Abstract
Asthma and autoimmune disease susceptibility has been strongly linked to genetic variants in the 17q21 haploblock that alter the expression of ORMDL3; however, the molecular mechanisms by which these variants perturb gene expression and the cell types in which this effect is most prominent are unclear. We found several 17q21 variants overlapped enhancers present mainly in primary immune cell types. CD4+ T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus, and 4C-Seq assays showed that several distal cis-regulatory elements upstream of the disrupted ZPBP2 CTCF-binding site interacted with the ORMDL3 promoter region in CD4+ T cells exclusively from subjects carrying asthma-risk alleles. Overall, our results suggested that T cells are one of the most prominent cell types affected by 17q21 variants.
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Introduction
Asthma, allergy and autoimmune diseases such as diabetes, Crohn’s disease, ulcerative colitis, psoriasis, rheumatoid arthritis and systemic lupus erythematosus are some of the most common chronic diseases affecting people around the world1,2,3,4,5,6,7,8. Strong evidence of heritability from twin studies has prompted large-scale genome-wide association studies (GWAS) to pinpoint the genetic risk factors that drive the pathogenesis of these complex diseases9. Several thousand common single-nucleotide polymorphisms (SNPs) associated with disease susceptibility have been identified; however, the vast majority of these SNPs are located in non-coding regions of the genome, and thus it has been challenging to define how these SNPs are related to the disease10. Moreover, the cell type(s) where disease risk-associated SNPs have the most prominent effects are unknown, thus hampering functional studies required to successfully translate GWAS discoveries to improvements in disease management. The vast array of immune and structural cell types involved in disease pathogenesis further compound this problem.
Based on recent papers from the ENCODE Project Consortium11,12 and our own analyses13, there is now overwhelming evidence that many disease-associated genetic variants can perturb the functions of cis-regulatory DNA that controls the expression of one or more neighbouring genes on the same allele, thereby influencing gene expression and disease outcome. In many cases these cis-regulatory regions are cell type-specific11, such that a region and its disease-associated genetic variants influence gene expression in just a subset of all possible cell types in the body, and therefore, these susceptible cell types are likely to be major drivers of the genetic risk for that specific disease. Thus, identifying the cell type in which cis-regulatory regions bearing disease-associated SNPs are selectively active may indicate the precise cell types that initiate or maintain disease pathogenesis. For example, by overlapping enhancers of naive and memory CD4+ T-cell subsets with all known asthma-risk SNPs, we recently showed strong enrichment of these SNPs in enhancers of memory CD4+ T cells that produce type 2 cytokines (TH2 cells), implying an important role for TH2 cells in asthma pathogenesis14.
In this study, we take a similar unbiased approach to determine the cell types that are most susceptible to the effects of SNPs located in the 17q12-q21 genetic risk locus. Originally the 17q21 locus was strongly linked to asthma susceptibility, which was confirmed by multiple other GWAS studies in diverse ethnic populations15,16,17,18,19,20 and in a large study of individuals with severe asthma21. Subsequently, various other autoimmune diseases such as type 1 diabetes, Crohn’s disease, ulcerative colitis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus and primary biliary cirrhosis were all found to share the same 17q21 risk locus, suggesting its broader implication in several diseases2,3,4,5,6,7,8,22; however, an important distinction is that the risk alleles for asthma paradoxically have a protective effect for the autoimmune diseases studied2,3,4,5,6,22. Despite the robustness of these associations with asthma and other autoimmune diseases, the molecular mechanisms by which 17q21 SNPs perturb gene expression and/or predispose carriers to disease are unclear.
The 17q21 locus harbours a dense haploblock of 136 SNPs in tight linkage disequilibrium that overlap six gene loci: IKZF3, ZPBP2, GSDMB, ORMDL3, LRRC3C and GSDMA1,23, of which the expression of two genes (ORMDL3 and GSDMB) has been shown to be modestly affected by the asthma-risk SNPs in the locus24,25,26. A recent study that linked genotype to phenotype showed that children carrying the 17q21 asthma-risk SNP (rs7216389) were at higher risk of developing wheeze following rhinovirus infection (common cold), an event that is an independent predictor of asthma development later in life26. Although ORMDL3 expression is elevated in children with asthma as well as in individuals with the 17q21 risk-haploblock1,23,25,27, the biological functions of ORMDL3 that are relevant to asthma pathogenesis are also poorly defined.
Here we systematically examine in which cell types the 17q21 risk haploblock has the most effect on ORMDL3 expression, how that effect is mediated by disruption of cis-regulatory elements, and how cytokine production is thereby affected in T cells. We show that ORMDL3 expression is most perturbed by the 17q21 asthma-risk SNPs in primary T cell subsets and B cells. Assessing H3K27ac (chromatin mark of active enhancers) enrichment levels in the 17q21 locus revealed an active enhancer in the first intron of ORMDL3 that displayed genotype-dependent changes in activity. Further, we discovered that an asthma-risk SNP in this enhancer favours the binding of CTCF (rs4065275), while in contrast another linked SNP downstream of this site prevented CTCF binding (rs12936231), leading to alteration of CTCF-binding patterns in the locus. The three-dimensional (3D) organization of the 17q21 locus in CD4+ T cells was also modified in the asthma-risk alleles to favour recruitment of distal cis-regulatory elements to the ORMDL3 promoter region. Knocking down transcript levels of ORMDL3 in memory CD4+ T cells significantly increased the production of interleukin (IL)-2 following T-cell receptor stimulation.
