriorly – we interpret these as pharyngobranchials, although any separation from their corresponding epibranchials is unclear. The pharyngobranchials are antero-posteriorly ridged and the more anterior elements are smaller, with the anteriormost element being small and capsular in shape. The fifth epibranchial lacks an expanded upper part, and instead articulates with the posterior end of the fourth pharyngobranchial (Fig. 2, Supplementary Fig. 1d, g). On the anatomical right-hand side of the specimen the first, second and third elements are largely preserved as moulds on the surface of the matrix (Supplementary Fig. 3). However, on this side some remnants are left of several epibranchials, and both a posteriorly displaced fourth epibranchial, and a small anterior pharyngobranchial probably corresponding to the left pharyngobranchial 1 are present within the matrix (Fig. 2a, Supplementary Fig. 1e, f).
The dorsal elements of the branchial skeleton are seen mainly on the anatomical left-hand side. The articulations of these dorsal elements with the braincase cannot be directly examined. However, the relatively undisrupted articulation of the elements shows that the dorsal ends of the three anterior-most arches are aligned along the margins of the parachordal plates. This means that they likely had their articulations on or next to the braincase (Fig. 1, Supplementary Fig. 3).
Phylogenetic analysis
Based on these observations, we updated the phylogenetic matrix of Coates et al.6. In the strict consensus tree there is almost no resolution of the chondrichthyan stem-group (Supplementary Fig. 4a). However, in the Adams consensus some consistent branching patterns are revealed. Importantly, the stem-chondrichthyan Gladbachus has moved crownwards on the chondrichthyan stem-group relative to Ptomacanthus (Supplementary Fig. 4b). Filtering these trees reveals that all trees conform to the topology (Stem-gnathostomes(Ptomacanthus(Gladbachus(Crown-chondrichthyans))). The same topology is recovered by the Bayesian analysis (Supplementary Fig. 5). This contrasts with Coates et al. 6 who recovered Gladbachus intercalating the acanthodian grade, as the sister group to all total-group chondrichthyans except the Acanthodii. Our placement of Gladbachus is supported by comparative gill arch characters that have been modified or introduced here (see Supplementary Note 1). All acanthodians are recovered as stem-chondrichthyans, with Ptomacanthus and other climatiid taxa paraphyletic relative to diplacanthids, ischnacanthids and acanthodids, including Acanthodes (Supplementary Figs. 4 and 5). Also recovered in a relatively crownwards position on the stem-group are the seemingly more shark-like taxa Lupopsyrus, Obtusacanthus, Kathemacanthus and Pucapampella, and Doliodus is recovered as the sister taxon to crown-chondrichthyans. Within the crown-group relationships symmoriiformes are recovered as stem-holocephalans, and hybodonts, ctenacanths and xenacanths as stem-elasmobranchs (Supplementary Figs. 4 and 5).
Discussion
The compact pharynx of Ptomacanthus allows us to constrain the evolution of a chondrichthyan-like pharynx to a specific region of the chondrichthyan stem-group. This fits into an emerging picture of early gnathostome relationships, with mounting evidence placing the entire acanthodian grade in the chondrichthyan stem-group6,9,20. Given the presence of a ventral, compact pharynx in both osteichthyans (eg. Raynerius16) and stem-gnathostomes (eg. Paraplesiobatis21) this means that an osteichthyan-like pharynx can be placed at the gnathostome crown-node with a reasonable degree of confidence. The ostensibly osteichthyan-like condition in extant holocephalans is likely homoplasious, given the presence of an elasmobranch-like pharynx in the stem-chondrichthyan Gladbachus, and the probable stem-holocephalan Ozarcus5,6.
