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The influence of marine fungal meroterpenoid meroantarctine A toward HaCaT keratinocytes infected with Staphylococcus aureus

The Journal of Antibiotics (2024)Cite this article

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Abstract

A new biological activity was discovered for marine fungal meroterpenoid meroantarctine A with unique 6/5/6/6 polycyclic system. It was found that meroantarctine A can significantly reduce biofilm formation by Staphylococcus aureus with an IC50 of 9.2 µM via inhibition of sortase A activity. Co-cultivation of HaCaT keratinocytes with a S. aureus suspension was used as an in vitro model of skin infection. Treatment of S. aureus-infected HaCaT cells with meroantarctine A at 10 µM caused a reduction in the production of TNF-α, IL-18, NO, and ROS, as well as LDH release and caspase 1 activation in these cells and, finally, recovered the proliferation and migration of HaCaT cells in an in vitro wound healing assay up to the control level. Thus, meroantarctine A is a new promising antibiofilm compound which can effective against S. aureus caused skin infection.

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The original data presented in the study are included in the article; further inquiries can be directed to the corresponding author.

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Acknowledgements

This study was carried out using the equipment of the Collective Facilities Center “The Far Eastern Center for Structural Molecular Research (NMR/MS) PIBOC FEB RAS” and using the Collective Facilities Center “Collection of Marine Microorganisms PIBOC FEB RAS”.

Funding

This research was funded by the Russian Science Foundation (grant number 23-24-00471).

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022, Vladivostok, Russia

    Ekaterina A. Chingizova, Artur R. Chingizov, Ekaterina S. Menchinskaya, Evgeny A. Pislyagin, Aleksandra S. Kuzmich, Elena V. Leshchenko, Gleb V. Borkunov, Dmitry L. Aminin & Ekaterina A. Yurchenko

  2. Institute of Cytology RAS, 194064, St. Petersburg, Russia

    Irina V. Guzhova

  3. Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, 80708, Kaohsiung, Taiwan

    Dmitry L. Aminin

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Contributions

Conceptualization, EAY and EAC; methodology, EAY, EAC and ESM; software, EAY, ASK and ANY; validation, DLA, and IVG; formal analysis, ESM, ASK and EAC; investigation, EAC, ARC, ESM, EAP, EVL, GVB, and EAY; resources, IVG and EAC; data curation, DLA and EAY; writing—original draft prep-aration, EAC, and EAY; writing—review and editing, DLA and IVG; visualization, ESM, EAP, and EAC supervision, EAY and DLA; project administration, EAC and EAY; funding acquisition, EAC. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Ekaterina A. Chingizova or Ekaterina A. Yurchenko.

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Chingizova, E.A., Chingizov, A.R., Menchinskaya, E.S. et al. The influence of marine fungal meroterpenoid meroantarctine A toward HaCaT keratinocytes infected with Staphylococcus aureus. J Antibiot (2024). https://doi.org/10.1038/s41429-024-00771-x

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