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Production of Human Interleukin-3 Using Industrial Microorganisms

Bio/Technology volume 9, pages 47–52 (1991)Cite this article

Abstract

We expressed a cDNA encoding the multi-colony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, we designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.

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Author notes

  1. Rob W. van Leen: Corresponding author.

Authors and Affiliations

  1. Royal Gist-brocades NV, P.O. Box 1, 2600 MA, Delft, The Netherlands

    Rob W. van Leen, Janny G. Bakhuis, Ruud F. W. C. van Beckhoven, Ronald W. J. Hommes, Patty J. Lemson, Bert Noordam & Niek L. M. Persoon

  2. Section Experimental Hematology, ITRI-TNO, Rijswijk, The Netherlands

    Herman Burger

  3. Dr. Daniel den Hoed Kliniek, P.O. Box 5201, 3008 EA, Rotterdam, The Netherlands

    Lambert C. J. Dorssers

  4. Institute of Radiobiology, Erasmus University, Rotterdam and Section Experimental Hematology, ITRI-TNO, Rijswijk, The Netherlands

    Gerard Wagemaker

Authors
  1. Rob W. van Leen
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  2. Janny G. Bakhuis
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  3. Ruud F. W. C. van Beckhoven
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  4. Herman Burger
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  5. Lambert C. J. Dorssers
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  6. Ronald W. J. Hommes
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  7. Patty J. Lemson
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  8. Bert Noordam
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  9. Niek L. M. Persoon
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  10. Gerard Wagemaker
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van Leen, R., Bakhuis, J., van Beckhoven, R. et al. Production of Human Interleukin-3 Using Industrial Microorganisms. Nat Biotechnol 9, 47–52 (1991). https://doi.org/10.1038/nbt0191-47

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  • DOI: https://doi.org/10.1038/nbt0191-47

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