Abstract
Ornamental orchid breeding programs have been conducted to develop commercially valuable cultivars with improved characteristics of commercial interest, such as size, flower color, pattern, shape, and resistance to pathogens. Conventional breeding, including sexual hybridization followed by selection of desirable characteristics in plants, has so far been the main method for ornamental breeding, but other techniques, including mutation induction by polyploidization and gamma irradiation, and biotechnological techniques, such as genetic transformation, have also been studied and used in ornamental breeding programs. Orchids are one of the most commercially important families in floriculture industry, having very particular reproductive biology characteristics and being a well-studied group of ornamentals in terms of genetic improvement. The present review focuses on the conventional and biotechnological techniques and approaches specially employed in breeding Phalaenopsis orchids, the genus with highest worldwide importance as an ornamental orchid, highlighting the main limitations and strengths of the approaches. Furthermore, new opportunities and future prospects for ornamental breeding in the CRISPR/Cas9 genome editing era are also discussed. We conclude that conventional hybridization remains the most used method to obtain new cultivars in orchids. However, the emergence of the first biotechnology-derived cultivars, as well as the new biotechnological tools available, such as CRISPR-Cas9, rekindled the full potential of biotechnology approaches and their importance for improve ornamental orchid breeding programs.
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References
Ahmad Z, Hassan AA, Idris NA, Basiran MN, Tanaka A, Shikazono N et al. (2006) Effects of ion beam irradiation on Oncidium lanceanum. J Nuclerar Relat Technol 3:1–8
Ahmad S, Chen J, Chen G, Huang J, Zhou Y, Zhao K, Lan S, Liu Z, Peng D (2022) Why Black Flowers? An Extreme Environment and Molecular Perspective of Black Color Accumulation in the Ornamental and Food Crops. Front Plant Sci 13:885176
Ahn CH, Ramya M, An HR, Park PM, Kim YJ, Lee SY et al. (2020) Progress and challenges in the improvement of ornamental plants by genome editing. Plants 9(6):687
Anghelescu NE, Vafaee Y, Ahmadzadeh K, Chen JT (2023) Asymbiotic seed germination in terrestrial orchids: problems, progress, and prospects. In: Tiwari P, Chen JT. (eds) Advances in orchid biology, biotechnology, and omics. Springer, Singapore
Anne S, Lim JH (2020) Mutation breeding using gamma irradiation in the development of ornamental plants: a review. Flower Res J 28:102–115
Azadi P, Bagheri H, Nalousi AM, Nazari F, Chandler SF (2016) Current status and biotechnological advances in genetic engineering of ornamental plants. Biotechnol Adv 34:1073–1090
Bai Y, Ma Y, Chang Y, Zhang W, Deng Y, Zhang N et al. (2023) Identification and transcriptome data analysis of ARF family genes in five Orchidaceae species. Plant Mol Biol 112:85–98
Balilashaki K, Gantait S, Naderi R, Vahedi M (2015) Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity. Physiol Mol Biol Plants 21(3):341–347
Balilashaki K, Zakizadeh H, Olfati JA, Vahedi M, Kumar A, Indracanti M (2019) Recent advances in Phalaenopsis orchid improvement using omics approaches. Plant Tiss Cult Biotech 29(1):133–149
