The search for the holy replicator has been revitalized through ultradeep sequencing of small RNA–primed single-stranded DNA. Saturation sequencing provides an explanation for the lack of overlap in prior data sets, suggests that frequency of origin usage but not site selection is developmentally regulated, and uncovers a complex four-stranded DNA structure associated with most origins.
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References
Gilbert, D.M. Nat. Rev. Genet. 11, 673–684 (2010).
Besnard, E. et al. Nat. Struct. Mol. Biol. 19, 837–844 (2012).
Cayrou, C. et al. Genome Res. 21, 1438–1449 (2011).
Borowiec, J.A. & Schildkraut, C.L. Curr. Opin. Cell Biol. 23, 284–292 (2011).
Blow, J.J. & Ge, X.Q. Genome Biol. 9, 244 (2008).
Nordman, J. & Orr-Weaver, T.L. Development 139, 455–464 (2012).
Gerbi, S.A. & Bielinsky, A.K. Methods 13, 271–280 (1997).
Martin, M.M. et al. Genome Res. 21, 1822–1832 (2011).
Mesner, L.D., Crawford, E.L. & Hamlin, J.L. Mol. Cell 21, 719–726 (2006).
Lucas, I. et al. EMBO Rep. 8, 770–777 (2007).
Cadoret, J.C. et al. Proc. Natl. Acad. Sci. USA 105, 15837–15842 (2008).
Karnani, N., Taylor, C.M., Malhotra, A. & Dutta, A. Mol. Biol. Cell 21, 393–404 (2010).
Sequeira-Mendes, J. et al. PLoS Genet. 5, e1000446 (2009).
Bechhoefer, J. & Rhind, N. Trends Genet. published online, doi: 10.1016/j.tig.2012.03.011 (19 April 2012).
Cayrou, C. et al. Cell Cycle 11, 658–657 (2012).
Remus, D., Beall, E.L. & Botchan, M.R. EMBO J. 23, 897–907 (2004).
Lubelsky, Y. et al. Nucleic Acids Res. 39, 3141–3155 (2011).
Papior, P. et al. J. Cell Biol. (in the press).
Krysan, P.J., Smith, J.G. & Calos, M.P. Mol. Cell. Biol. 13, 2688–2696 (1993).
Acknowledgements
I would like to thank T. Ryba, M. Aladjem, O. Hyrien, E. Besnard and J.M. Lemaitre for assistance with assembling figures and for helpful discussions.
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Gilbert, D. Replication origins run (ultra) deep. Nat Struct Mol Biol 19, 740–742 (2012). https://doi.org/10.1038/nsmb.2352
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DOI: https://doi.org/10.1038/nsmb.2352