Abstract
A new α-pyrone analog, arthrifuranone A (1) was isolated from an EtOAc-extract of Arthrinium pseudosinense culture medium. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using MS and NMR spectroscopic techniques, and the absolute configuration was established by the Mosher’s method and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4 + analysis. The isolated compound was evaluated for its estrogenic activity using the MCF-7 estrogen responsive human breast cancer cells. Compound 1 showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 3.125 μM via phosphorylation of estrogen receptor-α.
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Acknowledgements
This research was funded by Korea University, Korea Polar Research Institute (Grant: PE21150), and the National Research Foundation of Korea (NRF-2019R1A2C1006226 and NRF-2019R1A4A1020626).
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HK and QNN performed the experiments, with the assistance of MWN, KHK, YG, and SHS. JJK offered the fungal source. DL and KSK designed the research. HK, QNN, DL, and KSK wrote the paper.
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Kwon, H., Nguyen, Q.N., Na, M.W. et al. A new α-pyrone from Arthrinium pseudosinense culture medium and its estrogenic activity in MCF-7 cells. J Antibiot 74, 893–897 (2021). https://doi.org/10.1038/s41429-021-00473-8
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DOI: https://doi.org/10.1038/s41429-021-00473-8