Abstract
Viral and pharmacological inducers of protein kinase RNA-activated (PKR)-like ER kinase (PERK) were shown to accelerate the phosphorylation-dependent degradation of the IFNAR1 chain of the Type 1 interferon (IFN) receptor and to limit cell sensitivity to IFN. Here we report that hypoxia can elicit these effects in a PERK-dependent manner. The altered fate of IFNAR1 affected by signaling downstream of PERK depends on phosphorylation of eIF2α (eukaryotic translational initiation factor 2-α) and ensuing activation of p38α kinase. Activators of other eIF2α kinases such as PKR or GCN2 (general control nonrepressed-2) are also capable of eliminating IFNAR1 and blunting IFN responses. Modulation of constitutive PKR activity in human breast cancer cells stabilizes IFNAR1 and sensitizes these cells to IFNAR1-dependent anti-tumorigenic effects. Although downregulation of IFNAR1 and impaired IFNAR1 signaling can be elicited in response to amino-acid deficit, the knockdown of GCN2 in melanoma cells reverses these phenotypes. We propose that, in cancer cells and the tumor microenvironment, activation of diverse eIF2α kinases followed by IFNAR1 downregulation enables multiple cellular components of tumor tissue to evade the direct and indirect anti-tumorigenic effects of Type 1 IFN.
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Abbreviations
- eIF2α:
-
eukaryotic translation initiation factor 2-α
- GCN2:
-
general control nonrepressed-2
- IFN:
-
interferon
- IFNAR1:
-
interferon-α/β receptor chain 1
- ISR:
-
integrated stress response
- MEF:
-
mouse embryo fibroblast
- PERK:
-
PKR-like ER kinase
- PKR:
-
protein kinase RNA-activated
- STAT:
-
signal transducer and activator of transcription
- shRNA:
-
short hairpin RNA
- TG:
-
thapsigargin.
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Acknowledgements
We thank M Herlyn, R Kaufman, A Koromilas, A Nebreda, D Ron and Z Ronai for reagents, Dr Yong Zhang for technical help, and the members of Fuchs, Diehl and Koumenis labs for discussion. The support to SB from the ‘Training in Tumor Virology’ Grant 2-T32-CA-557726-06 is greatly appreciated. This work was supported by the NIH Grants CA92900 and CA142425 (to SYF), CA94214 (to CK), CA104838 (to JAD) and by a grant with the Pennsylvania Department of Health (to JAD, CK, and SYF). The Department specifically disclaims responsibility for any analyses, interpretation or conclusions.
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Dr DP Baker is an employee of BiogenIDEC Inc. and owns stock of this company. The other authors declare no conflict of interest.
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Bhattacharya, S., HuangFu, WC., Dong, G. et al. Anti-tumorigenic effects of Type 1 interferon are subdued by integrated stress responses. Oncogene 32, 4214–4221 (2013). https://doi.org/10.1038/onc.2012.439
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DOI: https://doi.org/10.1038/onc.2012.439
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