Results
17q21 asthma-risk SNPs locus overlap immune cell enhancers
The majority of SNPs (94%, 128/136) present in the 17q21 asthma-risk haploblock are located in intronic or intergenic non-coding sequences (non-coding SNPs, ncSNPs) whose cell-specific cis-regulatory potential has not been fully characterized (Fig. 1a,b); as a result, the specific cell type that is most affected by the 17q21 asthma-risk variants is largely unknown. This is especially important for investigating the genetic basis of diseases such as asthma where several immune and structural cells present in the lungs have been implicated in disease pathogenesis28,29,30. Here we utilized the comprehensive DNase I hypersensitivity sites (DHS) data sets, generated by the ENCODE Project31 and NIH Epigenomics Roadmap Consortiums32, to first define the cell types where the 17q21 locus is selectively more active, that is, harbour a significantly higher number of cis-regulatory elements (DHS), as these cell types are more likely to be affected by the ncSNPs present in the locus. A total of 462 unique DHS were present in the 17q21 locus (Fig. 1a) and immune cell types had significantly more DHS when compared with non-immune cell types (P<0.001 by Student’s unpaired two-tailed t-test, and following Bonferroni correction for multiple testing, Fig. 1c and see Methods); this enrichment of immune cell DHS was not observed in many of the other asthma-risk haploblocks identified by GWAS studies (Supplementary Fig. 1a and Supplementary Data set 2), suggesting that this effect in immune cells is not generic for all GWAS asthma-risk haploblocks. By ordering 62 primary cell types based on the number of DHS present, we observed that lymphocytes (T cell subsets, B cells and natural killer (NK) cells) ranked in the top 10, harbouring well over 50 DHS (Fig. 1d), whereas monocytes, lung structural cells including bronchial epithelial cells (BECs), fibroblasts and endothelial cells, and other irrelevant tissue types (for example, brain) harboured relatively fewer DHS. Among fetal tissues, the greatest number of DHS was observed in thymus and spleen (organs rich in immune cells) instead of lung tissue (the target organ affected in asthma) (Supplementary Fig. 1b). Altogether, these data indicate that the locus is highly active in several immune cell types, and that several 17q21 cis-regulatory regions are highly immune cell-specific (Fig. 1a,c,d).
About 17% (23/136) of the SNPs in the 17q21 asthma-risk haploblock directly overlap DHS in the locus (Fig. 1e). Importantly, more than five of the DHS present in immune cells, such as T-cell subsets, B cells and natural killer cells, directly overlap asthma-risk SNPs, whereas DHS present in monocytes, epithelial cells or other irrelevant tissue (brain) overlap one or no asthma-risk SNPs (Fig. 1f). Thus, our in silico analysis of enhancer profiles suggests that expression of 17q21 genes is most likely to be perturbed in immune cell types.
ORMDL3 expression is most affected in primary T cells
In 10 primary immune cell types freshly isolated from peripheral blood of 34 subjects enrolled in the La Jolla Institute for Allergy and Immunology’s (LJI) Normal Blood Donor Program (Supplementary Fig. 2), we assessed expression of two genes in the 17q21 locus (ORMDL3 and GSDMB) whose expression levels have been shown to be affected by 17q21 SNPs in previous studies24,25,26. Compared with non-immune cells, such as BECs, lung cancer cell lines (A549), and human umbilical vein endothelial cells, both genes were expressed at relatively high levels in most primary immune cell types with the exception of monocytes and dendritic cells (Fig. 2a). Among the 34 subjects, expression of ORMDL3 and GSDMB genes were both correlated between the cell types of higher transcriptional activity (Spearman correlation value of 0.74 and 0.83, respectively, between naive CD4+ T cells and CD8+ T cells, Fig. 2b and Supplementary Fig. 3a). Though the expression levels of ORMDL3 and GSDMB were variable across donors (Fig. 2a,b), the two were positively correlated across the 34 donors (Spearman correlation value of 0.63 in naive CD4+ T cells, Fig. 2c), suggesting a potentially co-regulated expression of these two genes in several cell types (Supplementary Fig. 3b).
Based on genotype at rs7216389 (see Supplementary Data set 3)1, located in the gene body of GSDMB, subjects were classified into three groups: (i) homozygous for the asthma-risk allele (TT genotype, n=9), (ii) homozygous for the non-risk allele (CC genotype, n=9) and (iii) carrying heterozygous alleles (TC genotype, n=16). Previous studies have reported a modest increase (∼1.3 to 1.8-fold in peripheral blood mononuclear cells (PBMCs) and lymphoblastoid cell lines)1,24,26,27 in the expression of ORMDL3 and GSDMB transcripts in subjects carrying the asthma-risk alleles. In comparison, we found a much greater increase in purified primary immune cell types, with some cell types such as naive CD4+ T cells and B cells displaying a nearly threefold increase in ORMDL3 expression (Fig. 2d, left panel and Fig. 2e). In sharp contrast, monocytes and dendritic cells showed no genotype-dependent effect on gene expression (Fig. 2d, right panel), implying that the effect of the 17q21 asthma-risk variants is cell-specific and restricted to some primary immune cell types. These findings concur with our prediction based on overlap of 17q21 asthma-risk variants with cell-specific cis-regulatory elements (Fig. 1f).
We noted that the effect of asthma-risk variants on ORMDL3 expression was completely lost when primary naive CD4+ T cells were expanded in culture for a few days (Fig. 2f), suggesting in vitro expansion per se may dampen the effects of the 17q21 variants. Recent studies have shown that 17q21 risk variants have only modest effects on ORMDL3 expression in in vitro expanded human lymphoblastoid cell lines1,27, and in the case of primary BECs these variants exert no effect33. Such divergent effects of the asthma-risk variants among cell types indicate that the upstream pathway positively regulating the ORMDL3 locus is sensitive to the effects of genetic variants only in certain cell types; therefore, the functional consequences of the underlying genetic risk are likely to be best observed in the cell types most sensitive to the effects of asthma-risk variants, in this case T cell subsets. Since CD4+ T cells have a central role in orchestrating asthma pathogenesis34, we focused our functional studies on this subset of T cells.
17q21 SNPs affect function of ORMDL3 intronic enhancer
We next wanted to predict the cis-regulatory elements (enhancers) that are affected by asthma-risk variants present in the 17q21 locus. Among the 23 SNPs that directly overlap 21 DHS in the 17q21 locus (shown in Fig. 1e), we focused our analysis on two groups of SNPs: (i) those that overlap cis-regulatory elements (DHS) selectively enriched in immune cell types where ORMDL3 expression is affected (Fig. 3a, green highlighted boxes), and (ii) those that overlap DHS present in immune cell types as well as a wide range of other primary cell types (Fig. 3a, orange highlighted boxes). The latter SNPs could impact the function of multiple other cell types potentially involved in disease pathogenesis.