Coates et al.6 recovered the Devonian chondrichthyan Gladbachus, which clearly shows an extended elasmobranch-like pharynx, amidst the acanthodian grade on the chondrichthyan stem-group. Contrastingly our reworking of their dataset and the addition of new information on Ptomacanthus to it results in Gladbachus lying crownwards of the acanthodian grade, in part as a result of the morphology of its pharynx. This position is consistent with a host of other characters grouping Gladbachus with the chondrichthyan crown-group to the exclusion of acanthodian-grade taxa, including extensively calcified cartilage, the absence of any kind of large dermal cranial ossification, and the absence of paired fin spines6. This could restrict the evolution of an elasmobranch-like pharynx to somewhere between Ptomacanthus and Gladbachus in the chondrichthyan stem-group. However, the pharynx of the acanthodian-grade stem-chondrichthyan Acanthodes and relatives (acanthodiforms) appears to show the extended elasmobranch-like condition6. Our recovery of Acanthodes in a grade subtending other stem-chondrichthyans including Ptomacanthus means that it is equally parsimonious to assume that the condition either in Acanthodes or Ptomacanthus is apomorphic.
This impasse can be resolved by closer examination of the two pharyngeal conditions in jawed vertebrates, which reveals that they can be split into three morphological variables (Fig. 3). The first of these is the anterior extent of the branchial arches: in osteichthyans (eg. Raynerius Fig. 3b; ref. 16) and stem-gnathostomes (eg. Dicksonosteus Fig. 3a; ref. 22,23.) this lies anteriorly to the parachordal plates, where the anteriormost arches articulate on the basicranium. In the elasmobranch condition there is no articulation between the neurocranium and the anterior branchial arches, and the anteriormost branchial arch lies posterior to the otic capsule (eg. Gladbachus Fig. 3f; ref. 6). Ptomacanthus exhibits a condition more similar to osteichthyans, with a relatively anterior extent of the pharynx (Fig. 3d). Acanthodes also appears to exhibit this condition (Fig. 3c), with Miles7,24 interpreting facets in the basioccipital ossification as having articulated with anterior branchial arches. This aspect of the elasmobranch condition can be constrained on the chondrichthyan stem-group between Ptomacanthus and Gladbachus.
The second aspect that makes a pharynx elasmobranch-like is the degree of posterior extension. In osteichthyans (eg. Raynerius16) and stem-gnathostomes (eg. Paraplesiobatis21) the arches lie mostly ventrally relative to the neurocranium. Contrastingly in the elasmobranch condition they extend well caudally (eg. Ozarcus5). Ptomacanthus corresponds with the osteichthyan condition in this respect, with the branchial arches not extending far past the parachordal plates (Figs. 1 and 3). In Acanthodes, however, the pharynx extends far posterior to the neurocranium, similarly to elasmobranchs25. The conditions in Acanthodes and Ptomacanthus could be equally parsimoniously resolved as being homoplasious, and in most other acanthodian-grade taxa the condition is unclear. A seemingly elongated dermal gill-covering in taxa such as Euthacanthus26,27 and Mesacanthus26 is perhaps indicative of an Acanthodes-like extended pharynx, but the flattened nature of the specimens makes judging the relative positions of parts of the head difficult. One specimen of Diplacanthus clearly shows the branchial skeleton lying ventrally relative to the braincase26. If the extended condition in Acanthodes is indeed apomorphic it is possible that, as Miles28 suggested, it could be linked to its anguilliform body shape: like Acanthodes, eels have an elongate branchial skeleton and a posteriorly displaced shoulder girdle29.
