Current strategies to modulate protein expression rely on DNA knock out or RNA interference. A novel method — Trim-Away — can directly and rapidly degrade endogenous proteins in mammalian cells, minimizing the risk of phenotype compensation and off-target effects. Trim-Away introduces exogenous TRIM21 (an E3 ubiquitin ligase) and an antibody against the protein of interest into cells via microinjection or electroporation, leading to TRIM21-mediated ubiquitination and degradation by the proteasome. Trim-Away selectively degraded specific proteins in multiple cell types including primary human cells.
References
Clift, D. et al. A method for the acute and rapid degradation of endogenous proteins. Cell 172, 1692–1706 (2017)
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Crunkhorn, S. New method for endogenous protein degradation. Nat Rev Drug Discov 17, 18 (2018). https://doi.org/10.1038/nrd.2017.260
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DOI: https://doi.org/10.1038/nrd.2017.260