Three asthma-risk SNPs (rs4065275, rs8076131 and rs12603332) overlapped DHS in the first intron of ORMDL3 (Fig. 3a, top panel); this ∼3.0 kb intronic region was also highly enriched for the H3K27ac mark, suggestive of active enhancer activity, in CD4+ T cells but not monocytes (Fig. 3a, bottom panel). Next, we asked if the activity of this ORDML3 intronic enhancer, as judged by enrichment levels of H3K27ac, was modified by the asthma-risk alleles. We utilized a large data set of H3K27ac ChIP-Seq assays in memory CD4+ T cells to assess effects of asthma-risk genotype on enhancer activity. An increase (∼1.44 fold) in H3K27ac enrichment was observed in subjects carrying the asthma-risk allele when compared with those carrying the non-risk allele (Fig. 3b), suggesting that the activity of this enhancer is altered by asthma-risk SNPs.
In addition to the ORMDL3 intronic enhancer, two other DHS regions that overlap asthma-risk SNPs were also present in 25–60% of the cell types analysed, that is were not strictly cell-type specific (Fig. 3a, top panel). These regions included the ZPBP2 intronic region that overlaps rs12936231 and the intergenic region that is 25 kb downstream of ORMDL3 intergenic region overlapping ncSNP (rs4795408).
The three DHS sites that were selectively enriched in lymphocytes were present near the IKZF3 gene, some located well over 100 kb away from the ORMDL3 promoter. The distal IKZF3 intergenic region overlaps ncSNP (rs12946510) and similar to the ORMDL3 intronic enhancer, this region showed increased activity (H3K27ac enrichment) in subjects carrying the asthma-risk allele (Fig. 3b,c). The other two regions were (5′) proximal and intronic IKZF3 regions, which overlap the ncSNPs rs2313430 and rs4795397, respectively (Fig. 3b,c). Overall, our observations point to several potentially important enhancer elements in the 17q21 locus, especially the ORMDL3 intronic enhancer, whose function is affected by asthma-risk variants.
17q21 asthma-risk SNPs switch CTCF-binding sites
We performed an in silico analysis to identify the transcription factors whose binding to the cis-regulatory regions described above is perturbed by asthma-risk SNPs. Our evaluation pointed to perturbation of two CTCF-binding motifs as well as motifs of several other transcription factors such as IRF1, PAX4, TCF3 (Fig. 4a and Supplementary Data set 5). We focused on CTCF binding because it directly overlaps the ORMDL3 intronic enhancer region (Fig. 4a), and CTFC protein has been shown to play an important role in enhancer–promoter interactions and as an insulator protein35. The first CTCF-binding motif was altered by an asthma-risk SNP (rs4065275) in the ORMDL3 intronic region causing an A-to-G change in the motif, which is predicted to enhance the binding of CTCF (Fig. 4a and Supplementary Data set 5). We experimentally confirmed that CTCF binding in the ORMDL3 intronic region was significantly enriched in both expanded and primary T cells from subjects carrying the risk allele (Fig. 4b). As a control region, we selected a well-known invariant CTCF binding site ∼50 kb proximal to ORMDL3 (near the GSDMA gene), which did not harbour any motif-disrupting asthma-risk SNP; this region did not show any significant change in CTCF binding (Supplementary Fig. 4a). In heterozygous subjects, we performed allele-specific analysis of CTCF-bound DNA and found strong preference for the risk allele (Fig. 4c and Supplementary Fig. 4b). Thus, we confirmed that the presence of asthma-risk SNP (rs4065275) introduces a CTCF-binding site in the ORMDL3 intronic region that harbours an active enhancer.
The second CTCF-binding motif was altered by asthma-risk SNP (rs12936231) in the ZPBP2 intronic region causing a G-to-C change in the CTCF motif, which was predicted in our in silico analysis and previously shown to impair CTCF binding27. We confirmed in CD4+ and CD8+ T cells that CTCF binding was almost completely lost in the ZPBP2 intronic region from subjects carrying the asthma-risk SNP, and this finding was true at the allele-specific level (Fig. 4b,c). Thus, the asthma-risk haploblock harbours linked SNPs that switch the binding site of CTCF from the ZPBP2 to the ORMDL3 intronic region (Fig. 4d).
17q21 SNPs modify ORMDL3 promoter–enhancer interactions
Since CTCF has a major role in long-range interactions of cis-regulatory elements to cognate promoter regions36,37,38,39, we hypothesized that the switching of CTCF-binding sites may affect the 3D architecture (organization) of the 17q21 locus to favour enhanced transcription of ORMDL3 in the asthma-risk alleles. In order to define all the distal cis-regulatory elements that potentially regulate ORMDL3 expression in an asthma-risk genotype-dependent manner, we performed 4C-Seq assays (see Methods) in primary CD4+ T cells from subjects homozygous for the risk (n=4) and non-risk alleles (n=4). By designating the ORMDL3 promoter as the bait for 4C, we detect at a high resolution the physical interactions of any potential cis-regulatory element in the extended 17q21 locus with the ORMDL3 promoter region (Fig. 5a,b). The 4C-Seq assay40,41 conditions were initially optimized in a human T cell line (HUT-78) to robustly detect long-range interactions of the ORMDL3 promoter (Supplementary Fig. 5a and see Methods).
