Abstract
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele–expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor–promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Brady, S.M. et al. Science 318, 801–806 (2007).
Jiao, Y. et al. Nat. Genet. 41, 258–263 (2009).
Farnham, P.J. Nat. Rev. Genet. 10, 605–616 (2009).
Moreno-Risueno, M.A., Busch, W. & Benfey, P.N. Curr. Opin. Plant Biol. 13, 126–131 (2010).
Kaufmann, K. et al. Science 328, 85–89 (2010).
Brady, S.M. et al. Mol. Syst. Biol. 7, 459 (2011).
Reece-Hoyes, J.S. et al. Nat. Methods 10.1038/nmeth.1748 (30 October 2011).
Vermeirssen, V. et al. Nat. Methods 4, 659–664 (2007).
Chen, Y.C., Rajagopala, S.V., Stellberger, T. & Uetz, P. Nat. Methods 7, 667–668 (2010).
Braun, P. et al. Nat. Methods 6, 91–97 (2009).
Deplancke, B., Vermeirssen, V., Arda, H.E., Martinez, N.J. & Walhout, A.J.M. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot4590 (2006).
Shannon, P. et al. Genome Res. 13, 2498–2504 (2003).
Skirycz, A. et al. Plant J. 47, 10–24 (2006).
Acknowledgements
This work was supported by University of California Davis startup funds to S.M.B., US National Institutes of Health grant GM082971 to A.J.M.W., US Department of Agriculture 1907-21000-030 to D.W., GM092412 and GM056006 to S.A.K., We thank K. Cavanaugh, D. Lavelle, L. Williams, P. Nittler, R. Michelmore, J. Pelletier, K. Vo, J. Foret, S. Winte and J. Harada for assistance.
Author information
Authors and Affiliations
Contributions
S.M.B., D.W., A.J.M.W. and J.S.R.-H. conceived the project; J.S.R.-H. and A.J.M.W. provided the eY1H mating protocol; A.G. and D.K. performed the yeast experiments; A.G. and L.Z. cloned the de novo transcription factors; L.Z., M.T.-T., A.G., L.P. and Z.L. recombined the transcription factors; L.Z., G.B., J.L.P.-P. and S.A.K. accumulated and curated transcription factor entry clones; and S.M.B., A.G., D.W., L.Z., A.J.M.W. and J.S.R.-H. wrote the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–3, Supplementary Tables 1–3 and Supplementary Notes (PDF 888 kb)
Rights and permissions
About this article
Cite this article
Gaudinier, A., Zhang, L., Reece-Hoyes, J. et al. Enhanced Y1H assays for Arabidopsis. Nat Methods 8, 1053–1055 (2011). https://doi.org/10.1038/nmeth.1750
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.1750