The final defining aspect of an elasmobranch-like pharynx is the absence of an operculum. In osteichthyans and placoderms bony plates cover the gill arches as part of a functionally linked complex of dermal bones enclosing the head and shoulder girdle (eg. Mimipiscis30, Coccosteus31). Contrastingly in living elasmobranchs a series of gill slits are present with no macromeric operculum. Similar gill slits are also often assumed to have been present in shark-like fossil chondrichthyans with an otherwise elasmobranch-like pharynx (eg. Ozarcus5). However, a fleshy operculum like that in living holocephalans may have been present in some early chondrichthyans6,32,33,34. A bony operculum is present in Ptomacanthus17,19 as well as in Acanthodes26 (although much reduced). Bony operculae are also present in many other acanthodian-grade stem-chondrichthyans, although often only forming a partial cover, with dermal ornament demarcating parts of gill slits posteriorly and dorsally (for example, in Climatius and Euthacanthus26). Other stem-chondrichthyans, however, such as Brochoadmones, Kathemacanthus, Doliodus and Gladbachus clearly lack a bony operculum6,35,36,37,38. Bony operculae appear to have been lost in the chondrichthyan stem-group somewhere between Ptomacanthus and these taxa. However, retention of a fleshy operculum cannot be entirely ruled out.
Mapped onto our phylogenetic tree, these character states show the piecemeal evolution of an elasmobranch-like pharynx through the chondrichthyan stem-group. Ptomacanthus demonstrates the osteichthyan-like condition in all three aspects of pharyngeal morphology: these are then lost on the stem-group prior to the divergence of Gladbachus and crown-group chondrichthyans.
The shape and arrangement of components of the pharyngeal skeleton varies in jawed vertebrates, providing morphological characters that can be diagnostic for their relationships5,39. Acanthodes, because of its rare endoskeletal preservation, has been key to interpreting these characters in the very earliest gnathostomes. However, multiple interpretations of the branchial skeleton in Acanthodes exist on account of its unusual pattern of segmentation of largely indistinct tube-like bones7,26,30,40,41. Ptomacanthus helps evaluate the competing interpretations of Acanthodes, adding clarity to the ancestral complement of elements in the gnathostome gill skeleton.
When contextualised Ptomacanthus displays a combination of derived and plesiomorphic pharyngeal traits (Fig. 4). The presence of a basihyal contacting the hyoid and first branchial arch, and the absence of hypohyals and a mineralised posterior basibranchial adds credence to the reconstruction of Acanthodes by Gardiner, which displays identical conditions30. These states occur in both the chondrichthyan stem-group and in the gnathostome stem-group21 suggesting that these morphologies of the ventral pharynx were likely present at the gnathostome crown-node (Fig. 4). The articulation of the ventro-posterior branchial arches seen in Paraplesiobatis and sarcopterygians (Fig. 4) is clearly absent in Ptomacanthus, consistent with either homoplasy or its plesiomorphy for gnathostomes. The dorsal branchial skeleton of Ptomacanthus shows marked similarities to those of other chondrichthyans5,6, with an antero-posterior chain of dorsally ridged pharyngobranchials and five epibranchials, the posteriormost two of which articulate on the same pharyngobranchial. This last condition is comparable to the articulation between the epibranchial IV and the fused epibranchial V and pharyngobranchial IV in Recent chondrichthyans12. This implies a plesiomorphic crown-gnathostome pharynx which, rather than being exclusively chondrichthyan-like, shows a mélange of osteichthyan-like and chondrichthyan-like morphologies.
It has been assumed, implicitly or otherwise, that the ancestral pharyngeal skeleton must have been morphologically complete, with one of every part that we observe in branchial arches (hypo-, cerato-, epi-, pharyngo-, etc.) in every other pharyngeal arch. This has formed the basis of attempts to reconstruct the evolution of the gnathostome jaw suspension using a supposedly morphologically complete hyoid arch with pharyngo- and hypohyals14, as well as in the sporadic revival of the aphetohyoidean hypothesis – the expectation that we should observe a fully functional hyoid gill slit in the earliest gnathostomes5,26,42. However, neither the ancestral gnathostome branchial skeleton we reconstruct here, nor the earliest fossil gnathostome branchial skeletons show any evidence for being morphologically complete. They lack features that might be expected in such a skeleton, for example, hypohyals and pharyngohyals. They also display anatomical conditions that are difficult to interpret in terms of living gnathostome branchial skeletons. This is still consistent with a serial homology between arches. However, the earliest branchial skeletons should not necessarily be expected to be morphologically complete, and nor should apparently morphologically complete branchial skeletons necessarily be expected to represent an instantiation of a plesiomorphic state.
