Abstract
The technique of partial hepatectomy is widely used to model liver regeneration and cell cycle dynamics in vivo. Because murine gene expression can be manipulated relatively easily, partial hepatectomy in mice is a useful tool for exploring the contributions of different genes to such hepatic processes. The authors present a straightforward method of partial hepatectomy in the mouse.
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References
Higgins, G. & Anderson, R.M. Experimental pathology of the liver. I. Restoration of the liver of the white rat following surgical removal. Arch. Pathol. 12, 186–206 (1931).
Greene, A.K. & Puder, M. Partial hepatectomy in the mouse: technique and perioperative management. J. Invest. Surg. 16, 99–102 (2003).
Martins, P.N., Theruvath, T.P. & Neuhaus, P. Rodent models of partial hepatectomies. Liver Int. 28, 3–11 (2008).
Martins, P.N. & Neuhaus, P. Hepatic lobectomy and segmentectomy models using microsurgical techniques. Microsurgery 28, 187–191 (2008).
Koniaris, L.G., McKillop, I.H., Schwartz, S.I. & Zimmers, T.A. Liver regeneration. J. Am. Coll. Surg. 197, 634–659 (2003).
Martins, P.N. & Neuhaus, P. Surgical anatomy of the liver, hepatic vasculature and bile ducts in the rat. Liver Int. 27, 384–392 (2007).
Bucher, N.L. & Swaffield, M.N. The rate of incorporation of labeled thymidine into the deoxyribonucleic acid of regenerating rat liver in relation to the amount of liver excised. Cancer Res. 24, 1611–1625 (1964).
Desbarats, J. & Newell, M.K. Fas engagement accelerates liver regeneration after partial hepatectomy. Nat. Med. 6, 920–923 (2000).
Albrecht, J.H., Meyer, A.H. & Hu, M.Y. Regulation of cyclin-dependent kinase inhibitor p21(WAF1/Cip1/Sdi1) gene expression in hepatic regeneration. Hepatology 25, 557–563 (1997).
Jaime, M. et al. The p21(Cip1) protein, a cyclin inhibitor, regulates the levels and the intracellular localization of CDC25A in mice regenerating livers. Hepatology 35, 1063–1071 (2002).
Waynforth, H.B. & Flecknell, P.A. Experimental and Surgical Technique in the Rat 241 (Academic, San Diego, 1992).
Acknowledgements
This work was supported by a Fellowship from Medical Research Scotland.
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Boyce, S., Harrison, D. A detailed methodology of partial hepatectomy in the mouse. Lab Anim 37, 529–532 (2008). https://doi.org/10.1038/laban1108-529
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DOI: https://doi.org/10.1038/laban1108-529
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