Belarmino MM, Mii M (2000) Agrobacterium-mediated genetic transformation of a Phalaenopsis orchid. Plant Cell Rep 19:435–442
Billore V, Mirajkar SJ, Suprasanna P, Jain M (2019) Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid. Biotechnol Rep 22:e00343
Boutigny A-L, Dohin N, Pornin D, Rolland M (2020) Overview and detectability of the genetic modifications in ornamental plants. Hortic Res 7:11
Cai J, Liu X, Vanneste K, Proost S, Tsai W-C, Liu K-W et al. (2015) The genome sequence of the orchid Phalaenopsis equestris. Nat Genet 47:65–72
Cardoso JC, Vendrame WA (2022) Innovation in propagation and cultivation of ornamental plants. Horticulturae 8:229
Cardoso JC, Zanello CA, Chen JT (2020) An overview of orchid protocorm-like bodies: Mass propagation, biotechnology, molecular aspects, and breeding. Int J Mol Sci 21(3):985
Chai ML, Xu CJ, Senthil KK, Kim JY, Kim DH (2002) Stable transformation of protocorm-like bodies in Phalaenopsis orchid mediated by Agrobacterium tumefaciens. Sci Hortic 96:213–224
Chan Y-L, Lin K-H, Sanjaya, Liao L-J, Chen W-H, Chan M-T (2005) Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack. Transgenic Res 14:279–288
Chao Y-T, Yen S-H, Yeh J-H, Chen W-C, Shih M-C (2017) Orchidstra 2.0—a transcriptomics resource for the orchid family. Plant Cell Physiol 58(1):e9
Chen WH, Tang CY, Kao YL (2010) Polyploidy and variety improvement of Phalaenopsis orchids. Acta Hortic 878:133–138
Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ (2011) Downregulation of putative UDP-glucose: Flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis. Plant Cell Rep. 30:1007–1017
Chen XG, Wu YH, Li NQ, Gao JY (2022) What role does the seed coat play during symbiotic seed germination in orchids: an experimental approach with Dendrobium officinale. BMC Plant Biol 22:375
Chew Y-C, Halim MHA, Abdullah WMANW, Abdullah JO, Lai KS (2018) Highly efficient proliferation and regeneration of protocorm-like bodies (PLBs) of the threatened orchid, Phalaenopsis bellina. Sains Malaysiana 47:1093–1099
Chew Y-C, Abdullah WMANW, Kok D-XA, Ong-Abdullah J, Mahmood M, Lai K-S (2019) Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina. Sains Malaysiana 48:1867–1877
Chin DP, Mishiba K, Mii M (2007b) Agrobacterium-mediated transformation of protocorm-like bodies in Cymbidium. Plant Cell Rep 26:735–743
Chin DP, Mii M, Mishiba KI (2007a) Production of transgenic Phalaenopsis plants by introducing glutathione S-transferase gene into protocorms at an early stage after germination. Acta Hortic 743:101–105
Chin DP, Shiratori I, Shimizu A, Kato K, Mii M, Waga I (2018) Generation of brilliant green fluorescent petunia plants by using a new and potent fluorescent protein transgene. Sci Rep 8(1):16556
Chuang HT, Huang KL, Shen RS, Miyajima I, Hsu ST (2014) Using cut-column pollination method to overcome crossing barriers in Phalaenopsis sunrise goldmour ‘KHM637. J Fac Agric Kyushu Univ 59:265–271
Corte LED, Mahmoud LM, Moraes TS, Mou Z, Grosser JW, Dutt M (2019) Development of improved fruit, vegetable, and ornamental crops using the CRISPR/cas9 genome editing technique. Plants 8(12):601
Cribb P, Schuiteman A (2012) Phalaenopsis-distribution and ecology. Renziana 2:10–13
Davey MR, Anthony P, Patel D, Power JB (2010) Plant protoplasts: isolation, culture and plant regeneration. In: Plant cell culture: essential methods, Wiley-Blackwell, New York, pp 153–173.
