Abstract
Benastatin K (1), a new chlorinated benastatin derivative, was isolated from the culture broth of the actinomycete Streptomyces sp. HGTA384. The structure of 1 was determined on the basis of spectroscopic analysis, including 1D and 2D NMR, as well as HRESI-MS, UV and IR, and comparison with data reported in the literature. Compound 1 and benastatins A and B exhibited inhibitory activity against Micrococcus luteus (MIC 7.8, 31.3, and 3.9 μM, respectively), and IgE-mediated β-hexosaminidase release in RBL-2H3 cells with IC50 values of 42, 79, and 19 μM, respectively.
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Acknowledgements
This work was supported by JSPS KAKENHI Grant Number JP19K07135 (TK) and the Useful and Unique Natural Products for Drug Discovery and Development (UpRod) Program for Building Regional Innovation Ecosystems at Kumamoto University, Japan. The authors would like to thank Dr. Hari Prasad Devkota, Kumamoto University, for his assistance in improving the English of the manuscript.
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Kawahara, T., Saita, K., Iwamoto, R. et al. Benastatin K, a chlorinated benastatin-related antibiotic from Streptomyces sp. HGTA384. J Antibiot 77, 471–474 (2024). https://doi.org/10.1038/s41429-024-00727-1
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DOI: https://doi.org/10.1038/s41429-024-00727-1