Abstract
Ten new proansamycin B congeners (1–10) together with one known (11) were isolated and characterized on the basis of 1D and 2D NMR spectroscopic and HRESIMS data from the Amycolatopsis mediterranei S699 ΔPM::rifR+rif-orf19 mutant. Compounds 8 and 9 featured with six-membered ring and five-membered ring hemiketal, respectively. Compounds 1, 2, and 9 displayed antibacterial activity against MRSA (methicillin-resistant Staphylococcus aureus), with the MIC (minimal inhibitory concentration) values of 64, 8, and 128 µg/mL, respectively. Compound 1 showed significant cytotoxicity against MDA-MB-231, HepG2 and Panc-1 cell lines with IC50 (half maximal inhibitory concentration) values of 2.3 ± 0.2, 2.5 ± 0.3 and 3.8 ± 0.5 μM, respectively.
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Funding
This research was supported by the National Key Research and Development Program (2019YFA0905402), the National Natural Science Foundation of China (81673317, 81602979, 81530091), the Program for Changjiang Scholars and Innovative Research Team in University (IRT_17R68).
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Ma, X., Ye, F., Zhang, X. et al. Proansamycin B derivatives from the post-PKS modification gene deletion mutant of Amycolatopsis mediterranei S699. J Antibiot 77, 278–287 (2024). https://doi.org/10.1038/s41429-024-00708-4
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DOI: https://doi.org/10.1038/s41429-024-00708-4