Eeckhaut T, Lakshmanan PS, Deryckere D, Van Bockstaele E, Van Huylenbroeck J (2013) Progress in plant protoplast research. Planta 238:991–1003
Eeckhaut T, Van Houtven W, Bruznican S, Leus L, Van Huylenbroeck J (2020) Somaclonal variation in chrysanthemum× morifolium protoplast regenerants. Front Plant Sci 11:607171
Enoki S, Takahara Y (2022) Applications of biotechnological approaches in the product and breeding of Phalaenopsis orchids. In: Khan M(ed) Tropical plant species. IntechOpen, London. https://doi.org/10.5772/intechopen.104597
Fraiture M, Zheng X, Brunner F (2014) An Arabidopsis and tomato mesophyll protoplast system for fast identification of early MAMP-triggered immunity-suppressing effectors. In: Plant-pathogen interactions, Springer, pp 213–230
Gaillochet C, Develtere W, Jacobs TB (2021) CRISPR screens in plants: approaches, guidelines, and future prospects. Plant Cell 33(4):794–813
Grosser JW, Calovic M, Louzada ES (2010) Protoplast fusion technology—somatic hybridization and cybridization. In: Plant cell culture: essential methods, Wiley-Blackwell, New York, pp 175–198
Hossain MM, Kant R, Van PT, Winarto B, Zeng S, Teixeira da Silva JA (2013) The application of biotechnology to orchids. Crit Rev Plant Sci 32:69–139
Hsiao YY, Pan Z-J, Hsu C-C, Yang Y-P, Hsu Y-C, Chuang Y-C et al. (2011b) Research on orchid biology and biotechnology. Plant Cell Physiol 52:1467–1486
Hsiao Y-Y, Chen Y-W, Huang S-C, Pan Z-J, Fu C-H, Chen W-H et al. (2011a) Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids. BMC Genom 12:360
Hsieh K-T, Liu S-H, Wang I-W, Chen L-J (2020) Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2‑oxidase gene. Bot Stud 61:10
Hsing HX, Lin YJ, Tong C-G, Li MJ, Chen YJ, Ko SS (2016) Efficient and heritable transformation of Phalaenopsis orchids. Bot Stud 57:30
Hsu CC, Chen SY, Lai PH, Hsiao YY, Tsai WC, Liu Z-J et al. (2020) Identification of high-copy number long terminal repeat retrotransposons and their expansion in Phalaenopsis orchids. BMC Genom 21:1–13
Hsu C-C, Su C-J, Jeng M-F, Chen W-H, Chen H-H (2019) A HORT1 retrotransposon insertion in the PeMYB11 promoter causes Harlequin/black flowers in Phalaenopsis Orchids. Plant Physiol 180:1535–1548
Huang H, Kuo Y-W, Chuang Y-C, Yang Y-P, Huang L-M, Jeng M-F, Chen W-H, Chen H-H (2021) Terpene synthase-b and terpene synthase-e/f genes produce monoterpenes for Phalaenopsis bellina floral scent. Front Plant Sci 12:700958
Huang JZ, Lin CP, Cheng TC, Chang BCH, Cheng SY, Chen Y-W et al. (2015) A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development. PLoS One 10(5):e0123474
Ichihashi S, Shigemura S (2002) Phalaenopsis callus and protoplast culture. In: Proceedings of the 17th World Orchid Conference, Malaysia, pp 257–261
Julkifle AL, Rathinam X, Sinniah UR, Subramaniam S (2010) Optimisation of transient green fluorescent protein (GFP) gene expression in Phalaenopsis violacea orchid mediated by Agrobacterium tumefaciens-mediated transformation system. Aust J Basic Appl Sci 4(8):3424–3432
Khatun K, Nath U, Rahman M (2020) Tissue culture of Phalaenopsis: present status and future prospects. J Adv Biotechnol Exp Ther 3:273
Kim JB (2020) Current status on applications of conventional breeding techniques and biotechnological system in ornamentals. J Plant. Biotechnol 47:107–117
Kim SH, Kim SW, Ahn J-W, Ryu J, Kwon S-J, Kang B-C et al. (2020b) Frequency, Spectrum, and stability of leaf mutants induced by diverse γ-ray treatments in two cymbidium hybrids. Plants 9(4):546
Kim SH, Jo YD, Ryu J, Hong MJ, Kang BC, Kim JB (2020a) Effects of the total dose and duration of γ-irradiation on the growth responses and induced SNPs of a Cymbidium hybrid. Int J Radiat Biol 96:545–551