Genomic regions interacting with the ORMDL3 promoter region were determined using two complementary analysis methods for 4C data (4C-ker42 and 4Cseqpipe40, see Methods). We first pooled data from all risk and non-risk subjects and identified several interacting regions including the CTCF-bound regions near ZBPB2 and ORMDL3 gene loci, the IKZF3 promoter region and a number of other potential enhancer regions in the IKZF3 intronic region (Fig. 5c), suggesting extensive long-range interactions of the ORMDL3 promoter region in primary human CD4+ T cells. Most strikingly, the IKZF3 promoter region and other potential enhancers in the IKZF3 intronic region that were enriched for H3K27ac and H3K4me1 marks in CD4+ T cells but not monocytes (that is, selectively active in CD4+ T cells) appeared to interact with the ORMDL3 promoter region exclusively in subjects carrying the 17q21 asthma-risk allele (Fig. 5c, red highlighted boxes; for example, region chr17: 38,022,212-38,022,898 (hg19) with an adjusted P value of 0.000736 using the 4C-ker method42, Supplementary Data set 6), whereas the ORMDL3 promoter region of non-risk alleles interacted with the CTCF-binding site in the ZPBP2 intronic region (Fig. 5c, blue highlighted boxes), which presumably insulated (blocked) the interactions to the adjacent active IKZF3 cis-regulatory elements. Though subjects in each group showed some variability, potentially due to their genotypic variation within this locus as we classified subjects as risk and non-risk only based on genotype at rs7216389, rs4065275 and rs12936231 (see Methods), the overall pattern was consistent across most donors (Supplementary Fig. 5b and Supplementary Data sets 6 and 7).
Our results suggest that the 3D organization of the 17q21 locus is modified in memory CD4+ T cells from subjects with the 17q21 asthma-risk alleles to favour selective recruitment of the IKZF3 cis-regulatory elements to the ORMDL3 promoter region (Fig. 5d), which correlated with its increased transcriptional activity.
ORMDL3 negatively regulates IL-2 production by CD4+ T cells
Next, we wanted to determine the functional consequences of altered levels of ORMDL3 transcripts in primary CD4+ T cells, as 17q21 SNPs had the greatest impact on ORMDL3 expression in this cell type. ORMDL3 is an endoplasmic reticulum membrane protein, and studies in cell lines and in epithelial cells have indicated its involvement in Ca2+ signalling, sphingolipid metabolism and unfolded-protein responses43,44,45; however, its function in primary T cells has not been fully investigated. Here we sought to determine the functional role of ORMDL3 in memory CD4+ T cells with a specific focus on cytokines released following activation. Freshly isolated human memory CD4+ T cells were transfected with small interfering RNA (siRNA) pools targeting the ORMDL3 transcript, cultured for 48 h to achieve adequate gene knockdown, and then activated by T cell receptor ligation (anti-CD3) and co-stimulation (anti-CD28) to study effects on cytokine production (Fig. 6a and see Methods).
We achieved over 50% reduction in ORMDL3 transcript levels without affecting cell viability due to transfection (Fig. 6b and Supplementary Fig. 6a,c). Among the cytokines and chemokines measured, the strongest effect (>40% change) was seen for IL-2 production, which was significantly increased in the ORMDL3 siRNA-treated conditions compared to control siRNA conditions (Fig. 6c). IL-2 production was not affected by knocking down GSDMB or other control genes such as TBX21 and GATA3 (Fig. 6d).
At the transcriptional level, we observed similar effects on activation-induced expression of IL2 transcripts following ORMDL3 knockdown (Fig. 6e). Notably, we observed significant effects at very early (3, 6 h) and late (18 h) time points following stimulation, suggesting sustained effects of ORMDL3 knockdown (Fig. 6e, right panel). We also confirmed these effects at the protein level using fluorescence-activated cell sorting (FACS)-based intracellular cytokine detection assay (Fig. 6f). We next asked if the negative regulatory effect on IL2 transcription was also observed in unmanipulated T cells from subjects with varying levels of ORMDL3 expression. As expected, we found a striking correlation between baseline ORMDL3 expression and the levels of IL2 transcripts produced following brief ex vivo stimulation of memory CD4+ T cells with phorbol myristate acetate (PMA) and Ionomycin (Fig. 6g), suggesting that this correlation may also hold in vivo. Altogether, our results provide strong evidence that ORMDL3 negatively regulates IL-2 production in CD4+ T cells, and that overexpression of ORMDL3 in subjects carrying the asthma-risk alleles is likely to reduce IL-2 production in vivo, which in turn may have important functional effects that contribute to the development of asthma46.
ORMDL3 knockdown also led to modest changes in the production of other cytokines such as interferon-γ (IFN-γ), IL-10 and IL-13, suggesting that ORMDL3 is likely to have a broader spectrum of functions in T cells. Knocking down GSDMB led to modest changes in the production of TNF, IL-13 and IL-16 (Fig. 6h), suggesting that the product of this gene is also likely to affect the function of T cells. Overall, our results suggest that physiological alteration in the levels of ORMDL3 and GSMDB induced by the asthma-risk allele may be sufficient to modulate the functional capacity of T cells.
Discussion
The overwhelming majority of the sequence variants (SNPs) associated with disease-risk haplotypes in GWAS studies do not change protein coding, implying that a proportion of them may be located in regulatory regions which act in cis to alter gene expression1,16,20,47. Since in many instances cis-regulatory regions are highly cell type-specific, the effects of SNPs that affect their function are also likely to be more pronounced in just a few cell types. The failure to link non-coding risk SNPs to the cell types in which they affect function has significantly hampered the progress of functional studies aimed at identifying biological effects of GWAS SNPs. In this study, we attempted to solve this problem by taking a comprehensive approach to predict and test the effects of 17q21 asthma-risk SNPs on expression of ORMDL3 in various cell types. We show that primary immune cell types, with the exception of monocytes and dendritic cells, but not lung structural cells such as BECs, are most sensitive to the effects of 17q21 SNPs. Notably, this approach allowed us to narrow down the cell types in which ORMDL3 potentially influences disease outcomes, and, therefore, perform detailed functional studies in a relevant cell type (primary human CD4+ T cells) and show that ORMDL3 negatively regulates the transcription of IL2. Given the pleotropic role of IL-2 in modulating the differentiation and function of TH cell subsets, it is likely that this effect on IL-2 production could be pivotal for driving genetic risk for asthma and autoimmunity.
