Abstract
A pathogenic strain of Vibrio cholerae was attenuated by deletion of DNA sequences encoding the A1 subunit of the cholera enterotoxin. A restriction endonuclease fragment encoding the A1, but not the A2 or B sequences was deleted in vitro from cloned cholera toxin genes. The mutation was then recombined into the chromosome of V.cholerae Ogawa 395, a pathogenic strain that confers complete immunity to subsequent infection following an initial clinical infection. The resulting strain, which produces the immunogenic but nontoxic B subunit of cholera toxin and is unaltered in other critical antigens, represents a promising candidate for an attenuated live oral cholera vaccine.
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Kaper, J., Lockman, H., Baldini, M. et al. A Recombinant Live Oral Cholera Vaccine. Nat Biotechnol 2, 345–349 (1984). https://doi.org/10.1038/nbt0484-345
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DOI: https://doi.org/10.1038/nbt0484-345
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