Papers from the literature selected by the Nature Biotechnology editors (follow us on Twitter, @NatureBiotech #nbtHighlight)

Maintenance of hematopoietic stem cells through regulation of Wnt and mTOR pathways

Huang, J. et al. Nat. Med. doi:10.1038/nm.2984 (11 November 2012)

A physical, genetic and functional sequence assembly of the barley genome

The International Barley Genome Sequencing Consortium. Nature doi:10.1038/nature11543 (17 October 2012)

Efficient TALEN-mediated gene knockout in livestock

Carlson, D.F. et al. PNAS 109, 17382–17387 (2012)

Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy

Chojnacki, J. et al. Science 338, 524–528 (2012)

DNA template strand sequencing of single-cells maps genomic rearrangements at high resolution

Falconer, E. et al. Nat. Methods 9, 1107–1112 (2012)