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Chromatographic Removal of Pyrogens

Bio/Technology volume 2, pages 1035–1038 (1984)Cite this article

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References

  1. Recombinant DNA Committee, “Points to Consider in the Production and Testing of New Drugs and Biologicals Produced by Recombinant DNA Technology”. Nov. 18, 1983.

  2. Dinarello, C.A., Bishai, I., Rosen-wasser, L.J., Coceani, F. 1984. The influence of lip oxygenase inhibitors on the in vitro production of human leukocyte pyrogen and lymphocyte activating factor interleukin 1. Int. J. Immunopharmacol. 6: 43–50.

    Article  CAS  Google Scholar 

  3. Curling, J.M., Cooney, J.M. 1982. Operation of large scale gel filtration and ion-exchange systems. J. Parenteral Science and Technology 36: 59–63.

    CAS  Google Scholar 

  4. Friedli, H., Kistler, P. 1980. Removal of ethanol and salt from albumin by gel filtration, p. 203–210. In: Methods of plasma protein fractionation, J. M. Curling, (ed.), Academic Press, New York.

    Google Scholar 

  5. Sarafin, T.A., Tsay, K.K., Fluharty, A.L., Kihara, H. 1982. A procedure for pyrogen decontamination of Sephacryl S-300. Biochemical Medicine 28: 237–240.

    Article  Google Scholar 

  6. Pharmacia Fine Chemicals 1984. Pharmacia Bioprocess Columns Instruction Manual.

  7. Personal communication, Tere Kristiansen.

  8. Block, S.S. (ed.) 1977. Sterilization and preservation. Lea and Febiger, Philadelphia.

    Google Scholar 

  9. Grabner, R.W. 1975. Process for removing pyrogenic material from aqueous solutions. U. S. Patent 3,897,309.

    Google Scholar 

  10. Shibatani, T., Kakimoto, T., Chibata, I. 1983. Purification of high molecular weight urokinase from human urine and comparative study of two active forms of urokinase. Thromb. Haemostasis. 49: 91–95.

    CAS  Google Scholar 

  11. Dembinski, W., O'Malley, J.A., Sulkowski, E. 1983. Large scale purification procedure for human fibroblast interferon. Interferon Scientific Memoranda. Jan/Feb. ; p. 6.

  12. Bishop, D.F., Desnick, R.J. 1981. Affinity purification of alpha galactosidase A EC-3.2.1.22 from human spleen, placenta and plasma with elimination of pyrogen contamination properties of the purified splenic enzyme compared to other forms. J. Biol. Chem. 256: 1307–1316.

    CAS  PubMed  Google Scholar 

  13. Borg, H., Lundgren, E., Hollenberg, M.D., Veale, W.L., Tan, Y.H., McPherson, A. 1981. Human interferon for clinical trials removal of pyrogen by a simple 2-step procedure. Can. J. Physiol. Pharmacol. 59: 890–892.

    Article  CAS  Google Scholar 

  14. Duff, G.W., Waisman, D.M., Atkins, E. 1982. Removal of endotoxin by a polymixin B affinity column. Clin. Res. 30: 565A.

    Google Scholar 

  15. Morrison, D.C., Jacobs, D.M. 1976. Binding of polymixin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry 13: 813–818.

    Article  CAS  Google Scholar 

  16. Issekutz, A.C. 1983. Removal of gram-negative endotoxin from solutions by affinity chromatography. J. Immunol. Methods 61: 275–281.

    Article  CAS  Google Scholar 

  17. Chibata, I. 1983. Method for reducing pyrogen content of or removing pyrogens from substances contaminated therewith. U. S. Patent 4,381,239.

    Google Scholar 

  18. Minobe, S., Tadashi, S., Tosa, T., Chibata, I. 1983. Characteristics of immobilized histamine for pyrogen adsorption. J. Chromatogr. 262: 193–198.

    Article  CAS  Google Scholar 

  19. Minobe, S., Watanabe, T., Sato, T., Tosa, T., Ichiro, C. 1983. Preparation of adsorbents for pyrogen adsorption. J. Chromatogr. 248: 401–408.

    Article  Google Scholar 

  20. Shadduck, R.K., Waheed, A., Porcellini, A., Rizzoli, V., Levin, J. 1980. A method for the removal of endotoxin from purified colony-stimulating factor. Proc. Soc. Exp. Biol. Med. 164: 40–50.

    Article  CAS  Google Scholar 

  21. Yanchinski, S. 1984. Fermentech developing Protein A process. Bio/Technology 2: 667–668.

    Google Scholar 

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Authors and Affiliations

  1. the senior product specialist-chromatography at Pharmacia Fine Chemicals, 800 Centennial Ave., Piscataway, NJ, 08854

    Gail Sofer

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  1. Gail Sofer
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Sofer, G. Chromatographic Removal of Pyrogens. Nat Biotechnol 2, 1035–1038 (1984). https://doi.org/10.1038/nbt1284-1035

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