Previous studies have examined the function of the conserved ORM family proteins in yeast48,49 (which includes ORMDL3 in humans), as well as ORMDL3 itself in various mammalian cell lines and in primary cell types relevant to asthma pathogenesis such as epithelial cells, eosinophils, mast cells and whole PBMCs23,43,44,45,50,51,52,53,54. ORM family proteins were originally shown to mediate sphingolipid metabolism in yeast48,49, which was subsequently confirmed in mammalian cells44. As an endoplasmic reticulum membrane protein, ORMDL3 has been shown to be involved in endoplasmic reticulum-mediated Ca2+ homeostasis by depleting Ca2+ stores of the endoplasmic reticulum through an interaction with the SERCA pump in the endoplasmic reticulum43,50. In this context, ORMDL3 was shown to diminish the translocation of the nuclear factor of activated T cells (NFAT) in Jurkat cell lines and modestly reduce cytokine release after stimulation50. Therefore, it is likely that the negative regulatory effects of ORMDL3 on IL-2 production in primary T cells are also due to perturbation of Ca2+ signalling and NFAT function following T cell activation.
Overexpression of ORMDL3 in BECs has been shown to activate components of endoplasmic reticulum stress or unfolded protein response, resulting in increased expression of several pro-inflammatory molecules involved in airway remodelling and inflammation45,51. Recent studies in murine primary eosinophils and mast cells have revealed opposing functions in these cell types; in eosinophils ORMDL3 positively regulates its activation and function by influencing cell shape change, adhesion and recruitment to sites of inflammation in vivo, while in mast cells ORMDL3 negatively regulates activation-induced expression of pro-inflammatory mediators and chemotactic responses51,53. Thus, it appears that the function of ORMDL3 may vary considerably depending on the cell type, and, therefore, it will be important to first know whether the 17q21 asthma-risk SNPs in fact affect the expression of ORMDL3 in these cell types; this information will allow determination of whether these cell types are the dominant factors driving the 17q21-related genetic risk of asthma.
While all SNPs in genetic linkage may be statistically significantly associated with asthma, only a subset will be functionally relevant. For instance, the 17q21 locus has a dense haploblock of 136 SNPs linked to asthma risk, a number too large to allow exploration of each SNP’s potential function. In determining which SNPs are likely to influence pathophysiology, our basic assumption is that a significant fraction of ncSNPs that are deemed to be functional in asthma act on cis-regulatory elements that are important for controlling the expression of 17q21 genes. Therefore, to identify functional SNPs in the 17q21 haploblock, we first focused on SNPs that overlap cis-regulatory regions that are active in several cell types, especially those (for example, CD4+ T cells) in which expression of ORMDL3 is affected by the 17q21 SNPs, and then tested whether the presence of these SNPs alters epigenetic profiles, that is, activity at relevant cis-regulatory DNA regions. We identified an enhancer region in the first intron of the ORMDL3 gene that overlapped three 17q21 asthma-risk SNPs (rs4065275, rs8076131, and rs12603332), and this region showed increased activity in subjects carrying the asthma-risk allele. The rs4065275 variant introduced a binding site for the multi-functional zinc finger protein CTCF, whereas the linked rs12936231 variant, about 55 kb downstream of ORMDL3, disrupted a second CTCF-binding site, which together results in interchanging of two CTCF sites in the vicinity of the ORMDL3 gene locus.
CTCF binds insulator regions in the genome55,56,57, where it forms gene boundaries by blocking interactions between distal enhancers and promoters; when present in introns, it influences the efficiency of pre-mRNA splicing at weak splice sites by altering the processivity of RNA polymerase II (pol II)35; however, we did not observe any changes in splicing of ORMDL3 transcripts (data not shown). More recently, genome-wide analysis of CTCF-binding patterns coupled with long-range chromatin interaction studies (ChIA-PET) have shown that CTCF has a pivotal role in promoting interaction of multiple enhancers to cognate gene promoters58. Therefore, we hypothesized that the switching of CTCF sites by asthma-risk SNPs may alter the three-dimensional structure of chromatin in the 17q21 locus and promote the interaction of active enhancer regions with their cognate promoter (ORMDL3), thereby increasing ORMDL3 expression in carriers of the asthma-risk SNPs. Experimentally, we confirmed genotype-dependent differential looping (altered 3D organization) in the 17q21 locus that resulted in the recruitment of active cell-specific cis-regulatory elements (for example, of the 5′ IKZF3 proximal region which overlaps the asthma-risk SNP rs4795397) to the ORMDL3 promoter in asthma-risk alleles. Depending on the composition of the haploblock for a given donor (combination of SNPs), the differential looping may also recruit enhancer regions that themselves are affected by SNPs and thus creating variable and donor-specific reorganization of the 17q21 locus. These mechanisms may contribute to the exquisite cell type-specific effects of 17q21 SNPs on gene expression in people with various genotypes.
The discovery of new drug targets in a human disease requires identification of the pathogenic cell type(s) as well as the molecular factors and pathways that contribute to the disease. Our current knowledge of these points is incomplete for many diseases. The approach we have taken to improve our understanding of the genetic basis of asthma and autoimmunity aims to address some of these issues and may be applied to various other disease-associated genetic loci to unravel disease-relevant cell types and molecular pathways.
Methods
Study subjects and sample processing
Blood samples from 34 donors were obtained from LJI’s Normal Blood Donor Program after written informed consent. Ethical approval for the use of this material was obtained from the Institutional Review Board (IRB) of the LJI. Only for the data shown in Fig. 3b,c, additional samples were obtained from another cohort of 38 donors (only subjects homozygous for either the asthma risk- or non-risk variant at rs7216389), after written informed consent. The genotyping of the 17q21 SNPs rs7216389, rs12936231 and rs4065275 was performed using the automated Sanger DNA sequencing platform (GENEWIZ, Inc.) with the primers listed in Supplementary Table 1. Details of the genotype of 17q21 SNPs, age and gender of study subjects are provided in Supplementary Data set 3.
