Abstract
Cell sorting is important in many studies, from basic science, such as cell biology, to biomedical applications, such as cell engineering and regenerative medicine. We have developed a photoinduced in situ cell detachment system using gold nanoparticle (AuNP)-embedded collagen hydrogels as a spatiotemporal cell sorting system, which is an image-guided cell sorting system. Here, we prepared AuNP-containing collagen coatings as a photosensitive cell scaffold. Various cells cultured on the coating materials were detached by photoirradiation and subsequent medium injection. The monolayer type of AuNP/collagen coatings showed efficient cell detachment with cell damage and may be applied to remove undesired cells. For cell damage suppression, the bilayer-type AuNP/collagen coatings were designed and prepared. We demonstrated the sorting of visually changed transfected CHO-K1 cells and human-induced pluripotent stem cell-derived cardiomyocytes using our system, which is useful for genome editing and regenerative medicine, respectively.
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References
Kojima C, Nakajima Y, Oeda N, Kawano T, Taki Y. Visible laser-induced in situ cell detachment from gold nanoparticle-embedded collagen gel. Macromol Biosci. 2017;17:1600341.
Nakajima Y, Kawano T, Taki Y, Kojima C. Visible light-responsive cell scaffolds with bilayer structures for single cell separation. Res Chem Intermed. 2018;44:4745–54.
Kojima C, Nishio M, Nakajima Y, Kawano T, Takatsuka K, Matsumoto A. Rapid photoinduced single cell detachment from gold nanoparticle-embedded collagen gels with low denaturation temperature. Polymers. 2020;12:213.
Falahati M, Attar F, Sharifi M, Saboury AA, Salihi A, Aziz FM, et al. Gold nanomaterials as key suppliers in biological and chemical sensing, catalysis, and medicine. Biochim Biophys Acta. 2020;1864:129435.
Govorov AO, Richardson HH. Generating heat with metal nanoparticles. Nano Today. 2007;2:30–8.
Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem. 2009;78:929–58.
Djabourov M, Lechaire J-P, Gaill F. Structure and rheology of gelatin and collagen gels. Biorheology. 1993;30:191–205.
Kojima C, Nakajima Y, Kawano T, Takatsuka K. Preparation of a visible light-responsive gold nanoparticle-containing collagen gel microarray for in situ cell separation. Res Chem Intermed. 2021;47:51–60.
Lee JS, Grav LM, Lewis NE, Kildegaard HF. CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives. Biotechnol J. 2015;10:979–94.
Sun N, Longaker MT, Wu JC. Human iPS cell-based therapy: Considerations before clinical applications. Cell Cycle. 2010;9:880–5.
Yagi S, Oeda N, Kojima C. Electroless growth of size-controlled gold nanoparticles using hydroquinone. J Electrochem Soc. 2012;159:H668–73.
Jones KH, Senft JA. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. J Histochem Cytochem. 1985;33:77–9.
Sander JD, Joung JK. CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol. 2014;32:347–55.
Ren C, Xu K, Segal DJ, Zhang Z. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019;37:56–71.
Gullberg DE, Lundgren-Akerlund E. Collagen-binding I domain integrins—What do they do? Progr Histochem Cytochem. 2002;37:3–54.
Ivanova VP, Kovaleva ZV, Zabelinskii SA, Grinchuk TM, Krivchenko AI. Role of collagen tripeptide fragment GER on activation of adhesion and modification of fatty acid composition in membrane phospholipids of CHO-K1 cells. Cell Tissue Biol. 2008;2:115–22.
Karakikes I, Ameen M, Termglinchan V, Wu JC. Human induced pluripotent stem cell-derived cardiomyocytes: Insights into molecular, cellular, and functional phenotypes. Circ Res. 2015;117:80–8.
Acknowledgements
We are grateful to Yusei Takeyasu (Osaka Pref. Univ.) for his help in the preparation of coating substrates.
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KM and TS are inventors of a bioreactor system for culturing human iPS cells and differentiating human iPS cells into cardiac cells. Osaka Prefecture University and Tokyo Women’s Medical University received the research grant from Nikon Corporation.
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Kojima, C., Kanetsuki, A., Nakajima, Y. et al. Cell detachment from monolayer- and bilayer-type gold nanoparticle-containing collagen coatings by visible laser irradiation for cell sorting applications. Polym J 53, 1485–1491 (2021). https://doi.org/10.1038/s41428-021-00527-6
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DOI: https://doi.org/10.1038/s41428-021-00527-6