This new information from Ptomacanthus provides an important cipher for interpreting the branchial skeletons of other early gnathostomes. The reorganisation of the chondrichthyan pharynx into an elasmobranch-like configuration can now be constrained to a specific part of the chondrichthyan stem-group, between the divergences of Ptomacanthus and Gladbachus from the crown-chondrichthyan clade. Ptomacanthus allows us to reconstruct an ancestral branchial skeleton which lacked mineralised hypohyals, and which possessed a large median basihyal articulating with the hyoid arch and one branchial arch. Importantly, this reconstructed ancestral skeleton challenges notions of a “morphologically complete” ancestral gnathostome pharynx. While the pharynx of Ptomacanthus is chondrichthyan-like in some respects, many aspects of the elasmobranch pharynx are certainly derived, and care should be taken using the chondrichthyan condition as an ancestral proxy when building scenarios of pharyngeal evolution.
Methods
Specimen
The specimen that we used in this study is Ptomacanthus anglicus17 Natural History Museum, London (NHM) specimen P.24919a, comprising the anatomically ventral half of a siltstone concretion containing the head and pharynx of a fish, and previously described by Miles17 and Brazeau18,19. The part has been etched using hydrochloric acid, leaving moulds of parts of the endoskeleton on the surface17. An x-ray of the specimen revealed that the concretion held additional skeletal material within, leading to this study. The counterpart, NHM specimen P.24919b, was found to contain no significant endoskeletal material.
Geological context
P.24919a was originally collected from a siltstone lenticle in Wayne Herbert Quarry, Herefordshire, UK17,43. This locality is interpreted as having been fluvial on the basis of isotopic and sedimentological evidence44,45. In addition to further specimens of P. anglicus Wayne Herbert has yielded a number of other articulated acanthodians, as well as articulated osteostracans and heterostracans, and pieces of articulated thelodont17,43. The site lies within the Rhinopteraspis crouchi zone, and so is Lochkovian in age (419.2–410.8Mya)43.
Micro computed-tomography scanning
P.24919a was scanned using a Nikon XT H 225 ST at 224 kV and 240 μA, with a 39 μm voxel size. Data were segmented with Mimics v.18 (biomedical.materialise.com/mimics; Materialise, Leuven, Belgium). Segmented models were then imported into Blender (blender.org) to acquire images.
Phylogenetic analysis
Both parsimony and Bayesian analyses were run, on a matrix of 88 taxa and 267 characters. This matrix was adapted from Coates et al.6 with the addition of information on the branchial skeleton of Ptomacanthus, a reworking of characters related to the branchial skeleton, and the addition of two taxa - Paraplesiobatis and Euthacanthus. Details of these modifications are available in Supplementary Note 2.
The parsimony analysis was run in TNT 1.5 (Goloboff and Catalano 2016) using a parsimony ratchet with 10,000 iterations, holding 100 trees each iteration, and using TBR branch swapping. Galeaspida was set as an outgroup and the constraint (Galeaspida (Osteostraci (Mandibulate gnathostomes))) was enforced.
The Bayesian analysis was run in MrBayes v. 3.2.6. Mandibulate gnathostomes were constrained as monophyletic and Galeaspida were set as the outgroup. A flat (uniform) prior was used along with the Mkv model and a gamma-distributed rate parameter. We then carried out the search for 10,000,000 generations, sampling a tree every 1000 generations, and calculated a majority rule consensus tree with a relative burn-in of 25%.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The CT data that this study is based on, as well as 3D models of the described material, are available in figshare, and available here: https://figshare.com/projects/Supplementary_Information_Ptomacanthus_pharynx/59846. All other files underlying this study, including the phylogenetic dataset, additional notes, and videos of the models, are available in the supplementary information files.