Kurniadi AS, Irawati F, Putra SED, Hardjo PH (2023) Induction of protocorm-like bodies (PLBs) Phalaenopsis spp. Hybrids mutation through ultraviolet irradiation (UV254) and Ethyl Methane Sulfonate (EMS). J Appl Agric Sci 7:1–15
Lee H-J, Kim Y-E, Yoon Y-J, Jeong C-S, Lian ML, Paek K-Y et al. (2016) Highly endoreduplicated floral organs of somaclonal variants in clonally propagated Phalaenopsis ‘Spring Dancer. Plant Cell Tiss Organ Cult 126:67–77
Lee Y-I, Tseng Y, Lee Y-C, Chung M-C (2020) Chromosome constitution and nuclear DNA content of Phalaenopsis hybrids. Sci Hortic 262:109089
Lestari EP, Yunus A, Sugiyarto S (2018) Diversity induction of Dendrobium sylvanum orchid through in vitro irradiation of gamma Ray. Biosaintifika J Biol Biol Educ 10:691–697
Li C, Dong N, Zhao Y, Wu S, Liu Z, Zhai J (2021) A review for the breeding of orchids: current achievements and prospects. Hortic Plant J 7:380–392
Li F, Cheng Y, Zhao X, Yu R, Li H, Wang L et al. (2020) Haploid induction via unpollinated ovule culture in gerbera hybrida. Sci Rep. 10(1):1–9
Li J, Kuang P, Liu RD, Wang D, Wang ZN, Huang MR (2013) Transfer of the GAFP and NPI, two disease-resistant genes, into a Phalaenopsis by Agrobacterium tumefaciens. Pak J Bot 45:1761–1766
Li J, Liao X, Zhou S, Liu S, Jiang L, Wang G (2018) Efficient protoplast isolation and transient gene expression system for Phalaenopsis hybrid cultivar ‘Ruili Beauty. Vitr Cell Dev Biol - Plant 54:87–93
Liang C-Y, Rengasamy KP, Huang L-M, Hsu C-C, Jeng M-F, Chen W-H et al. (2020) Assessment of violet-blue color formation in Phalaenopsis orchids. BMC Plant Biol 20:212
Liao L-J, Pan I-C, Chan Y-L, Hsu Y-H, Chen W-H, Chan M-T (2004) Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance. Mol Breed 13:229–242
Liau C-H, You S-J, Prasad V, Hsiao H-H, Lu J-C, Yang N-S et al. (2003) Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid. Plant Cell Rep. 21:993–998
Lin H-Y, Chen J-C, Fang S-C (2018) A protoplast transient expression system to enable molecular, cellular, and functional studies in Phalaenopsis orchids. Front Plant Sci 9:843
Lin YY (2021) Phalaenopsis Harlequin Breeding – Past, Present and Future. Brother Orchid Nursery Co.
Lou Y, Zhang Q, Xu Q, Yu X, Wang W, Gai R, Ming F (2023) PhCHS5 and PhF3'5’H Genes Over-Expression in Petunia (Petunia hybrida) and Phalaenopsis (Phalaenopsis aphrodite) Regulate Flower Color and Branch Number. Plants 12(11):2204
Ma LY, Zhang T (2011) Effects of radiation of 60Co γ-ray on the genetic stability of Phalaenopsis aphrodite. J Anhui Agric Univ 38:802–805
Machmudi M, Purnobasuki H, Utami ESW (2019) The optimization mesophyll protoplast isolation for Phalaenopsis amboinensis. Bulg J Agric Sci 25:737–743
Magdalita PM, Pascual AOS, Villareal RL (2020) Characterization and flowering behavior of eleven philippine native Phalaenopsis species and gamma irradiation effects on Phalaenopsis aphrodite. Philipp J Sci 149:1–10
Magdalita PM, Pascual AOS, Villareal RL (2022) Evaluation of plant and flower characteristics of selected 15-Gy irradiated Phalenopsis aphrodite. Mindanao J Sci Technol 20:236–249
Manchanda P, Kaur A, Gosal SS (2018) Somaclonal Variation for Sugarcane Improvement. In: Gosal SS Wani SH (eds) Biotechnologies of crop improvement, Vol 1, Springer International Publishing Cham, p 299–326
Maziah M, Fern C (2008) Agrobacterium-mediated genetic transformation of Phalaenopsis bellina using GFP and GUS reporter genes. Pertanika J Sci Technol 16:129–139
Melsen K, van de Wouw M, Contreras R (2021) Mutation Breeding in Ornamentals. HortScience 56(10):1154–1165
