Abstract
Three isocoumarins, including two new compounds, (±) pestalactone D (1) and pestapyrone F (2), as well as one known compound, pestapyrone D (3), were isolated from the culture of the endolichenic Pestalotiopsis rhododendri LF-19-12. The planar structures of all compounds were elucidated by NMR and MS spectra. And the absolute configurations of 1 were confirmed by single crystal X-ray diffraction analysis, indicative of it as a racemate of 4S/12S and 4R/12R enantiomers. Compound 1 exhibited weak anti-coronaviral activity against human coronavirus HCoV-229E with an EC50 of 77.61 μM. Based on the bioinformatics analysis, the biosynthetic pathway of 1 has been proposed.
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Acknowledgements
This research was co-funded by The National Natural Science Foundation of China (81973219 and 82104047), and CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1-028).
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Wang, M., Luo, M., Ding, X. et al. (±) Pestalactone D and pestapyrone F, two new isocoumarins from an endolichenic Pestalotiopsis rhododendri. J Antibiot 76, 678–681 (2023). https://doi.org/10.1038/s41429-023-00646-7
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DOI: https://doi.org/10.1038/s41429-023-00646-7