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Lung cancer

OSNA enables more accurate detection of micrometastases

Nature Reviews Clinical Oncology volume 15, page 68 (2018)Cite this article

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The presence, or absence, of micrometastases in surgically removed lymph nodes has important implications for the prognosis of patients with non-small-cell lung cancer (NSCLC) following surgery, although standard histopathological methods do not enable the detection of all micrometastases. Now, new research indicates the superiority of the one-step nucleic acid amplification (OSNA) method over histopathological approaches in the detection of micrometastases.

A total of 885 lymph nodes were extracted during 64 different radical surgery procedures, with pooling of lymph nodes originating from the same compartment to create 320 distinct nodal zones. All lymph nodes were examined for the presence of micrometastases using three methods: haematoxylin and eosin staining, OSNA, and immunohistochemistry for cytokeratin 19 (an epithelial cell marker, expression of which is also detected using the OSNA assay).

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References

  1. Vodicka, J. et al. A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method. J. Surg. Oncol. http://dx.doi.org/10.1002/jso.24826 (2017)

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  1. Peter Sidaway
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Sidaway, P. OSNA enables more accurate detection of micrometastases. Nat Rev Clin Oncol 15, 68 (2018). https://doi.org/10.1038/nrclinonc.2017.206

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  • DOI: https://doi.org/10.1038/nrclinonc.2017.206

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