Mii M, Chin DP (2018) Genetic transformation on orchid species: an overview of approaches and methodologies. In: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols, pp 347–365
Mishiba KI, Chin DP, Mii M (2005) Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination. Plant Cell Rep. 24:297–303
Naing AH, Adedeji OS, Kim CK (2021) Protoplast technology in ornamental plants: current progress and potential applications on genetic improvement. Sci Hort 283:110043
Nakamura N (2010) Dream comes true: development of a blue rose “Applause” and its fragrance. J Jpn Assoc Odor Environ 41:150–156
Noda N, Yoshioka S, Kishimoto S, Nakayama M, Douzono M, Tanaka Y et al. (2017) Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism. Sci Adv 3(7):e1602785
Noman A, Aqeel M, Deng J, Khalid N, Sanaullah T, Shuilin H (2017) Biotechnological advancements for improving floral attributes in ornamental plants. Front Plant Sci 8:530
Nopitasari S, Setiawati Y, Lawrie MD, Purwantoro A, Widada J, Sasongko AB et al. (2020) Development of an Agrobacterium-delivered CRISPR/Cas9 for Phalaenopsis amabilis (L.) Blume genome editing system. AIP Conf Proc 2260(1):060014
Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A, Rahim HA et al. (2016) Principle and application of plant mutagenesis in crop improvement: a review. Biotechnol Equip 30:1–16
Priyadarshan PM (2019) Introduction to Plant Breeding. In: PLANT BREEDING: Classical to modern. Springer, Singapore. https://doi.org/10.1007/978-981-13-7095-3_1
Putri HA, Purwito A, Sudarsono, Sukma D (2021) Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22:1077–1090
Qin X, Liu Y, Mao S, Li T, Wu H, Chu C et al. (2011) Genetic transformation of lipid transfer protein encoding gene in Phalaenopsis amabilis to enhance cold resistance. Euphytica 177:33–43
Raffeiner B, Serek M, Winkelmann T (2009) Agrobacterium tumefaciens-mediated transformation of Oncidium and Odontoglossum orchid species with the ethylene receptor mutant gene etr1-1. Plant Cell Tiss Organ Cult 98:125–134
Rajan RP, Singh G (2021) A review on application of somaclonal variation in important horticulture crops. Plant Cell Biotech. Mol Biol 22:161–175
Ren R, Gao J, Lu C, Wei Y, Jin J, Wong S-M et al. (2020) Highly efficient protoplast isolation and transient expression system for functional characterization of flowering related genes in Cymbidium orchids. Int J Mol Sci 21:2264
Ren R, Gao J, Yin D, Li K, Lu C, Ahmad S et al. (2021) Highly efficient leaf base protoplast isolation and transient expression systems for orchids and other important monocot crops. Front Plant Sci 12:172
Royal Flora Holland (2020) The international orchid register / RHS Gardening. https://apps.rhs.org.uk/horticulturaldatabase/orchidregister/parentageresults.asp?page=2&seedgen=Phalaenopsis
Semiarti E, Indrianto A, Purwantoro A, Isminingsih S, Suseno N, Ishikawa T et al. (2007) Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis. Plant Biotech 24:265–272
Semiarti E, Nopitasari S, Setiawati Y, Lawrie MD, Purwantoro A, Widada J et al. (2020a) Application of CRISPR/Cas9 genome editing system for molecular breeding of orchids. Indones J Biotech 25:61
Semiarti E, Indrianto A, Purwantoro YH, Martiwi INA, Feroniasanti YML, Nadifah F, Mercuriana IS, Dwiyani R, Iwakawa H, Yoshioka Y, Machida Y, Machida C (2010) High-frequency genetic transformation of Phalaenopsis amabilis orchid using tomato extract-enriched medium for the pre-culture of protocorms. J Hortic Sci Biotechnol 85(3):205–210. https://doi.org/10.1080/14620316.2010.11512655
Semiarti E, Indrianto A, Purwantoro A, Machida Y, Machi C (2011) Agrobacterium-mediated transformation of indonesian orchids for micropropagation. In: Genetic Transformation, InTech, pp 215–240.
