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The simplicity of complex MACs

Nature Biotechnology volume 15, pages 1257–1259 (1997)Cite this article

Abstract

The development of mammalian artificial chromosomes (MACs) would be useful for biotechnology and biomediclne, including their use in functional genomics, transgenic animals and gene therapy. By analogy to large cloning systems in microorganisms, MACs may be engineered using endogenous chromosomal elements such as the yeast-based artificial chromosomes (YACs), or exogenous extra-chromosomal components derived from viruses and other cellular parasites such as the bacterial-based artificial chromosomes (BACs) and p1 artificial chromosomes (PACs).

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References

  1. Fair, C.J., Bayne, R.A., Kipling, D., Mills, W., Critcher, F., and Cooke, H.J. 1995. Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation. EMBO 14: 5444–5454.

    Article  Google Scholar 

  2. Heller, R., Brown, K.E., Burgtorf, C., and Brown, W.R. 1996. Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage. Proc. Natl. Acad. Sci. USA. 93: 7125–7130.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Sun, T.-Q., Fenstermacher, D., and Vos, J.-M.H. 1994.Human artificial episomal chromosomes for cloning large DNA in human cells. Nature Genet. 8: 33–41.

    Article  CAS  PubMed  Google Scholar 

  4. Simpson, K., McGuigan, A., and Huxley, C. 1996. Stable episomal maintenance of yeast artificial chromosomes in human cells. Mol. Cell. Biol. 16: 5117–5126.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Banerjee, S., Livanos, L., and Vos, J.-M.H. 1995. Therapeutic gene delivery in human B-lymphoblastoid cells by engineered non-transforming Epstein-Barr virus. Nature Medicine 1: 1303–1308.

    Article  CAS  PubMed  Google Scholar 

  6. Wang, F., Xiangping, L., Annis, B., and Faustman, D.L. 1995.Tap-1 and Tap-2 gene therapy selectively restores conformationally dependent HLA class I expression in type I diabetic cells. Hum. Gene Then 6: 1005–1017.

    Article  CAS  Google Scholar 

  7. Sun, T.-Q., Livanos, E., and Vos, J.-M.H. 1996.Engineering a mini-herpesvirus as a general strategy to transduce up to 180 kb of functional self-replicating human mini-chromosomes. Gene Ther. 3: 1081–1088.

    CAS  PubMed  Google Scholar 

  8. Featherstone, T. and Huxley, C. 1993. Extrachromosomal maintenance and amplification of yeast artificial chromosome DNA in mouse cells. Genomics 17: 267–278.

    Article  CAS  PubMed  Google Scholar 

  9. Nonet, G.H. and Wahl, G.M. 1993. Introduction of YACs containing a putative mammalian replication origin into mammalian cells can generate structures that replicate autonomously. Somatic Cell and Mol. Genet. 19: 171–192.

    Article  CAS  Google Scholar 

  10. Harrington, J.J., Bokkelen, G.V., Mays, R.W., Gustashaw, K., and Willard, H.F. 1997. Formation for de novo centromeres and construction of first-generation human artificial microchromosomes. Nature Gen. 15: 345–355.

    Article  CAS  Google Scholar 

  11. Kipling, D. and Warburton, P.E. 1997. Centromeres, CENP-B and Tigger too. Trends in Genet. 13: 141–145.

    Article  CAS  Google Scholar 

  12. Brown, W. and Tyler-Smith, C. 1995. Centromere activation. Trends in Genet. 11: 337–339.

    Article  CAS  Google Scholar 

  13. du Sart, D., Cancilla, M.R., Earle, E., Mao, J.-l., Safferey, R., Tainton, K.M., et al. 1997. A functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA. Nature Gen. In press.

    Google Scholar 

  14. Kosztolanyi, G., Mehes, K., and Hook, E.B. 1991. Inherited ring chromosomes: an analysis of published cases. Hum. Gen. 87: 320–324.

    Article  CAS  Google Scholar 

  15. DePamphilis, M.L. Origins of DNA replication, pp. 45–87 in DNA replication in eukaryotic cells. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1996).

  16. Tomizuka, Yoshida, H., Uejima, H., Hiroyuki, K., Sato, K., Ohguma, A., et al. 1997. Functional expression and germline transmission of a human chromosome fragment in chimaeric mice. Nature Genet. 16: 133–143.

    Article  PubMed  Google Scholar 

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  1. Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-7295

    Jean-Michel H. Vos

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  1. Jean-Michel H. Vos
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Vos, JM. The simplicity of complex MACs. Nat Biotechnol 15, 1257–1259 (1997). https://doi.org/10.1038/nbt1197-1257

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