For isolating immune subtypes from peripheral blood samples, PBMCs were first separated into a CD4+ memory cell fraction and remaining cells by use of the Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec). Both populations were stained with cocktails of fluorescently conjugated antibodies (see Supplementary Table 2) and sorted on a FACSAria-II (Becton Dickinson) using the gating strategy shown in Supplementary Fig. 2. Sorted cells were washed, and directly lysed in TRIzol solution (Invitrogen) for subsequent isolation of total RNA or fixed for ChIP assays as described previously14.
Gene expression studies
Total RNA was extracted using the miRNeasy Micro Kit (Qiagen); complementary DNA was reverse-transcribed with the SuperScript III First-Strand Synthesis System (Life Technologies). Real-time PCR employed the Fast Start Universal SYBR Green Master Mix (Roche); see Supplementary Table 1 for primer sequences. Data were acquired on the StepOnePlus Real-Time PCR System (Applied Biosystems); all results are presented in arbitrary units relative to expression of the housekeeping gene YWHAZ59.
Micro-scaled ChIP-Seq for H3K27ac and bioinformatics analysis
Samples from an independent cohort of 38 donors (as described above) were utilized for this analysis (Supplementary Data set 3). A total of 43 ChIP-Seq assays (including 5 technical replicates) were performed as described previously14,60. Briefly, purified chromatin from 1 × 105 TH2 cell enriched CD4+ memory subset (CD3+CD4+ CD25−CD45RA−CCR4+) was immunoprecipitated with a polyclonal anti-H3K27ac antibody (Lot #GR184333-1; ab4729; Abcam). Chromatin was incubated with 0.5 μg antibody pre-coated to 5 μl of protein A-coated magnetic beads (Invitrogen). Immunocomplexes were captured, washed and eluted; DNA was purified and used for whole-genome amplification as described previously14. The samples then underwent library preparation using the Illumina TruSeq Nano DNA Library Prep Kit following the manufacturer's instructions. Libraries were sequenced on an Illumina HiSeq 2500 sequencer to obtain >10 million uniquely mapped 50-bp single-end reads.
ChIP-Seq data was processed as described14. To generate the H3K27ac tracks shown in Fig. 3b, sequencing coverage was calculated for the six regions (+/−1 kb around the SNP site of interest) at 50 bp windows after extending each read to a length of 250 bp along the sequencing direction using MEDIPS v.1.10.0 (extend=250, uniq=F, window_size=50, BSgenome=‘BSgenome.Hsapiens.UCSC.hg19’), and the resulting coverage profiles (normalized reads counts, RPKM) were presented. Further, to calculate fold change in H3K27ac enrichment levels between carriers of risk and non-risk allele at genomic regions harbouring the SNPs of interest (shown in Fig. 3c), we used the MEDIPS bioinformatics pipeline (as above) to obtain normalized reads counts for a 200 bp region around the SNP site (see Supplementary Data set 4). Genome-wide analysis of this entire data set will be reported in a separate manuscript (under preparation).
CTCF-ChIP and analysis of allele-specific binding
Purified chromatin from 10 × 106 polarized TH1 and TH2 cells (31 samples from 11 donors) and 1 × 106 primary CD8+ T cells (13 samples from 9 donors) were immunoprecipitated with a polyclonal anti-CTCF antibody (Lot #2142232; 07-729; EMD Millipore), resulting in a total of 44 assays (including 13 technical duplicates). Chromatin was incubated with 10 μl or 5 μl antibody solution (for 10 × 106 cells or 1 × 106 cells ChIP, respectively), pre-coated with protein A-coated magnetic beads (Invitrogen). Immunocomplexes were captured, washed, and eluted and DNA was purified as described above. DNA sequences from control and target sites were quantified by real-time PCR (see Supplementary Table 1) to assess enrichment due to CTCF binding. Data are presented as fold enrichment over control region, which has previously been shown to not bind CTCF61. The donors were categorized (into homozygous risk, heterozygous and homozygous non-risk) based on allelic status of SNP overlapping the respective CTCF motif as shown in Fig. 4a and Supplementary Data set 3.
For allele-specific analysis, purified chromatin from input and CTCF-ChIP samples were amplified by PCR using primers that cover the CTCF-binding site overlapping SNPs rs12936231 (G/C; ZPBP2 site) and rs4065275 (A/G; ORMDL3 site), and analysed using the Sanger sequencing. Using MacVector, chromatogram traces of donors heterozygous for asthma risk SNPs (as above) were analysed to determine allele-preference for CTCF. The ratio of each allele was calculated using the signal intensity of each base at the position of the respective SNP. Data are shown as percentage of DNA sequences containing the risk and non-risk SNPs at rs12936231 (ZPBP2 site) or rs4065275 (ORMDL3 site).
Analyses of DHS and overlap with 17q21 SNPs
To define the collection of 17q21 asthma-risk SNPs, we first downloaded all asthma-associated SNPs from the databases of GWAS Integrator62 and HaploReg v3 (ref. 63) (August 2015), yielding a total of 96 significant lead SNPs (P<1.0 × 10−5) distributed over 75 loci (with 6 lead SNPs in the 17q21 locus). SNPs in tight genetic linkage (r2>0.8) were retrieved based on data from the Phase I of the 1,000 genome project using European (EUR) as reference population64 (calculations performed using HaploReg v3, Supplementary Data set 1). Among the total of 3,502 asthma-associated SNPs (lead SNPs+linked SNPs), 136 SNPs were identified in the asthma-susceptibility locus on chromosome 17 (17q21).
To determine the number of DHS in the asthma loci, we downloaded genomic annotations of DHS from 64 primary cells types, 21 fetal tissues and 28 cell lines provided by the ENCODE Project Consortium (ENCODE Encyclopedia, version 2; http://www.encodeproject.org)31 and the NIH Epigenomics Roadmap Consortium (http://www.roadmapepigenomics.org)32, and generated by the labs of Stamatoyannopoulos (University of Washington) and Crawford (Duke University). We utilized processed data provided by the ENOCDE project; here the Stam lab merged all DNase peak data from the Stam and Crawford labs. This merging process formed one combined DNase-Seq data set with non-overlapping DHS. The Stam lab then identified the ‘master’ peak in each region, defined as the peak in the region with highest peak height/z-score. Utilizing this data set, loci were defined by the location of the respective SNPs extended for 10 kb at each end. We found a total of 462 unique DHS in the 17q21 asthma-risk locus (chr17: 37,899,254–38,139,253 (hg19); 240 kb).
