Abstract
We report a simple, direct gene transfer technique based on sonication. Transient expression of a β–glucoronidase (GUS) gene was detected in 86 percent of treated leaf segments of Nicotiana tabacum, and the area of transformed tissue on these segments reached 60–70 percent. GUS activity in the leaves of regenerated, kanamycin resistant plantlets was found at a frequency of 22 percent, and analysis of R1 regenerated seedlings showed that both kanamycin resistance and GUS behaved as typical linked dominant genes.
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References
Xu, N., Zhao, N.-M., Zhang, L.-J., Chen, L.-M. and Jia, S.-R. 1990. Gene transfer by sonication. Acta Biophysica Sinica 6(2): 281.
Joersbo, M. and Brunstedt, J. 1990. Direct gene transfer to plant protoplasts by mild sonication. Plant Cell Rep. 9: 207–210.
Töpfer, R., Gronenborn, B., Schell, J. and Steinbiss, H-H 1989. Uptake and transient expression of chimeric genes in seed derived embryos. The Plant Cell 1: 133–139.
Murashige, T. and Skong, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 437–497.
Jefferson, R.A. 1987. Assaying chimeric genes in plants: the β-glucuronidase as a versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907.
Jia, S.-R., Yang, M.-Z., Ott, R. and Chua,N.-H. 1989. High frequency transformation of Kalanchoe laciniata. Plant Cell Rep. 8: 336–340.
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Zhang, LJ., Cheng, LM., Xu, N. et al. Efficient Transformation of Tobacco by Ultrasonication. Nat Biotechnol 9, 996–997 (1991). https://doi.org/10.1038/nbt1091-996
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DOI: https://doi.org/10.1038/nbt1091-996
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