References
Gegenbaur, C. Elements of Comparative Anatomy. (Macmillan, London, 1878).
Mallatt, J. Ventilation and the origin of jawed vertebrates: a new mouth. Zool. J. Linn. Soc. 117, 329–404 (1996).
Gillis, J. A., Dahn, R. D. & Shubin, N. H. Shared developmental mechanisms pattern the vertebrate gill arch and paired fin skeletons. Proc. Natl Acad. Sci. USA 106, 5720–5724 (2009).
Gillis, J. A., Modrell, M. S. & Baker, C. V. H. Developmental evidence for serial homology of the vertebrate jaw and gill arch skeleton. Nat. Commun. 4, 1436–1438 (2013).
Pradel, A., Maisey, J. G., Tafforeau, P., Mapes, R. H. & Mallatt, J. A Palaeozoic shark with osteichthyan-like branchial arches. Nature 509, 608–611 (2014).
Coates, M. I. et al. An early chondrichthyan and the evolutionary assembly of a shark body plan. Proc. R. Soc. B Biol. Sci. 285, 20172418 (2018).
Miles, R. S. in Interrelationships of fishes (eds Greenwood, P., Miles, R. S. & Patterson, C.) 63–103 (London, Zoological Journal of the Linnean Society, 1973).
Maisey, J. G. Chondrichthyan phylogeny: a look at the evidence. J. Vertebr. Paleontol. 4, 359–371 (1984).
Zhu, M. et al. A Silurian placoderm with osteichthyan-like marginal jaw bones. Nature 502, 188–193 (2013).
Giles, S., Friedman, M. & Brazeau, M. D. Osteichthyan-like cranial conditions in an Early Devonian stem gnathostome. Nature 520, 82–175 (2015).
Maisey, J. G. The diversity of tessellated calcification in modern and extinct chondrichthyans. Rev. Paléobiol. 32, 355–371 (2013).
Carvalho, M., Bockmann, F. A. & de Carvalho, M. R. Homology of the fifth epibranchial and accessory elements of the ceratobranchials among Gnathostomes: insights from the development of ostariophysans. PLoS ONE 8, e62389 (2013).
Cole, N. J. & Currie, P. D. Insights from sharks: evolutionary and developmental models of fin development. Dev. Dyn. 236, 2421–2431 (2007).
Grogan, E. D. & Lund, R. Debeerius ellefseni (fam. nov., gen. nov., spec. nov.), an autodiastylic chondrichthyan from the Mississippian Bear Gulch Limestone of Montana (USA), the relationships of the Chondrichthyes, and comments on gnathostome evolution. J. Morphol. 245, 219–245 (2000).
Venkatesh, B. et al. Elephant shark genome provides unique insights into gnathostome evolution. Nature 505, 174–179 (2014).
Giles, S., Darras, L., Clément, G., Blieck, A. & Friedman, M. An exceptionally preserved Late Devonian actinopterygian provides a new model for primitive cranial anatomy in ray-finned fishes. Proc. R. Soc. B Biol. Sci. 282, 20151485 (2015).
Miles, R. S. Articulated acanthodian fishes from the Old Red Sandstone of England, with a review of the structure and evolution of the acanthodian shoulder girdle. Bull. Br. Museum 24, 113–213 (1973).
Brazeau, M. D. The braincase and jaws of a Devonian ‘acanthodian’ and modern gnathostome origins. Nature 457, 305–308 (2009).
Brazeau, M. D. A revision of the anatomy of the Early Devonian jawed vertebrate Ptomacanthus anglicus Miles. Palaeontology 55, 355–367 (2012).
Brazeau, M. D. & de Winter, V. The hyoid arch and braincase anatomy of Acanthodes support chondrichthyan affinity of ‘acanthodians’. Proc. R. Soc. B Biol. Sci. 282, 20152210 (2015).