Next, to determine the average number of DHS in all loci for each cell type, we merged replicates from cell types of similar origin (see details in Supplementary Data set 2a). Of note, merged tracks of mixed or parental populations (for example data sets on ‘CD3 cells’ or ‘CD4 primary cells’) were excluded when data on subsets (such as ‘CD4 naive cells’, ‘TH1 cells’ and ‘CD8 cells’) were available. Our merged data set was used to determine the number of DHS within each cell type or tissue group (shown in Fig. 1d and Supplementary Fig. 1b) and to identify DHS that intersect directly with 17q21 SNPs (shown in Fig. 1e,f). The full list of merged DHS, including the number of single tracks for each cell type or tissue group, and the distribution of DHS in all asthma-associated loci (each defined by the location of the respective SNPs extended for 10 kb at each end) are provided in Supplementary Data set 2.
To predict the functional SNPs in the 17q21 locus, we performed motif scan analysis for the genomics regions highlighted in Fig. 3a using FIMO at the default parameters (P<1 × 10−4), implementing the motif models from the JASPAR database65 (see details Supplementary Data set 5).
4C-Seq assay and analysis
The 4C-Seq assay was used to determine regions interacting with the ORMDL3 promoter region40,41. Briefly, naive CD4+ T cells (or HUT-78 cells) were cross-linked and flash frozen. Cells were lysed, chromatin was digested using the 4-base cutter DpnII and restricted ends re-ligated in situ as described36. The chromatin was de-crosslinked and digested using the 4-base cutter MseI (or NlaIII) in order to produce small products40. Following a second ligation step in a large reaction volume (to minimize inter-molecular ligations), DNA sequences containing the ORMDL3 promoter region (anchor point/bait) were amplified by PCR (see Supplementary Table 1) and sequenced to generate 1–2 million 150 bp single-end reads per sample. Conditions were initially optimized in HUT-78 cells using five different baits located in the ORMDL3 promoter region (baits A-E), and bait D, which showed most interactions, was chosen to perform the 4C-Seq assay in primary CD4+ T cells.
Raw fastq files were filtered to remove reads that do not contain the expected bait sequence. From the remaining reads, the bait sequence was removed and reads were mapped against the genomic sequence (hg19) using either Bowtie 1.1.2 (4C-ker42) or the custom 4Cseqpipe40 mapper (perl 4cseqpipe.pl -map). Number of reads per each restriction enzyme fragment was computed using coverageBed (bedtools) on the fragmented genome (Supplementary Data set 7). Regions with significant interactions were calculated using 4C-ker (near-cis function with K=5). Differentially interacting regions at an adjusted P value of 0.1 were called using the differential analysis function of the 4C-ker tool (Supplementary Data set 6). All domainograms (for example, Fig. 5c) were generated using the 4Cseqpipe tool (near-cis parameter; trend resolution of 5,000; using mean as the statistics).
Cell culture and stimulation
For siRNA knockdown studies memory CD4+ T cells were cultured in Iscove's Modified Dulbecco's Medium (Invitrogen) supplemented with 5% (vol/vol) heat-inactivated fetal bovine serum and 2% (vol/vol) human AB serum (CellGro) with recombinant human IL-7 (5 ng ml−1; Miltenyi Biotec). For in vitro differentiation of naive cells into TH1 or TH2 cells, FACS-sorted naive CD4+ T cells were cultured with Human T-Activator CD3/CD28 Dynabeads (Invitrogen) at a bead-to-cell ratio of 1:1 in the presence of recombinant human IL-12 (rhIL-12; 5 ng ml−1) and anti-IL-4 antibody (5 μg ml−1), or recombinant human IL-4 (rhIL-4; 10 ng ml−1) and anti-IFN-γ antibody (10 μg ml−1), respectively. Recombinant IL-4, IL-12 and anti-IL-4 antibody were from R&D Systems; the anti-IFN-γ antibody was obtained from BD Pharmingen. After 48 h of culture, the anti-CD3/CD28 Dynabeads were removed and the cells were expanded in culture with recombinant human IL-2 (rhIL-2; 100 IU ml−1; National Cancer Institute). After 6 days, cells were washed and analysed for gene expression by real-time PCR. Memory CD4+ T cells were intracellularly stained for cytokine detection after stimulation with anti-CD3/CD28 Dynabeads for 6 and 18 h. Brefeldin A (5 μg ml−1; Sigma-Aldrich) was added for the final 2 h of culture. The intracellular staining assay was performed as previously described60. Data were acquired on a LSR-II (Becton Dickinson) and analysed using FlowJo software (Tree Star).
siRNA knockdown studies
Memory CD4+ T cells were transiently transfected with 0.5 nmol (per 1 × 106 cells) of siRNA pools specific for ORMDL3, GSDMB, TBX21, and GATA3 or non-targeting siRNA (all ON-TARGETplus SMARTpools from Dharmacon) using the Neon Transfection System (Invitrogen) according to the manufacturer's protocol (settings: 2,200 V, 10 ms, 3 pulses). All siRNA sequences are provided in Supplementary Table 3. Knockdown efficiency was analysed 48 h after transfection by real-time PCR for transcript levels, and by intracellular FACS assay for protein levels when specific and good antibodies were available for ORMDL (ab107639) as previously described45, T-bet (4B10) and GATA3 (L50-823) (Fig. 6b and Supplementary Fig. 6a,b); we also verified the effects of ORMDL3 and GSDMB siRNA pools by using the four independent siRNAs in the SMARTpool and the corresponding C911 mismatch control siRNAs66, where the nucleotide sequence is altered at bases 9–11, the seed sequence needed for specific targeting (Supplementary Fig. 6e,f and Supplementary Table 3). Viability of cells was analysed 48 h after transfection by using FACS-based Annexin-V and 4′,6-diamidino-2-phenylindole (DAPI) staining. Subsequently, the memory CD4+ T cells were stimulated with anti-CD3/CD28 Dynabeads and cytokine production was analysed by real-time PCR and intracellular FACS at various time points. Cytokine secretion after 48 h of stimulation was measured with V-PLEX Validated Assay Kits (Meso Scale Discovery) following the manufacturer’s instructions. All data are presented as mean of biological duplicates.