Semiarti E, Purwantoro A, Puspita Sari I (2020b) Biotechnology approaches on characterization, mass propagation, and breeding of indonesian orchids Dendrobium lineale (Rolfe.) and Vanda tricolor (Lindl.) with its Phytochemistry. In: Mérillon JM, Kodja H (eds) Orchids phytochemistry, biology and horticulture, Springer, pp.299-312
Sherpa R, Devadas R, Bolbhat SN, Nikam TD, Penna S (2022) Gamma radiation induced in-vitro mutagenesis and isolation of mutants for early flowering and phytomorphological variations in Dendrobium ‘Emma White’. Plants 11(22):3168
Shrestha BR, Tokuhara K, Mii M (2007b) Plant regeneration from cell suspension-derived protoplasts of Phalaenopsis. Plant Cell Rep. 26:719–725
Shrestha BR, Chin DP, Tokuhara K, Mii M (2007a) Efficient production of transgenic plants of Vanda through sonication-assisted Agrobacterium-mediated transformation of protocorm-like bodies. Plant Biotech 24:429–434
Sjahril R, Mii M (2006) High-efficiency Agrobacterium- mediated transformation of Phalaenopsis using meropenem, a novel antibiotic to eliminate Agrobacterium. J Hortic Sci Biotech 81:458–464
Sjahril R, Dong PC, Raham SK, Yamamura S, Nakamura I, Amemiya Y et al. (2006) Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method. Plant Biotech 23:191–194
Su V, Hsu BD-D (2003) Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers. Biotech Lett 25:1933–1939
Su V, Hsu BD (2010) Transient expression of the cytochrome p450 CYP78A2 enhances anthocyanin production in flowers. Plant Mol Biol Rep. 28:302–308
Subramaniam S, Rathinam X (2010) Emerging factors that influence efficiency of T-DNA gene transfer into Phalaenopsis violacea orchid via Agrobacterium tumefaciens–mediated transformation system. Int J Biol 2:64–73
Subramaniam S, Vinod B, Sashi S, Rathinam X (2008) Optimization of the transient Gusa gene transfer of Phalaenopsis violacea orchid via Agrobacterium tumefaciens: An assessment of factors influencing the efficiency of gene transfer mechanisms. Adv Nat Appl Sci 2:77–88
Suputri NPAEO, Prasojo IS, Prabowo LAT, Purwestri YA, Purnomo SE (2024) Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system. Braz J Biol 84:e268133
Teixeira da Silva JA, Dobránszki J, Cardoso JC, Chandler SF, Zeng S (2016) Methods for genetic transformation in Dendrobium. Plant Cell Rep. 35:483–504
Thaneshwari T, Kumari P, Aswath C (2018) Haploid and double haploids in ornamentals a review. Int J Curr Microbiol Appl Sci 7(07):1322–1336
Tong C, Wu F, Yuan Y, Chen Y, Lin C (2020) High-efficiency CRISPR/Cas-based editing of Phalaenopsis orchid MADS genes. Plant Biotech J 18:889–891
Tsai CC (2014) A New hybrid genus Amenopsis (Orchidaceae) derived from the cross between Amesiella and Phalaenopsis. Acta Hortic 1025:57–60
Tsai WC, Dievart A, Hsu CC, Hsiao YY, Chiou SY, Huang H, Chen HH (2017) Post genomics era for orchid research. Bot Stud 58(1):1–22
Turnbull C, Lillemo M, Hvoslef-Eide TAK (2021) Global regulation of genetically modified crops amid the gene edited crop boom—a review. Front Plant Sci 12:630396
Utami ESW, Hariyanto S, Manuhara YSW (2018) Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid. J Genet Eng Biotech 16:703–709
Vendrame W, Faria RT, de, Sorace M, Sahyun SA (2014) Orchid cryopreservation. Ciênc Agrotec 38:213–229
Vendrame WA, Khoddamzadeh AA (2016) Orchid biotechnology. Hortic Rev 44:173–228
Vilcherrez-Atoche JA, Iiyama CM, Cardoso JC (2022) Polyploidization in orchids: from cellular changes to breeding applications. Plants 11:469
Vilcherrez-Atoche JA, Silva JC, Clarindo WR, Mondin M, Cardoso JC (2023) In vitro Polyploidization of Brassolaeliocattleya Hybrid Orchid. Plants 12(2):281
Vo T-C, Seo J-W, Kim CK, Kim H-Y, Lim K-B (2019) Breeding of the mini-type Phalaenopsis cultivar ‘yellow green’, having deep-yellow flowers with a red lip. Hort Sci Tech 37(4):540–547
Wang H, Dong B, Jiang J, Fang W, Guan Z, Liao Y et al. (2014) Characterization of in vitro haploid and doubled haploid Chrysanthemum morifolium plants via unfertilized ovule culture for phenotypical traits and dna methylation pattern. Front Plant Sci 5:738
