Abstract
Simple, rapid, and accurate detection of Fluoroquinolone (FQ) resistance is essential for early initiation of appropriate anti-tuberculosis treatment regimen among rifampicin-resistant tuberculosis (RR-TB). In this study, we developed a new assay, which combines multienzyme isothermal rapid amplification and a lateral flow strip (MIRA-LF), to identify the mutations on codons 90 and 94 of gyrA for detecting levofloxacin (LFX) resistance. Compared to conventional phenotypic drug susceptibility testing, the new assay detected fluoroquinolone resistance with a sensitivity, specificity, and accuracy of 92.4%, 98.5%, and 96.5%, respectively. Thus, these characteristics of the newly developed MIRA-LF assay make it particularly useful and accurate for detecting FQ resistance in Mycobacterium tuberculosis in resource-limited condition.
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Funding
This study was supported by the projects from National Key Program of Mega Infectious Diseases (Grant No. 2018ZX10302302-001). The funder had no role in the study design, data collection and analysis, decision to publish, or manuscript preparation.
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This study was approved by the Ethics Committee of the National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention. The patients with TB were included only after we received informed written consent from themselves.
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Li, Mc., Lu, Y., Liu, Hc. et al. Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay. J Antibiot 76, 598–602 (2023). https://doi.org/10.1038/s41429-023-00639-6
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DOI: https://doi.org/10.1038/s41429-023-00639-6