Data availability
The authors declare that the data supporting the findings of this study are available within the paper (and its supplementary information files).
Additional information
How to cite this article: Schmiedel, B. J. et al. 17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells. Nat. Commun. 7, 13426 doi: 10.1038/ncomms13426 (2016).
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Acknowledgements
We thank Cheryl Kim, Lara Nosworthy and Kurt van Gunst at the La Jolla Institute (LJI) flow cytometry facility for assisting with cell sorting; Alex Fu, Moghaddas Gholami and Jason Greenbaum at the LJI bioinformatics core for help with processing and analysis of sequencing data; the ENCODE Project and NIH Epigenomics Roadmap Consortiums, the laboratories of Stamatoyannopoulos (University of Washington), Crawford (Duke University) and Bernstein and Meissner (Broad Institute) for generating publically available data sets on DHS, H3K27ac and H3K4me1 enrichment; and Nunzio Bottini, Anjana Rao and Jessica Moore for careful reviewing of the manuscript. This work was funded by NIH grants R01 HL114093 and R24 AI108564 (P.V., B.P.), U19 AI100275 (P.V., B.P., A.S.) and The William K. Bowes Jr Foundation (P.V.).
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B.J.S., A.S., B.P. and P.V. conceived the work, designed, performed and analysed experiments, and wrote the paper; D.S.-C. performed bioinformatic analyses under the supervision of B.J.S., L.C. and P.V.; F.A. supervised the analysis of 4C-Seq data and provided statistical input for the bioinformatics analysis; J.C. performed micro-scaled ChIP-Seq experiments under the supervision of G.S. and P.V.; V.S. performed T cell assays.
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Supplementary information
Supplementary Information
Supplementary Figures 1-6, Supplementary Tables 1-3 and Supplementary References (PDF 1436 kb)
Supplementary Data 1
GWAS-derived asthma-associated lead SNPs (highlighted in gray) and SNPs in tight genetic linkage. (XLSX 156 kb)
Supplementary Data 2
Supplementary Dataset 2A: Analysis of publically available DNase-Seq datasets (ENCODE Project Consortium; see Methods), DNase hypersensitivity sites (DHS) in 17q21 locus in primary cell types (n=212), fetal tissues (n=279) and cell lines (n=107); in alphabetical order. Name of cell type/tissue, sample title (as obtained from ENCODE master file) of unique tracks used for the grouping, total number of DHS genome-wide and in the 17q21 locus of each track. Supplementary Dataset 2B: DNase hypersensitivity sites (DHS) in 17q21 locus in primary cell types (immune cell types used in analysis (n=10) printed in bold), fetal tissues and cell lines; ordered by number of DHS in 17q21 locus. Number of unique tracks used for merging cells and tissue of similar origin, average number of DHS genome-wide and in the 17q21 locus, and number of DHS overlapping with 17q21 SNPs (Fig. 1). Supplementary Dataset 2C: Average number of DNase hypersensitivity sites (DHS) in asthma-associated loci (n=75) in primary cell types (immune cell types used for analysis (n=10) printed in bold), fetal tissues and cell lines (see Methods). Loci are named by a prominent lead SNP and ordered by the calculated ratio of immune versus non-immune cell types; cell types in alphabetical order. (XLSX 110 kb)
Supplementary Data 3
Age and gender of study subjects, along with their classification based on the genotype for rs7216389. Genotype status of other linked SNPs (rs12936231, rs4065275) that overlap CTCF binding sites is also shown. (XLSX 17 kb)
Supplementary Data 4
Normalized counts for H3K27ac enrichment (RPKM) in 400 bp window around the 17q21 SNPs of interest (200 bp region on either side; see Methods) for homozygous risk (rs7216389 T/T, n=36) and non-risk (rs7216389 C/C, n=7) samples. The P value was calculated using the Mann-Whitney U test. (XLSX 22 kb)
Supplementary Data 5
Prediction of TF binding sites affected by 17q21 SNPs. List of 17q21 asthma-risk SNPs overlapping DHS (described in Fig. 3a) that affect transcription factor (TF) binding motifs. The list shows the respective 17q21 SNPs (for asthma-risk and non-risk allele), the name of the TF along with its motif (the nucleotide of the 17q21 SNP is illustrated in bold font), the motif scores, P values from FIMO analysis. Missing entries indicate that the predicted TF binding site was only found on one allele by the FIMO analysis (using default settings). (XLSX 17 kb)
Supplementary Data 6
Normalized counts and P values from the 4C-Seq analysis of the ORMDL3 promoter region (chr17: 37,849,238 - 38,189,238 (hg19); 340 kb) using the 4C-ker method, ordered by adjusted P values. Significantly differing regions are highlighted in red (up in risk) and blue (up on non-risk). (XLSX 54 kb)
Supplementary Data 7
Raw read counts from 4C-Seq analysis of the ORMDL3 promoter region (chr17: 37,849,238 - 38,189,238 (hg19); 340 kb), ordered by chromosomal location. Data were filtered to include only regions with a sum of >50 reads across all donors. Regions enriched in at least 3 of 4 donors for risk or non-risk alleles are highlighted (red or blue, respectively). (XLSX 79 kb)
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Schmiedel, B., Seumois, G., Samaniego-Castruita, D. et al. 17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells. Nat Commun 7, 13426 (2016). https://doi.org/10.1038/ncomms13426
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DOI: https://doi.org/10.1038/ncomms13426
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