Brazeau, M. D., Friedman, M., Jerve, A. & Atwood, R. A three-dimensional placoderm (stem-group gnathostome) pharyngeal skeleton and its implications for primitive gnathostome pharyngeal architecture. J. Morphol. 278, 1–9 (2017).
Goujet, D. Les Poissons Placodermes du Spitsberg (Cahiers de Paléontologie, Section Vertébrés, Centre National de la Recherche Scientifique, Paris, 1984).
Goujet, D. Dicksonosteus, un nouvel arthrodire du Dévonien du Spitsberg. Remarques sur le squelette viscéral des Dolichothoraci. Colloq. Int. du Cent. Natl. la Rech. Sci. 218, 81–99 (1975).
Miles, R. S. Remarks on the vertebral column and caudal fin of acanthodian fishes. Lethaia 3, 343–362 (1970).
Davis, S. P., Finarelli, J. A. & Coates, M. I. Acanthodes and shark-like conditions in the last common ancestor of modern gnathostomes. Nature 486, 247–250 (2012).
Watson, D. M. S. The acanthodian fishes. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 228, 49–146 (1937).
Newman, M. J., Burrow, C. J., Den Blaauwen, J. L. & Davidson, R. G. The Early Devonian acanthodian Euthacanthus macnicoli from the Midland Valley of Scotland. Geodiversitas 36, 321–348 (2014).
Miles, R. S. Current problems of lower vertebrate phylogeny. In Proc. Fourth Nobel Symp. (ed. Ørvig, T.) 109–127 (Armqvist and Wiksell, Stockholm, 1968).
Nelson, G. J. Gill arches of teleostean fishes of the order anguilliformes. Pac. Sci. 20, 391–408 (1966).
Gardiner, B. G. The relationships of the palaeoniscid fishes, a review based on new specimens of Mimia and Moythomasia from the Upper Devonian of Western Australia. Bull. Br. Mus. 37, 175–418 (1984).
Miles, R. S. & Westoll, T. S. IX. - The Placoderm Fish Coccosteus cuspidatus Miller ex Agassiz from the Middle Old Red Sandstone of Scotland. Part I. Descriptive Morphology. Trans. R. Soc. Edinb. LXVII, 374–476 (1968).
Dick, J. R. F. On the Carboniferous shark Tristychius arcuatus Agassiz from Scotland. Trans. R. Soc. Edinb. 70, 63–109 (1978).
Moy-Thomas, J. A. & White, E. I. On the Palatoquadrate and Hyomandibula of Pleuracanthus sessilis Jordan. Geol. Mag. 76, 459–463 (1939).
Heidtke, U. H. J. Orthacanthus (Lebachacanthus) senckenbergianus FRITSCH 1889 (Xenacanthida: Chondrichthyes): revision, organisation und phylogenie. Freib. Forsch. 481, 63–106 (1999).
Hanke, G. F. & Wilson, M. V. H. in Phylogeny and Paleobiogeography of Fossil Fishes (eds Elliott, D. K., Maisey, J. G., Yu, X. & Miao, D.) 159–182 (Verlag Dr. Friedrich Pfeil, München, 2010).
Hanke, G. F. & Wilson, M. V. H. Anatomy of the early Devonian acanthodian Brochoadmones milesi based on nearly complete body fossils, with comments on the evolution and development of paired fins. J. Vertebr. Paleontol. 26, 526–537 (2006).
Gagnier, P. & Wilson, M. V. H. An unusual acanthodian from Northern Canada: revision of Brochoadmones milesi. Mod. Geol. 20, 235–251 (1996).
Miller, R. F., Cloutier, R. & Turner, S. The oldest articulated chondrichthyan from the Early Devonian period. Nature 425, 501–504 (2003).
Beckett, H. T., Giles, S., Friedman, M. & Eurypterygii, T. Comparative anatomy of the gill skeleton of fossil Aulopiformes (Teleostei: Eurypterygii). J. Syst. Palaeontol. 16, 1221–1245 (2017).