Wang SL, Viswanath KK, Tong C-G, An HR, Jang S, Chen FC (2019) Floral induction and flower development of orchids. Front Plant Sci 10:1–15
Widiarsih S, Dwimahyani I (2013) Gamma irradiation application for mutation breeding in early flowering moth orchid (Phalaenopsis amabilis Bl.). J Ilm Apl Isot Radiasi 9:59–66
Winarto B, Mattjik NA, Purwito A, Marwoto B (2010) Improvement of selected induction culture media on callus induction in anther culture of Anthurium and a histological study on its callus formation. J Nat Indones 12(2):93–101
Wu J-Y, Hsieh T-F, Tsao C-Y, Chuang K-C (2022) Breeding of an Indigo Phalaenopsis by Intergeneric Hybridization: Rhynchonopsis Tariflor Blue Kid ‘1030-4’. HortScience 57(3):489–490
Wu T, Zhao X, Yang S, Yang J, Zhu J, Kou Y et al. (2022) Induction of 2n pollen with colchicine during microsporogenesis in Phalaenopsis. Breed Sci 72:275–284
Xia K, Zhang D, Xu X, Liu G, Yang Y, Chen Z et al. (2022) Protoplast technology enables the identification of efficient multiplex genome editing tools in Phalaenopsis. Plant Sci 322:111368
Yang F, Gao J, Wei Y, Ren R, Zhang G, Lu C-Q et al. (2021) The genome of Cymbidium sinense revealed the evolution of orchid traits. Plant Biotech J 19:2501–2516
Yeung EC (2022) The orchid embryo — “an embryonic protocorm. Botany 100(9):691–706
Yuan SC, Chin SW, Chen FC (2015) Current trends of Phalaenopsis orchid breeding and study on pollen storage. Acta Hortic 1078:19–23
Zanello CA, Cardoso JC (2019) PLBs induction and clonal plantlet regeneration from leaf segment of commercial hybrids of Phalaenopsis. J Hortic Sci Biotech 94:627–631
Zargar M, Zavarykina T, Voronov S, Pronina I, Bayat M (2022) The recent development in technologies for attaining doubled haploid plants in vivo. Agriculture 12(10):1595
Zhang L, Chin DP, Mii M (2010) Agrobacterium-mediated transformation of protocorm-like bodies in Cattleya. Plant Cell Tiss Organ Cult 103:41–47
Zhang YB, Liao FQ, Zhong HQ, Huang PP, Liu TF, Liu ZC (2009a) A preliminary study on pollen Irradiation for Phalaenopsis breeding. Fujian. J Agric Sci 24(3):237–240
Zhang XF, Ren YL, Shang TC, Zhang W (2009b) Effect of 60Co-γ ray irradiation on Protocorm-like Bodies growth of Phalaenopsis. Northern Horticulture
Zhao P, Ren A, Dong P, Sheng Y, Chang X, Zhang X (2018) The antimicrobial peptaibol trichokonin IV promotes plant growth and induces systemic resistance against Botrytis cinerea infection in moth orchid. J Phytopathol 166:346–354
Zheng T, Li P, Li L, Zhang Q (2021) Research advances in and prospects of ornamental plant genomics. Hort Res 8(1):65
Acknowledgements
CMI and JAVA thanks to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. CMI and JCC thank to São Paulo Research Foundation (FAPESP) grant 2020/09426-4 and n° 2018/20673-3. JCC thanks to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for funding the Project 311083/2018-8. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. This work is supported by the USDA National Institute of Food and Agriculture, Hatch project 7001563. The authors would like to especially thank the Editor of Heredity, Prof. Dr. Frank Hailer, for his great efforts and intellectual and editorial contributions to improving the quality of this article.
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CMI: conceptualization, data curation, investigation, methodology, project administration, visualization, writing—original draft. JAV-A: data curation, investigation, validation, writing—review & editing. JCC: conceptualization, validation, funding acquisition, supervision, validation, writing, review, and editing. MAG: writing, reviewing, and editing. WAV: writing, reviewing, and editing.
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Iiyama, C.M., Vilcherrez-Atoche, J.A., Germanà, M.A. et al. Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century. Heredity 132, 163–178 (2024). https://doi.org/10.1038/s41437-024-00671-8
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DOI: https://doi.org/10.1038/s41437-024-00671-8