Abstract
We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8*) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides GM1 and GD1a. Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses.
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Acknowledgements
We thank K. Hidari (University of Shizuoka) for the gift of GD1a, A. Bugarcic (Griffith University) and M. Kraschnefski (Griffith University) for preparation of Wa VP8* protein, and A. Szyczew (Griffith University) for production of Neu5Acα2Me. B.S.C., M.J.K., H.B. and M.v.I. gratefully acknowledge the financial support of the Australian Research Council (ARC). B.S.C. thanks the National Health and Medical Research Council of Australia (NHMRC) for the award of a Senior Research Fellowship. M.v.I. thanks the ARC for the award of an Australian Federation Fellowship and the NHMRC for financial support.
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Haselhorst, T., Fleming, F., Dyason, J. et al. Sialic acid dependence in rotavirus host cell invasion. Nat Chem Biol 5, 91–93 (2009). https://doi.org/10.1038/nchembio.134
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DOI: https://doi.org/10.1038/nchembio.134
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