Abstract
A variety of chemical approaches for the rational design of artificial proteins and peptides have been developed in recent years for the construction of self-assembled nanocapsules. It was previously found that a synthetic 24-mer β-annulus peptide, which participates in the formation of the dodecahedral internal skeleton of the tomato bushy stunt virus capsid, spontaneously self-assembled into artificial viral capsids with a size of 30–50 nm. These artificial viral capsids were established to encapsulate various guest molecules, such as anionic dyes, DNA, quantum dots, and His-tagged proteins. The artificial viral capsids could also be dressed up with gold nanoparticles, single-stranded DNA, coiled-coil spikes, and proteins by modifying with these molecules at the C-terminus of β-annulus peptides. The artificial viral capsids were notably stabilized by dressing up with human serum albumin and acquired enzymatic activity by dressing up with ribonuclease.
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Acknowledgements
This research was partially supported by The Asahi Glass Foundation, a Grant-in-Aid for Scientific Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems” (JSPS KAKENHI Grant No. JP18H04558), and Grant-in-Aid for Scientific Research (B) (JSPS KAKENHI Grant No. JP18H02089). I would like to thank Prof. Nobuo Kimizuka (Kyushu University), Dr Hiroshi Inaba (Tottori University) and all the coworkers, whose names are cited in the references, for their valuable contributions. The author would like to thank MARUZEN-YUSHODO Co., Ltd, for English language editing.
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Matsuura, K. Dressing up artificial viral capsids self-assembled from C-terminal-modified β-annulus peptides. Polym J 52, 1035–1041 (2020). https://doi.org/10.1038/s41428-020-0355-4
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DOI: https://doi.org/10.1038/s41428-020-0355-4