Abstract
Studies have demonstrated the essential functions of microRNAs (miRNAs) in cardiovascular disease. Herein, we explored the roles of miR-126 and miR-223 in the prediction of plaque stability in carotid atherosclerosis (CA).Patients with CA (Nā=ā52) and healthy volunteers (Nā=ā25) were recruited as the study subjects and controls. First, a miRNA microarray was performed to analyze the differentially expressed miRNAs in the serum of normal controls and patients with CA. Next, the correlations of miR-223 and miR-126 expression with plaque stability-related factors were analyzed. Then, the predictive efficacy of miR-223 and miR-126 on plaque stability was analyzed by the ROC curve, and the targeting relationships of miR-223 and miR-126 with COX2 were verified. Finally, the relationship between COX2 expression and CA plaque stability was analyzed. miR-223 and miR-126 were decreased in the serum of CA patients and had good diagnostic efficacy for CA. miR-223 and miR-126 in the serum of CA patients with unstable plaques were lower than that in patients with stable plaques. miR-223 and miR-126 were negatively correlated with plaque instability-related indicators, while COX2, a direct target of miR-223 and miR-126, was positively related to plaque instability-related indicators. Lowly expressed miR-223 and miR-126 in the serum of CA patients can be used as indicators for plaque stability.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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LZ contributed to the study concepts, study design; YW contributed to the literature research; LZ, YW contributed to the experimental studies and data acquisition; LZ, FQ contributed to the data analysis and statistical analysis; LZ contributed to the manuscript preparation. All authors read and approved the final manuscript.
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This study was conducted following the Declaration of Helsinki in 1964 and approved by the ethics committee of Hangzhou Fuyang District Hospital of Traditional Chinese Medicine.
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Zhu, L., Wang, Y. & Qiao, F. microRNA-223 and microRNA-126 are clinical indicators for predicting the plaque stability in carotid atherosclerosis patients. J Hum Hypertens 37, 788ā795 (2023). https://doi.org/10.1038/s41371-022-00760-3
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DOI: https://doi.org/10.1038/s41371-022-00760-3