Nelson, G. J. Current problems of lower vertebrate phylogeny. In Proc. Fourth Nobel Symp. (ed. Ørvig, T.) 129–143 (Armqvist and Wiksell, Stockholm, 1968).
Rosen, D. E., Forey, P. L., Gardiner, B. G. & Patterson, C. Lungfishes, tetrapods, paleontology, and plesiomorphy. Bull. Am. Mus. Nat. Hist. 167, 159–276 (1981).
Zangerl, R. & Case, G. Cobelodus aculeatus (Cope), an anacanthous shark from Pennsylvanian black shales of North America. Palaeontogr. Abt. A Palaeozoologie-Stratigr. 154, 107–157 (1976).
White, E. I. The ostracoderm Pteraspis KNER and the relationships of the agnathous vertebrates. Philos. Trans. R. Soc. Lond. B 225, 381–456 (1935).
Schmitz, B., Werdelin, L., Forey, P. L. & Bendix-almgreen, S. E. Sr/ 86 Sr, Na, F, Sr, and La in skeletal fish debris as a measure of the paleosalinity of fossil-fish habitats. Geol. Soc. Am. Bull. 103, 786–794 (1991).
Allen, J. R. L. & Tarlo, L. B. The Downtonian and Dittonian Facies of the Welsh Borderland. Geol. Mag. 100, 129–155 (1963).
Maisey, J. G. The Braincase in Paleozoic Symmoriiform and Cladoselachian Sharks. Bull. Am. Mus. Nat. Hist. 307, 1–122 (2007).
Carr, R., Johanson, Z. & Ritchie, A. The phyllolepid placoderm Cowralepis mclachlani: insights into the evolution of feeding mechanisms in jawed vertebrates. J. Morphol. 270, 775–804 (2009).
Jarvik, E. Middle and Upper Devonian Porolepiformes from East Greenland with special reference to Glyptolepis groenlandica new species and a discussion on the structure of the head in the porolepiformes. Meddr. Grønl. 187, 1–307 (1972).
Heidtke, U. H. J., Schwind, C. & Krätschmer, K. Über die Organisation des Skelettes und die verwandschaftlichen Beziehungen der Gattung Triodus JORDAN 1849 (Elasmobranchii: Xenacanthida). Mainz. Geowiss. Mitt. 32, 9–54 (2004).
Long, J. A. A new Late Devonian acanthodian fish from Mt. Howitt, Victoria, Australia, with remarks on acanthodian biogeography. Proc. R. Soc. Vic. 98, 1–17 (1986).
Acknowledgements
We thank Emma Bernard (Natural History Museum, London, UK) for the loan of the specimen and Tom Davies (University of Bristol, UK) for help and advice scanning it. Zerina Johanson and Patrick Campbell are thanked for access to and training on radiography at NHM. This work was funded by a European Research Council grant awarded to M.D.B. under the European Union’s Seventh Framework Programme (FP/2007–2013)/ERC Grant Agreement number 311092. TNT was made freely available with the sponsorship of the Willi Hennig Society.
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R.P.D. and M.D.B. conceived the project. C.S. X-rayed specimens at the NHM, leading to the study. R.P.D. and M.D.B. collected tomographic data. R.P.D. processed the tomographic data. R.P.D. assembled and analysed the phylogenetic dataset. R.P.D. and M.B.D. interpreted the data and wrote the first draft of the manuscript. All authors discussed and commented on the final version of the manuscript.
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Dearden, R.P., Stockey, C. & Brazeau, M.D. The pharynx of the stem-chondrichthyan Ptomacanthus and the early evolution of the gnathostome gill skeleton. Nat Commun 10, 2050 (2019). https://doi.org/10.1038/s41467-019-10032-3
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DOI: https://doi.org/10.1038/s41467-019-10032-3
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