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Cryopreservation and banking of mammalian cell lines

Nature Protocols volume 3, pages 1981–1989 (2008)Cite this article

Abstract

This protocol describes the principles and methods used for the preparation of cryopreserved cell stocks. Following these procedures will ensure the availability of reproducible cultures for use within a single laboratory at different times and for different collaborating laboratories. Although the basic principle is simple, each cell line has characteristics that must to be borne in mind when establishing and testing such stocks. The key requirements are reliable methods for culture, cryopreservation, characterization and quality control.

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Author information

Authors and Affiliations

  1. Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK

    Glyn N Stacey

  2. Department of Surgery, Prostate Cancer Research Centre, Royal Free and University College London Medical School, 67 Riding House Street, London, W1W 7EJ, UK

    John R Masters

Authors
  1. Glyn N Stacey
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  2. John R Masters
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Correspondence to John R Masters.

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Stacey, G., Masters, J. Cryopreservation and banking of mammalian cell lines. Nat Protoc 3, 1981–1989 (2008). https://doi.org/10.1038/nprot.2008.190

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